Separation of sugars by HPLC with electrochemical detection

Buchberger, Wolfgang; Winsauer, K.; Breitwieser, Ch.

doi:10.1007/bf00493800

Cited by 41 publications

(10 citation statements)

References 10 publications

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“…Nickel and nickel oxide electrodes present high catalytic activity in the oxidation of a wide range of organic compounds in alkaline solutions, though they are known to exhibit low selectivity . This shortcoming is circumvented when nickel electrodes are used in electrochemical detectors coupled to chromatography for the separation of complex samples .…”

Section: Introductionmentioning

confidence: 99%

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Determination of furanic aldehydes in sugarcane bagasse by high‐performance liquid chromatography with pulsed amperometric detection using a modified electrode with nickel nanoparticles

Silva

Beluomini

Stradiotto

2015

J of Separation Science

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A glassy carbon electrode chemically modified with nickel nanoparticles coupled with reversed-phase chromatography with pulsed amperometric detection was used for the quantitative analysis of furanic aldehydes in a real sample of sugarcane bagasse hydrolysate. Chromatographic separation was carried out in isocratic conditions (acetonitrile/water, 1:9) with a flow rate of 1.0 mL/min, a detection potential of -50 mV vs. Pd, and the process was completed within 4 min. The analytical curves presented limits of detection of 4.0 × 10 mol/L and 4.3 × 10 mol/L, limits of quantification of 1.3 × 10 and 1.4 × 10 mol/L, amperometric sensitivities of 2.2 × 10 nA mol/L and 2.7 × 10 nA mol/L for furfural and 5-hydroxymethylfurfural, respectively. The values obtained in this sample by the standard addition method were 1.54 ± 0.02 g/kg for 5-hydroxymethylfurfural and 11.5 ± 0.2 g/kg for furfural. The results demonstrate that this new proposed method can be used for the quick detection of furanic aldehydes without the interference of other electroactive species, besides having other remarkable merits that include excellent peak resolution, analytical repeatability, sensitivity, and accuracy.

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Section: Introductionmentioning

confidence: 99%

“…A nickel rod was employed as electrode in the detection of sugars . Sugars, sugar degradation products and alcohols were determined using a home‐made nickel electrode .…”

Section: Introductionmentioning

confidence: 99%

Determination of furanic aldehydes in sugarcane bagasse by high‐performance liquid chromatography with pulsed amperometric detection using a modified electrode with nickel nanoparticles

Silva

Beluomini

Stradiotto

2015

J of Separation Science

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“…Amperometric detection of carbohydrates at noble metal electrodes has been applied to the direct detection of carbohydrates after chromatographic separation. Various metals have been used, including gold, platinum, copper and nickel [1][2][3][4][5][6][7][8][9][10][11][12][13], in order to catalyze the electron transfer. The mechanisms and thus the detection conditions and applicability, depend upon the chemistry of the metal used.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Response of the Silver Oxide Electrode for the Detection of Carbohydrates and Related Compounds

1998

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The response of the silver oxide electrode to carbohydrates, polyhydroxy compounds and amines is dependent on several factors. These include the number of oxidizable groups on the substrate and the way in which the electrode surface is prepared, including the potential program, the thickness of the oxide layer, the surface area and the presence of other species in the solution during electrode conditioning. Studies of these factors led to a pulsed potential program for the conditioning and maintenance of electrode stability, with the incorporation of phosphate during the initial conditioning process. This method led to a response to 10 ¹4 M glucose, which was reproducible for at least forty repeated injections and stable for at least four hours with a relative standard deviation of 5.2 %. This response was also reproducible on successive days when the electrode was prepared in the same manner.

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“…Various metals have been used, including gold, platinum, copper and nickel [1][2][3][4][5][6][7][8][9][10][11][12][13], in order to catalyze the electron transfer. Various metals have been used, including gold, platinum, copper and nickel [1][2][3][4][5][6][7][8][9][10][11][12][13], in order to catalyze the electron transfer.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Response of the Silver Oxide Electrode for the Detection of Carbohydrates and Related Compounds

DeMott¹,

Tougas²,

Jahngen³

1998

Electroanalysis

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The response of the silver oxide electrode to carbohydrates, polyhydroxy compounds and amines is dependent on several factors. These include the number of oxidizable groups on the substrate and the way in which the electrode surface is prepared, including the potential program, the thickness of the oxide layer, the surface area and the presence of other species in the solution during electrode conditioning. Studies of these factors led to a pulsed potential program for the conditioning and maintenance of electrode stability, with the incorporation of phosphate during the initial conditioning process. This method led to a response to 10−4M glucose, which was reproducible for at least forty repeated injections and stable for at least four hours with a relative standard deviation of 5.2 %. This response was also reproducible on successive days when the electrode was prepared in the same manner.

show abstract

Separation of sugars by HPLC with electrochemical detection

Cited by 41 publications

References 10 publications

Determination of furanic aldehydes in sugarcane bagasse by high‐performance liquid chromatography with pulsed amperometric detection using a modified electrode with nickel nanoparticles

Determination of furanic aldehydes in sugarcane bagasse by high‐performance liquid chromatography with pulsed amperometric detection using a modified electrode with nickel nanoparticles

Optimization of Response of the Silver Oxide Electrode for the Detection of Carbohydrates and Related Compounds

Optimization of Response of the Silver Oxide Electrode for the Detection of Carbohydrates and Related Compounds

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