Separation of synthetic food colourants by capillary zone electrophoresis in a hydrodynamically closed separation compartment

Masár, Marián; Kaniansky, Dušan; Madajová, V.

doi:10.1016/0021-9673(95)00938-8

Cited by 56 publications

(15 citation statements)

References 18 publications

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“…Although already reported by several authors (see, e. g., [22,[24][25][26][27][28][29][30]) this is not a widely employed alternative because enhanced thermal effects, associated with the use of such columns, result in reduced separation eficiencies 14, 61. Another possibility in improving the concentration limits of detection is the use of a more sensitive detection technique.…”

Section: Introductionmentioning

confidence: 97%

Capillary zone electrophoresis of inorganic anions with conductivity detection

1996

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A carrier electrolyte system for capillary zone electrophoresis (CZE) resolving chloride, bromide, iodide, fluoride, nitrite, nitrate, sulfate, and phosphate in a hydrodynamically closed separation compartment is described. The carrier electrolyte combines the effects of pH, polyvinylpyrrolidone (PVP) and the counterionic constituent on the effective mobilities of the anions. In 300 microns ID capillary tubes made of fluorinated ethylene-propylene copolymer (FEP), and with detection of analytes with the aid of an alternating current conductivity detector, detection limits in the range of 3-10 ppb could be achieved for 200 nL sample volumes. The separation efficiencies were in the range of 1.5-2.5 x 10(5) theoretical plates per meter for an adequate sample load. The reproducibility was evaluated for two concentration levels. For concentrations close to the limits of quantitation (50-120 ppb), the RSD values ranged from 1.5-12.6%, with the highest scatter for fluoride and phosphate. The RSD values were in the range of 0.4-1.5% for 300-1200 ppb concentrations of the anions. Typical analysis times were 2-5 min, depending on the anion species. A series of water samples (drinking, river, rain) was used to assess the practical applicability of the CZE method. The method is a suitable alternative for the determination of both anionic macro- and microconstituents in water with a good overall selectivity.

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Section: Introductionmentioning

confidence: 97%

Capillary zone electrophoresis of inorganic anions with conductivity detection

1996

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“…Tompson and Trenerry [16] analyzed some synthetic dyes (among them erythrosine B, tartrazine and cyanosine) in confectionery and Australian cordials using MEKC with sodium deoxycholate as surfactant additive in 20 min after sample extraction on a C18 Sep-Pak cartridge. Masar et al [23] quantified some synthetic food dyes, such as tartrazine, chromotrope FB, erythrosine B, etc., in the aperol liquor sample. They illustrated a new CE methodology using a 300 pm ID capillary which made it possible to reach the high detection sensitivity of 11-300 ppb.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of synthetic dyes in lipstick by micellar electrokinetic capillary chromatography

1998

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The separation of synthetic dyes, used as color additives in cosmetics, by micellar electrokinetic capillary chromatography (MEKC) is described in this study. The separation of seven dyes, namely eosine, erythrosine, cyanosine, rhodamine B, orange II, chromotrope FB and tartrazine has been achieved in about 3 min in an untreated fused silica capillary containing as background electrolyte a 25 mM tetraborate/phosphate buffer, pH 8.0, and 30 mM sodium dodecyl sulfate. The electrophoretic method exhibits precision and relatively high sensitivity. A detection limit (LOD, signal/noise = 3) in the range of 5-7.5 X 10(-7) M of standard compounds was recorded. Intra-day repeatability of all the studied dye determinations (8 runs) gave the following results (limit values), % standard deviation: 0.24-1.54% for migration time, 0.99-1.24% for corrected peak areas, 0.99-1.24% for corrected peak area ratio (analyte/internal standard) and 1.56-2.74% for peak areas. The optimized method was successfully applied to the analysis of a lipstick sample where eosine and cyanosine were present.

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“…Capillary electrophoresis (CE) is increasing regarded as an attractive separation technique because of its high efficiency, short analysis times and the requirement of small volumes of analytes and running solution additives, compared with those necessary for HPLC. Although several papers [7][8][9][10] have described the separation of dyes by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC), CE to the best of our knowledge has not been applied to the separation of fluorescein dyes. Because of the ionogenic nature of fluorescein and its derivatives at certain pHs, they are good candidates for separation by CZE.…”

Section: Introductionmentioning

confidence: 99%

Separation of fluorescein dyes by capillary electrophoresis using β-cyclodextrin

et al. 1998

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SummaryA capillary electrophoresis method for the separation and determination of five synthetic dyes used in pharmaceutical preparations, cosmetics and as food additives is described. The dyes, fluorescein, dichlorofluorescein, Rose Bengal, erythrosine and eosine are well separated in less than 12 min using an electrolyte of 50 mM phosphate buffer (pH 7.5), 10 mM [~-cyclodextrin and 5 % (v/v) methanol. A linear relationship between concentration and peak area for each dye was obtained in the concentration range 0.3-500 lag mL -1, with a correlation coefficient greater than 0.999. Intra-and inter-day precision of about 0.2-2.6 % RSD (n = 11) and 4.9-9.7 % RSD (n = 30), respectively, were obtained. The method has been used for determining the purity of fluorescein and erythrosine in practical samples.

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Separation of synthetic food colourants by capillary zone electrophoresis in a hydrodynamically closed separation compartment

Cited by 56 publications

References 18 publications

Capillary zone electrophoresis of inorganic anions with conductivity detection

Capillary zone electrophoresis of inorganic anions with conductivity detection

Quantitative analysis of synthetic dyes in lipstick by micellar electrokinetic capillary chromatography

Separation of fluorescein dyes by capillary electrophoresis using β-cyclodextrin

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