Separation of steroid isomers using a liquid crystalline polysiloxane stationary phase in capillary supercritical fluid chromatography

Chang, H.-C.; Markides, Karin E.; Bradshaw, Jerald S.; Lee, Milton L.

doi:10.1002/mcs.1220010305

Cited by 15 publications

(1 citation statement)

References 21 publications

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“…Liquid crystal stationary phases have been studied widely in gas chromatography (GC) and capillary SFC [4][5][6] for molecular shape recognition. The physical properties of these liquid crystal stationary phases were very similar to liquid crystals in solution because they were only surface coated on the capillary column wall.…”

Section: Introductionmentioning

confidence: 99%

Molecular shape recognition of polycyclic aromatic hydrocarbons with a liquid‐crystal bonded phase in supercritical fluid chromatography

Jinno

Mae

Saito

et al. 1991

J Microcolumn Separations

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Abstract. The chromatographic characteristics of a liquid crystal bonded phase have been evaluated for the separation of polycyclic aromatic hydrocarbons (PAHs), especially focusing on its shape recognition capability in supercritical fluid chromatography (SFC). The results clearly indicate that the phase has a stronger molecular planarity and shape recognition capability than typical polymeric and monomeric octadecylsilica (ODs) phases because of the very ordered bonded phase structure. The recognition capability was slightly dependent on SFC conditions such as column pressure, column temperature, and mobile phase density. Modifier addition did not make a large difference on the recognition capability. The phase seems to be a very promising stationary phase in SFC because the recognition capability is slightly influenced by the SFC conditions. Selectivity enhancement for molecular shape and planarity recognition has been found in SFC compared to reversed-phase LC separations with the same stationary phase.

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Section: Introductionmentioning

confidence: 99%

Molecular shape recognition of polycyclic aromatic hydrocarbons with a liquid‐crystal bonded phase in supercritical fluid chromatography

Jinno

Mae

Saito

et al. 1991

J Microcolumn Separations

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Capillary supercritical fluid chromatography of limonoids

Raynor

Roberts

Mulholland

1993

J. High Resol. Chromatogr.

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SummaryCapillary supercritical fluid chromatography (SFC) with carbon dioxide as the mobile phase has been used to separate a mixture of limonoid standards. Chromatographic conditions were optimized to enable analysis of plant extracts. The seed of Aphanamixis polysfacha (Meliaceae) and the stem bark of Harpephyllum caffrum (Anacardiaceae) were extracted with hexane and the extracts were analyzed by SFC to demonstrate the potential of the technique as a screening tool. SFC analysis indicated the presence of limonoids in the hexane extract of the bark of Entandrophragma delevoyii (Meliaceae). One limonoid and two protolimonoids isolated from the extract were separated by SFC.

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Open tubular supercritical fluid chromatography of triterpene acids from Dysoxylum pettigrewianum (Meliaceae)

Sewram

Nair

Mulholland

et al. 1995

J. High Resol. Chromatogr.

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SummaryOpen tubular supercritical fluid chromatography (SFC) with carbon dioxide as the mobile phase has been investigated for the separation of four triterpene acids, two of which were structural isomers. These compounds, isolated from Dysoxylum pettigrewiaaum (Meliaceae), were chromatographed on three stationary phases with different selectivities. 30% -biphenylmethylpolysiloxane and polyethylene glycol (Carbowax 20M) phases exhibited poor chromatographic selectivity for the compounds. The separation was improved by employing the shape selectivity of a liquid crystalline biphenylcarboxylate ester polysiloxane and utilizing simultaneous temperature and pressure programming. The elution order was explained on the basis of the molecular structures of the triterpene acids, and their interaction with the liquid crystal phase.pounds with similar chemical structures. High chromatographic efficiency and stationary phase selectivity are therefore mandatory for successful analysis.

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Separation of steroid isomers using a liquid crystalline polysiloxane stationary phase in capillary supercritical fluid chromatography

Cited by 15 publications

References 21 publications

Molecular shape recognition of polycyclic aromatic hydrocarbons with a liquid‐crystal bonded phase in supercritical fluid chromatography

Molecular shape recognition of polycyclic aromatic hydrocarbons with a liquid‐crystal bonded phase in supercritical fluid chromatography

Capillary supercritical fluid chromatography of limonoids

Open tubular supercritical fluid chromatography of triterpene acids from Dysoxylum pettigrewianum (Meliaceae)

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