Separation of Phenolic Compounds from Sugarcane Bagasse Pith and Their Determination by HPLC

Abstract

Three chromatographic techniques were compared to determine the solubilized phenolic compounds, from sugarcane bagasse pith pretreatment. Reverse phase gradient system I, was chosen as a technique to be evaluated in aromatic compounds determination, because it presented good selectivity, reproducibility and percent recovery of phenolic compounds. However, gradient system 11, and ionic exchange, resulted in longer resolution time, even though they had good resolution and selectivity. The amount of aromatic compounds determined by reverse phase gradient elution, were in decreasing order: 4-hydroxycinnamic acid (p-coumaric acid), 4-hydroxy-3-methoxycinnamic acid (ferulic acid), 4-hydroxybenzaldehyde(p-hydroxybenzaldehyde),4-hydroxy-3-methoxybenzaldehyde (vanillin), and 4-hydroxy-3-methoxybenzoic acid (vanillic acid). Reverse phase gradient system I, can be useful to identify and quantify the phenolic compounds solubilized in other lignocellulosic materials.From the experimental design 23 , the most important effect on total phenolic compounds solubilization, were: alkali concentration, temperature and alkali-moisture content interaction. In addition, the maximum amount of these compounds were obtained at high levels of experimental conditions, that is; 10% NaOH (ODW), 50 OC of temperature, and 80% of moisture content. 369

Effect of Kilning on the Antioxidant and Pro-oxidant Activities of Pale Malts

“…Release of ferulic acid, a free radical scavenger (21), from lignin and arabinoxylans during kilning at up to 80 °C also contributes to antioxidant activity in malt (6). Release is aided by hydrolytic enzymes (6) and is also affected by moisture (22); levels of ferulic acid increased in oats after steaming and drying at 100 °C (23). Yields of ferulic acid (and other phenolic acids) from malt are known to be affected by the barley, kilning conditions, and extraction solvent (4).…”

Naturally occurring phenolic sources: monomers and polymers

“…Earlier, the waste generated by agro-based industries, such as empty fruit bunches, 5 seed, 6,7 bre, shell, 8 wood and bagasse, 9 was mainly utilized either as a local source of energy by incineration 10 or as natural fertilizer. The waste is found to be rich in phenolic derivatives such as cresol, catechol, guaiacols, syringol, eugenol etc.…”

Sustainable Polymers Derived From Naturally Occurring Materials

“…Currently the agricultural wastes such as empty fruit bunches [1], seed [2][3], fibre, shell [4], wood, bagasse [5] are being investigated as a potential source of phenolic derivatives. Their main utilization at the moment is to generate energy to run the mill by incinerating the waste for power and fertilizer purposes.…”