Separation of Selenium-Containing Proteins in Human and Mouse Plasma Using Tandem High-Performance Liquid Chromatography Columns Coupled with Inductively Coupled Plasma-Mass Spectrometry

Selenium (Se), Blood Glutathione Peroxidases and DNA Damage in Chronic Kidney Disease Patients on Hemodialysis and After Kidney Transplantation - The Effect of Se Supplementation

“…Deagen et al (1993) have shown that the Se is incorporated, in the form of selenocysteine (Sec), to selenoprotein P (≈ 50% of plasma selenium) and GSH-Px (which accounts for 10-30% of plasma selenium) or bound in the form of selenomethionine to albumin (≈ 23%). Very similar results have been obtained for blood plasma in healthy subjects by Koyama et al (1999). They have shown a preference for supplemented Se to be taken up as selenoprotein P, the protein that incorporates up to 10 selenocysteine residues into the polypeptide chain (Persson-Moschos et al, 1995).…”

The Role of Selenium in Inflammation and Immunity: From Molecular Mechanisms to Therapeutic Opportunities

“…Of these four GPX enzymes, only GPX3 is secreted for circulation or for use in plasma, in extracellular spaces, or by neighboring cells. In fact, GPX3 accounts for 20%-40% of total plasma Se in humans (131). GPX6 is a selenoprotein in humans (in mice, Gpx6 contains cysteine (Cys) instead of Sec) that is localized to olfactory epithelium and embryonic tissues (134).…”

Isolation of Selenoprotein-P and Determination of Se Concentration Incorporated in Proteins in Human and Mouse Plasma by Tandem Heparin Affinity and Size-exclusion Column HPLC-ICPMS

“…by changing the flow rate from 0.6 to 1.0 ml min -1 and reducing the switching time of the buffer solutions from twice to once. Moreover, Koyama et al 21 did not report the quantitative results of selenoproteins in mouse plasma, although they reported the quantitative results of selenoproteins in human plasma. In this study, we established a separation system for selenoproteins in human and mouse plasma, evaluated the adsorption efficiencies of the selenoproteins, and quantified the concentrations of Se in these proteins.…”

“…Our method is a modification of a technique reported by Koyama et al 21 The mobile phase was changed from phosphate buffer to Tris-NO3 buffer, and ammonium acetate was used as an elution buffer instead of heparin. Also, the elution time was reduced from 60 to 30 min Sel-P is considered to be the most important selenoprotein for evaluating the selenium (Se) status in the body.…”