Separation of pyrethrins by high-pressure liquid chromatography

Mourot, D.; Boisseau, J.; Gayot, G.

doi:10.1016/s0003-2670(01)83290-6

Cited by 12 publications

(5 citation statements)

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“…High performance liquid chromatography was used to quantitate the pyrethrins present in the cultures [9]. An Ultrasphere -Si, 5u, column (25 cm x 4.6 mm I.D.)…”

Section: Pyrethrin Analysismentioning

confidence: 99%

Selection of High Pyrethrin Producing Tissue Cultures

Sw²,

Ej³

1983

Planta Med

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Axenic callus and shoot cultures of pyrethrum have been shown to be capable of pyrethrin biosynthesis. The influence of explant source on the biosynthetic capacity in cultured tissue was examined. Explants of various plant organs were taken from high and low yielding plant selections. The conditions necessary for the establishment of these explants in culture are described. Analytical screening of the tissue lines enabled the selection of a few "high yielding" strains which were derived from high yielding plant selections. Differentiated cultures tended to produce more pyrethrins than did callus cultures.

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“…High performance liquid chromatography was used to quantitate the pyrethrins present in the cultures [9]. An Ultrasphere -Si, 5u, column (25 cm x 4.6 mm I.D.)…”

Section: Pyrethrin Analysismentioning

confidence: 99%

Selection of High Pyrethrin Producing Tissue Cultures

Sw²,

Ej³

1983

Planta Med

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“…The aim of this study was the development of a method enabling the separation of the six ester components of pyrethrum extract and the quantitative determination of the individual pyrethrins in samples of pyrethrum extract and flowers. Numerous methods for the separation and quantification of these compounds have been developed [3], including HPLC [2][3][4][5][6][7][8][9][10][11] and GC [11][12][13][14][15] methods. Because of the need for not only analytical but also preparative separation of the pyrethrins, HPLC was selected as method of choice.…”

Section: Introductionmentioning

confidence: 99%

“…Normal-phase HPLC is usually used for isolation of the individual compounds [2, 4 6, 10, 14] whereas RP chromatography is used for quantitative determination [4,[7][8][9]. The disadvantages of some of the methods are quantification by external standardization [7,10] by comparison of the peak areas of the substances with those from commercial mixtures [4,8], the need for two analyses for the determination of the six natural pyrethrins [5] or insufficient separation of the compounds [6,9]. Another problem in the analysis of the natural pyrethrins is that the individual substances are not commercially available.…”

Section: Introductionmentioning

confidence: 99%

Separation and quantitative analysis of natural pyrethrins by high-performance liquid chromatography

et al. 1999

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KeyWordsColumn liquid chromatography Pyrethrum, natural pyrethrins Chrysanthemum cinerariaefolium SummaryThe pyrethrins, six insecticidally active esters from Chrysanthemum cinerariaefolium have been separated by high-performance liquid chromatography (HPLC).A method for the separation and quantitative determination of these compounds in pyrethrum extracts by RPHPLC has been developed and validated. Quantification of the pyrethrins was performed by internal and external standardization. Normal-phase HPLC was used to isolate the individual pyrethrins. Different commercial pyrethrum extracts and samples of Flos Pyrethri of different origin were investigated by use of the new method. There was good correlation between our results and those obtained by quantification of the pyrethrins in some of the samples by the AOAC method.

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“…The analysis by high-performance liquid chromatography (HPLC) is not as difficult as GC analysis, because no degradation of the samples during the analysis has been observed. Several papers describe the analysis and successful separation of all six active compounds in pyrethrum extract. − Wintersteiger and Ofner developed the method for determining pyrethrins together with piperonyl butoxide in spiked human plasma by solid-phase extraction (SPE) followed by off- and on-line HPLC analysis. Other papers describe solid-phase extraction of pyrethrins and piperonyl butoxide of tap water or milk .…”

mentioning

confidence: 99%

“…Several papers describe the analysis and successful separation of all six active compounds in pyrethrum extract. [7][8][9][10] butoxide of tap water 12 or milk. 13 Both authors used HLPC analysis.…”

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confidence: 99%

Direct On-Line Continuous Supercritical Fluid Extraction and HPLC of Aqueous Pyrethrins Solutions

Pól

Wenclawiak

2003

Anal. Chem.

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A new method, employing supercritical fluid extraction (SFE) connected on-line with high-performance liquid chromatography, was developed for the determination of pyrethrins and piperonyl butoxide in aqueous solutions. The principle of the laboratory-made device for SFE is based on the low mutual solubility of water and supercritical (liquid) CO2. This device works in continuous mode that offers extraction of unlimited sample volumes. Different extraction temperatures and pressures were tested to find optimum extraction conditions. The addition of organic modifier and inorganic salt to the water sample to increase extraction recovery was investigated. The method was evaluated, and it was applied for the extraction of aqueous samples spiked with commercial insecticides. The working concentration range of the method was from the limit of quantification (0.1 microg L(-1)) to the solubility of the analytes in water.

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Separation of pyrethrins by high-pressure liquid chromatography

Cited by 12 publications

References 0 publications

Selection of High Pyrethrin Producing Tissue Cultures

Selection of High Pyrethrin Producing Tissue Cultures

Separation and quantitative analysis of natural pyrethrins by high-performance liquid chromatography

Direct On-Line Continuous Supercritical Fluid Extraction and HPLC of Aqueous Pyrethrins Solutions

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