Separation of peptides by cellulose-phosphate chromatography for identification of a hemoglobin variant

Sugihara, Jun; Imamura, Takashi; Yanase, Toshiyuki; Yamada, Hidenori; Imoto, Taiji

doi:10.1016/s0378-4347(00)86051-x

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…The use of a gel in orthopedic applications can be an interesting alternative as an injectable material, in minimally invasive surgery. Furthermore, the synthesis of anionic, phosphorus‐containing carbohydrate polymers is of interest for biomedical applications for a variety of reasons, including the preparation of peptide‐ or calcium‐binding materials, hemostatic agents, blood stabilizers, or antimicrobial surfaces 23–34, 100–102…”

Section: Resultsmentioning

confidence: 99%

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Cellulose phosphates as biomaterials. I. Synthesis and characterization of highly phosphorylated cellulose gels

Granja

Pouységu²,

Pétraud³

et al. 2001

J of Applied Polymer Sci

140

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ABSTRACT:The present study concerns the investigation of a material expected to be biocompatible and able to promote bone regeneration. For this purpose, cellulose was chemically modified by phosphorylation. Once implanted, phosphorylated cellulose could promote the formation of calcium phosphates, thus having closer resemblance to bone functionality. In a previous investigation, the obtention and the preliminary characterization of cellulose phosphate gels were reported. In the present study, the synthesis by the H 3 PO 4 /P 2 O 5 /Et 3 PO 4 /hexanol method was optimized in terms of reaction parameters. The structure of materials was investigated by FTIR, Raman, and solid-state 31 P-and 13 C-NMR spectroscopic studies, and X-ray diffraction. Water swelling and stability to sterilization by gamma-radiation were also assessed. It was demonstrated that the present method allows highly phosphorylated cellulose derivatives to be obtained. Cellulose triphosphates gels are described here for the first time. Products obtained were poorly crystallized monoesters, apparently not significantly affected by gamma sterilization and showed a high water swelling capability. Chemical bonding was confirmed by FTIR, Raman, and both 31 P-and 13 C-NMR spectroscopies. It was also shown that the H 3 PO 4 / P 2 O 5 /Et 3 PO 4 /hexanol method provides a versatile and interesting alternative route to some of the more widely used techniques for the phosphorylation of cellulose.

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Section: Resultsmentioning

confidence: 99%

“…Cellulose phosphates have been used for decades in the treatment of calcium metabolism–related diseases, taking advantage of their high ability to bind calcium ions 23–25. Several other biomedical applications have been proposed, always profiting from their high ion exchange capacity 26–34…”

Section: Introductionmentioning

confidence: 99%

Cellulose phosphates as biomaterials. I. Synthesis and characterization of highly phosphorylated cellulose gels

Granja

Pouységu²,

Pétraud³

et al. 2001

J of Applied Polymer Sci

140

View full text Add to dashboard Cite

show abstract

“…Once implanted, phosphorylated cellulose could promote the formation of calcium phosphates, thus having closer resemblance to bone functionality and ensuring a satisfactory bonding at the interface between hard tissue and the biomaterial. Furthermore, phosphate groups constitute adequate functionalities for specifically binding biologically active species,11–17 and this can be used as an advantage for obtaining specifically customized active surfaces. In a previous work, the phosphorylation reaction was carried out by an original reaction route 18, 19.…”

Section: Introductionmentioning

confidence: 99%

Cellulose phosphates as biomaterials. II. Surface chemical modification of regenerated cellulose hydrogels

Granja

Pouységu²,

Deffieux³

et al. 2001

J of Applied Polymer Sci

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ABSTRACT:Cellulose regenerated by the viscose process was previously investigated as an implantable material in orthopedic surgery. It was envisaged to take advantage not only of its good matching with mechanical properties of bone but also of its hydroexpansivity, therefore allowing a satisfactory fixation to hard tissue. Both the osteoconduction and the lack of osteoinduction of this material were demonstrated. Grafting of phosphate groups was then envisaged as the means to render cellulose more suitable for orthopedic applications by enhancing its bioactivity. In the present work, the previously optimized phosphorylation reaction was successfully adapted to the surface modification of regenerated cellulose. Modified materials were characterized by XPS, FTIR, and 31 P MAS NMR spectroscopic studies, and contact angle measurements, revealing the chemical bond between phosphate groups and cellulose, as well as the hydrophilic nature of phosphorylated materials, which increases with increasing phosphate contents. Water swelling and resistance to gamma sterilization were assessed as well, showing that phosphorylated materials swell considerably in water and were not affected when sterilization was carried out under a nitrogen atmosphere. The increase in surface roughness attributed to chemical modification was demonstrated through laser rugosimetry measurements.

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Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials

Yang

Zeng

et al. 2020

Advanced Materials

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Cellulose and hemicelluloses represent the most abundant biomaterials on the Earth and represent major structural components in cell walls of plant that provide them with sufficient mechanical Glycans play important roles in all major kingdoms of organisms, such as archea, bacteria, fungi, plants, and animals. Cellulose, the most abundant polysaccharide on the Earth, plays a predominant role for mechanical stability in plants, and finds a plethora of applications by humans. Beyond traditional use, biomedical application of cellulose becomes feasible with advances of soluble cellulose derivatives with diverse functional moieties along the backbone and modified nanocellulose with versatile functional groups on the surface due to the native features of cellulose as both cellulose chains and supramolecular ordered domains as extractable nanocellulose. With the focus on ionic cellulose-based compounds involving both these groups primarily for biomedical applications, a brief introduction about glycoscience and especially native biologically active glycosaminoglycans with specific biomedical application areas on humans is given, which inspires further development of bioactive compounds from glycans. Then, both polymeric cellulose derivatives and nanocellulose-based compounds synthesized as versatile biomaterials for a large variety of biomedical applications, such as for wound dressings, controlled release, encapsulation of cells and enzymes, and tissue engineering, are separately described, regarding the diverse routes of synthesis and the established and suggested applications for these highly interesting materials.

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Separation of peptides by cellulose-phosphate chromatography for identification of a hemoglobin variant

Cited by 7 publications

References 10 publications

Cellulose phosphates as biomaterials. I. Synthesis and characterization of highly phosphorylated cellulose gels

Cellulose phosphates as biomaterials. I. Synthesis and characterization of highly phosphorylated cellulose gels

Cellulose phosphates as biomaterials. II. Surface chemical modification of regenerated cellulose hydrogels

Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials

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