Separation of nucleosomes containing histones H1 and H5

Abstract

Hen erythrocyte chromatin was digested with staphylococcal nuclease and fractionated by electrophoresis in polyacrylamide gels. Instead of the three bands described for mouse carcinoma chromatin, four main discrete components (MN1, MN2, MN2E and MN3) were resolved in the mononucleosome fraction of erythrocyte chromatin. MN2 contained all five histones and a DNA fragment of 165--180 base pairs. MN2E comprised four nucleosomal histones plus histone H5 (but not H1) and a DNA fragment of 170--190 base pairs. The relatively nuclease resistant MN3 fraction of erythrocyte nucleosomes contained H1 but no H5 histone. A more accurate analysis of the MN2 fraction in mouse carcinoma nucleosomes revealed some additional microheterogeneity depending on the presence of two different subfractions of H1.

The organization of oligonucleosomes in yeast

“…We find two erythrocyte chromatosomes in addition to the core nucleosome, as has been reported (32). Unlike yeast, the erythrocyte core particle accumulates continuousiv relative to larger soluble nucleoproteins during the course of digestion ( Figure 4B).…”

Subnucleosomes and their relationships to the arrangement of histone binding sites along nucleosome deoxyribonucleic acid

“…Upon further digestion a third nucleosome appeared (MN3), the yields of subnucleosomes SN7, SN4, and SN1 were enhanced, and other subnucleosomes virtually disappeared. Second-dimension electrophoresis confirmed that MN2, MN1, and MNC were nucleosomes containing HI (MN2), nucleosomes containing H5 (MN1), and nucleosome cores (MNC), as described by Bakayeva & Bakayev (1978). MN3 contained core histones, HI, and a few nonhistone proteins, mainly one that remained near the top of 15% gels containing NaDodS04 (not shown).…”

Histone H1O: its location in chromatin

“…The difference in mobilities of MN2 and MN20 particles in DNP gels is of interest. This is analogous to H5-containing mononucleosomes ("MN2E") moving 22 faster than HI-containing MN2 particles in DNP gels . This could have arisen by MN2E being more negatively charged, because its DNA was 5 to 10 base pairs longer than that in MN2 , although Simpson apparently found no difference in DNA lengths between such particles.…”