Separation of the Transforming and Viral Deoxyribonucleic Acids of a Transducing Bacteriophage of Bacillus Subtilis

Okubo, Satoshi; Stodolsky, Marvin; Bott, Kenneth F.; Strauss, Bernard S.

doi:10.1073/pnas.50.4.679

Cited by 36 publications

(33 citation statements)

References 16 publications

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“…Ikeda & Tomizawa (1965) studied transduction by phage P 1 and concluded that' most of the transducing particles lack phage genome and carry only fragments 10 K. W. HEDGES of the bacterial chromosome'. Similarly, Okubo et al (1963) and Mahler, Cahoon & Marmur (1964) found that the bacterial DNA isolated from lysates of transducing phages of Bacillus subtilis was not covalently bonded to phage DNA. Yet other workers have clearly demonstrated that phage P 1 can produce transducing particles in which a segment of DNA from the bacterial chromosome becomes integrated into the genome of the transducing phage.…”

Section: Introductionmentioning

confidence: 93%

Transduction mechanisms of bacteriophage ε¹⁵I. General properties of the system

Hedges¹

1971

Genet. Res.

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Bacteriophage e? is capable of transduction both by replacement of a genetic segment of the recipient by the homologous genetic material from the donor strain and by the formation of defective transducing particles capable of lysogenizing the recipient strain of 8. anatum.The isolation of strains carrying such prophages, which have incorporated the lactose or arabinose operons, is reported. Lysogenic strains, carrying both normal and defective transducing prophage, form highfrequency transducing lysates. Other strains, carrying only defective prophage, show evidence that the association of prophage genes and transduced materials is stable since the loss of one frequently entails loss of the other.

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Section: Introductionmentioning

confidence: 93%

Transduction mechanisms of bacteriophage ε¹⁵I. General properties of the system

Hedges¹

1971

Genet. Res.

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“…Studies on PBS1 (355) show that the transducing particles in a lysate can be separated from infectious particles in CsCl, and the former contain only host DNA. Similarly, investigations of SP10 indicate transducing DNA is not covalently bound to phage DNA, and most likely resides in different virions (218). Probably some maturing PBS1 and SP10 phage particles inadvertently package pieces of host DNA.…”

Section: Transducing Phages and Pseudolysogenymentioning

confidence: 99%

Bacteriophages of Bacillus subtilis.

Hemphill¹,

Whiteley²

1975

Bacteriological Reviews

121

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“…It has been demonstrated that SPlO transducing particles contain no phage DNA (Okubo et al, 1963). This also seems to be the case with P 1 (Ikeda & Tomizawa, 1965) and PBS 1 (Yagamashi & Takahashi, 1968).…”

Section: Discussionmentioning

confidence: 72%

Molecular Origin of Transducing DNA in Bacteriophage SPP1

Lencastre¹,

Archer²

1981

Microbiology

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Transducing particles produced by bacteriophage SPP1 infection of Bacillus subtilis were separated from plaque-forming units by CsCl density-gradient centrifugation. The density in CsCl of DNA isolated from such purified transducing particles was similar to that of bacterial DNA, indicating that the transducing particles probably contain DNA of exclusively bacterial origin. Bacterial DNA synthesized after infection of the donor culture was also encapsulated in the transducing particles. The number of transducing particles was at least 10 times higher than that of the transductants.

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Separation of the Transforming and Viral Deoxyribonucleic Acids of a Transducing Bacteriophage of Bacillus Subtilis

Cited by 36 publications

References 16 publications

Transduction mechanisms of bacteriophage ε¹⁵I. General properties of the system

Transduction mechanisms of bacteriophage ε¹⁵I. General properties of the system

Bacteriophages of Bacillus subtilis.

Molecular Origin of Transducing DNA in Bacteriophage SPP1

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Separation of the Transforming and Viral Deoxyribonucleic Acids of a Transducing Bacteriophage of Bacillus Subtilis

Cited by 36 publications

References 16 publications

Transduction mechanisms of bacteriophage ε15I. General properties of the system

Transduction mechanisms of bacteriophage ε15I. General properties of the system

Bacteriophages of Bacillus subtilis.

Molecular Origin of Transducing DNA in Bacteriophage SPP1

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Transduction mechanisms of bacteriophage ε¹⁵I. General properties of the system

Transduction mechanisms of bacteriophage ε¹⁵I. General properties of the system