Separation of Selected Bile Acids by TLC. III. Separation on Various Stationary Phases

Separation of Selected Bile Acids by TLC. IX. Separation on Silica Gel 60 and on Silica Gel 60F254 Aluminum Plates Impregnated with Cu(II), Ni(II), Fe(II), and Mn(II) Cations

“…The R F values and separation factors (DR F and R S ) of studied bile acids separated on aluminum plates precoated with silica gel 60F 254 (#1.05554) with n -hexane-ethyl acetate -acetic acid as a mobile phase in different volume compositions (v/v/v), which were not optimal for bile acids separation on non impregnated silica gel 60F 254 at 188C [4,5] Pair of acids #1.05554) and impregnated with the respective cations: Cu(II), Ni(II), Fe(II), and Mn(II). Before use, the plates were activated at 1208C for 30 min.…”

“…[5] In the case of aluminum plates precoated with silica gel 60F 254 (#1.05554), the optimal separation of all studied bile acids at 188C was observed by using a mobile phase in volume compositions: 22:21:5 and 25:20:8 (v/v/v). [5] Moreover, it was stated that application of DiolF 254 plates facilitated the separation of examined bile acids. [6] It was proved that the temperature of 408C improves the separation of GC from GDC performed on aluminum silica gel plates (#1.05553 and #1.05554).…”

“…[3 -9] These investigations indicated that, on the non-impregnated aluminum plates precoated with silica gel 60 and silica gel 60F 254 at 188C (#1.05553 and #1.05554), the biggest problem was to separate GC from GDC and C from GLC. [5] The optimal separation of examined bile acids at 18 o C on aluminum plates precoated with silica gel 60 (#1.05553) was obtained using n -hexane -ethyl acetate -acetic acid in volume compositions: 22:21:5, 22:22:5, 25:20:8, and 20:20:5 (v/v/v). [5] In the case of aluminum plates precoated with silica gel 60F 254 (#1.05554), the optimal separation of all studied bile acids at 188C was observed by using a mobile phase in volume compositions: 22:21:5 and 25:20:8 (v/v/v).…”

“…[5] The optimal separation of examined bile acids at 18 o C on aluminum plates precoated with silica gel 60 (#1.05553) was obtained using n -hexane -ethyl acetate -acetic acid in volume compositions: 22:21:5, 22:22:5, 25:20:8, and 20:20:5 (v/v/v). [5] In the case of aluminum plates precoated with silica gel 60F 254 (#1.05554), the optimal separation of all studied bile acids at 188C was observed by using a mobile phase in volume compositions: 22:21:5 and 25:20:8 (v/v/v). [5] Moreover, it was stated that application of DiolF 254 plates facilitated the separation of examined bile acids.…”

Use of Chromatography in Animal Ecology

“…In some cases, it is chemically modified so as to provide suitable resolution conditions depending on the analyte. The correct choice of the mobile or stationary phase is essential to obtain an efficient separation of compounds to be analyzed [40][41][42]. Mobile phase generally consists of a solvent system composed of 2, 3 or 4 components which vary in their polarity and selectivity and they can include water, organic solvents and buffers [43,44].…”