Separation of the citric acid cycle acids by liquid chromatography

Comparison of Gas-liquid and High-performance Liquid Chromatography, and Mass Spectrometry, for the Detection of Organic Acids in Culture Media of Azospirillum brasilense and Desulfovibrio desulfuricans

“…A further deterrent to the use of GLC/MS for routine analysis of organic acids is the cost of the instruments. Turkelson & Richards (1978) were able to separate all TCA cycle acids on a cation-exchange resin by eluting the column with dilute HC1. A modification of the above procedure was used to separate organic acids in dairy products by HPLC (Marsili et al, 1981).…”

Application of polymethacrylate resin as stationary phase in liquid chromatography with UV detection for C1–C7 aliphatic monocarboxylic acids and C1–C7 aliphatic monoamines

“…The separation of carboxylic acids mainly proceeds on a cation-exchange resin [low cross-linked styrenedivinylbenzene copolymer (PS-DVB)-based strongly acidic cation-exchange resin] in the H + form as the stationary phase with acidic solution as the eluent [5,6]. The separation of amines proceeds on anion-exchange resin (low cross-linked PS-DVB-based strongly basic anion-exchange resin) in the OH − form as the stationary phase with basic solution as the eluent [7,8].…”

“…6. Peaks: (1) methylamine; (2) ethylamine; (3) propylamine; (4) isobutylamine; (5) butylamine;(6) isoamylamine; (7) amylamine; (8) 1,3-dimethylbutylamine; (9) hexylamine; (10) 2-heptylamine and;(11) heptylamine.…”

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC) VI. Determination of lactic acid and short‐chain carboxylic acids C1–C5

“…Gas chromatography "7-141 has provided separations with high sensitivity, and has been used widely in the analysis of biological samples. Excellent gas-chromatographic separations between l+-and l--lactic acid have also been achieved [15].…”