Separation of nucleic acids and proteins

Harve, Rohit; Bajpai, Rakesh

doi:10.1016/s0149-6395(00)80055-0

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2004

2008

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Cited by 2 publications

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“…In the case of intracellular enzymes, the extract is heavily contaminated not only with other proteins but also with nucleic acids, so that a previous step for nucleic acid removal is customary. Several options exist, including nuclease treatment and precipitation with different agents (Harve and Bajpai 2000). Ammonium sulfate is effective in removing nucleic acids but precipitates protein as well, so more specific precipitants are required.…”

Section: Enzyme Purificationmentioning

confidence: 99%

Enzyme Production

Illanes

2008

Enzyme Biocatalysis

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Section: Enzyme Purificationmentioning

confidence: 99%

Enzyme Production

Illanes

2008

Enzyme Biocatalysis

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“…Plasmid DNA (Curling and Smiley, 2003;Harve and Bajpai, 2000;Teeters et al, 2003) exists only within cells, and here lysis is complicated by shear degradation of genomic DNA and the high effective viscosity of cell lysates. (Lehninger, 1971).…”

Section: Primary Recoverymentioning

confidence: 99%

Bioseparations

Lightfoot

Moscariello

2004

Biotech & Bioengineering

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Here we review key applications of separation technology in applied biology. We first sketch out the field as a whole, but then narrow our scope to the processing of fermentation products, particularly to high-value biologicals such as proteins and nucleotides. We go on to provide a qualitative overview describing the importance and general nature of this large field, major trends, and the strategies that have proven most fruitful in evolving effective separation and purification processes. We then give a detailed description of individual separations equipment and the principles governing their operation. We concentrate throughout on making the available literature accessible to the reader; we provide what is hoped to be a representative set of basic references. However, these references, in turn, include some that suggest promising new developments as well as a number of more specialized reviews. We hope that our overall result provides the reader with access to the most relevant literature. B

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Separation of nucleic acids and proteins

Cited by 2 publications

References 57 publications

Enzyme Production

Enzyme Production

Bioseparations

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