Separation of porcine parvovirus from bovine serum albumin using PEG–salt aqueous two-phase system

Vijayaragavan, K. Saagar; Zahid, Amna; Young, Jonathan W.; Heldt, Caryn L.

doi:10.1016/j.jchromb.2014.07.025

Cited by 22 publications

(21 citation statements)

References 53 publications

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“…On the other hand, a decrease in the PEG chain length results in less ethylene oxide groups per PEG molecule, for the same concentration of polymer, and hence the top phase will be less hydrophobic . In this sense, Vijayaragavan et al . concluded that hydrophobicity plays a dominant role in the partitioning of porcine parvovirus to the PEG‐rich phase, which was also observed in this study.…”

Section: Resultssupporting

confidence: 82%

“…The results show that the M13 has a high top phase preference in all systems evaluated (Fig. 2, systems [17][18][19][20][21] with at least 65% of the viral particles recovered in the top phase. The total recovery yields (66.9 ± 1.5 -89.4 ± 5.1%) were higher than those obtained in PEG-salt systems.…”

Section: Partition Behavior and Recovery Of Bacteriophage M13 In Emimmentioning

confidence: 99%

“…ATPS have been exploited for several years for the recovery of bioproducts such as proteins, cells, organelles, genetic material, low‐molecular weight metabolites and viral particles . Concerning virus recovery, ATPS have been proposed for the recovery of rotavirus‐like particles, bacteriophage T4, adenovirus, porcine parvovirus, B19 parvovirus‐like particles and recombinant human immunodeficiency virus‐like particles . Application of ATPS to viruses is not common, particularly when compared with reports addressing the recovery of proteins and other types of macromolecules.…”

Section: Introductionmentioning

confidence: 99%

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Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

González‐Mora

Ruiz‐Ruiz

Benavides

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Bacteriophage M13 is an Escherichia coli‐specific non‐lytic filamentous virus commonly used in applications ranging from antibody screening and nanomaterial construction to drug delivery, among others. In this tenor, alternative methods for the fractionation, recovery and partial purification of phage particles are desired. In this work, the use of aqueous two‐phase systems (ATPS) was evaluated as an alternative method for the recovery of phage particles. RESULTS The partition behavior of M13 in PEG–salt and ionic liquid (IL)–salt ATPS was characterized using a pre‐purified feedstock. In PEG‐salt ATPS, M13 was preferentially partitioned to the interface. In IL ATPS, however, M13 showed a high‐top phase preference with recovery yields above 65%. Selected systems were tested for the extraction of M13 from a crude fermentation broth. From crude broth, a PEG 400‐potassium phosphate system with volume ratio (VR) of 1 and 25% w/w tie line length (TLL) gave the best M13 top phase recovery (83%) and purification fold (18.2) in terms of total protein concentration. CONCLUSIONS The results presented here demonstrate the practical application of ATPS as an efficient process for the primary recovery and partial purification of M13 and represent the first study of the extraction of viral particles directly from a crude broth as well as the use of IL‐Salt ATPS. © 2017 Society of Chemical Industry

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Section: Resultssupporting

confidence: 82%

Section: Partition Behavior and Recovery Of Bacteriophage M13 In Emimmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

González‐Mora

Ruiz‐Ruiz

Benavides

et al. 2017

J of Chemical Tech & Biotech

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“…The cloud point method was used to obtain the binodal curves Preparation of ATPS Assays. As previously reported in others authors' works on the literature [22,23], the ATPS assays follows a well established methodology. Previously, stocks solutions of PEG and (NH 4 ) 2 SO 4 were prepared by weighing appropriate amounts of reagents on an analytical balance (Shimadzu, AX 200 with an uncertainty of ± 0.0001 g).…”

Section: Methodsmentioning

confidence: 99%

Phase diagrams of aqueous two-phase systems formed by polyethylene glycol+ammonium sulfate+water: equilibrium data and thermodynamic modeling

Murari

Penido

Machado

et al. 2015

Fluid Phase Equilibria

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“…On the other hand, ATPS's application in the separation engineering filed, which is named aqueous two-phase extraction (ATPE), has also gained prominence. Because of the distinct advantages over the conventional organic solvent liquid-liquid extraction, such as lower cost, lower toxicity and proper interfacial tension [19], ATPE based on various ATPSs is extensively applied in chemical and biological purification and extraction, e.g., separating metal ions [20][21][22][23], purifying proteins [24][25][26], viruses [27,28] and other biological molecules [29,30]. At the same time, the boom of the discoveries of new ATPSs is responsible for the wider application of ATPE.…”

Section: Introductionmentioning

confidence: 99%

Effects of temperature and pH on phase behavior for the ternary systems of water + inorganic salt + alkoxyethanols at atmospheric pressure

Wang

et al. 2016

Journal of Molecular Liquids

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The effects of temperature, pH, inorganic salts, as well as molecular structure on the phase behavior of water + salt + alkoxyethanol(CnEm, n = 2-4, m = 1-3) ternary systems were investigated. Binodal data for water + NaCl + C2E1, C3E1, C4E1, C4E2, C4E3 were obtained and correlated to investigate how the system's phase-split ability is influenced by the type of alkoxyethanol. In addition, the binodal data for aqueous solution of C4E1 with NaCl, KCl, CsCl, NaF, NaBr were investigated to understand the effects of the salts. Furthermore, the complete two-liquid-phase coexistence regions for water + NaCl + C4E1 at T = (283.15, 293.15, 303.15, 313.15) K were acquired to investigate the effects of temperature. The liquid-liquid equilibria (LLE) were also studied based on tie-line data, in which three correlation equations, the Othmer-Tobias equation, the Bancroft equation and the Setschenow equation were used. Finally, the binodal data of water + NaCl + C4E1 was measured at pH = (3, 5, 7, 9, 11) to investigate the impact of pH.

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Separation of porcine parvovirus from bovine serum albumin using PEG–salt aqueous two-phase system

Cited by 22 publications

References 53 publications

Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

Phase diagrams of aqueous two-phase systems formed by polyethylene glycol+ammonium sulfate+water: equilibrium data and thermodynamic modeling

Effects of temperature and pH on phase behavior for the ternary systems of water + inorganic salt + alkoxyethanols at atmospheric pressure

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