Separation of nine compounds fromSalvia plebeiaR.Br. using two-step high-speed counter-current chromatography with different elution modes

Abstract

Nine compounds were successfully separated from Salvia plebeia R.Br. using two-step high-speed counter-current chromatography with three elution modes. Elution-extrusion counter-current chromatography was applied in the first step, while classical counter-current chromatography and recycling counter-current chromatography were used in the second step. Three solvent systems, n-hexane/ethyl acetate/ethanol/water (4:6.5:3:7, v/v), methyl tert-butyl ether/ethyl acetate/n-butanol/methanol/water (6:4:1:2:8, v/v) and n-hexane/ethyl acetate/methanol/water (5:5.5:5:5, v/v) were screened and optimized for the two-step separation. The separation yielded nine compounds, including caffeic acid (1), 6-hydroxyluteuolin-7-glucoside (2), 5,7,3',4'-tetrahydroxy-6-methoxyflavanone-7-glucoside (3), nepitrin (4), rosmarinic acid (5), homoplantaginin (6), nepetin (7), hispidulin (8), and 5,6,7,4'-tertrahydroxyflavone (9). To the best of our knowledge, 5,7,3',4'-tetrahydroxy-6-methoxyflavanone-7-glucoside and 5,6,7,4'-tertrahydroxyflavone have been separated from Salvia plebeia R.Br. for the first time. The purities and structures of these compounds were identified by high-performance liquid chromatography, electrospray ionization mass spectrometry, (1)H and (13)C NMR spectroscopy. This study demonstrates that high-speed counter-current chromatography is a useful and flexible tool for the separation of components from a complex sample.

Medicinal importance, pharmacological activities, and analytical aspects of hispidulin: A concise report

“…Elution–extrusion counter-current chromatography was applied in the first step, while classical counter-current chromatography and recycling counter-current chromatography were used in the second step. The separation yielded nine compounds including hispidulin in the Salvia plebeian extract 39 . High-speed countercurrent chromatography (HSCCC) was successfully applied for the isolation and purification of flavonoids from Herba salviae Plbeiae using stepwise and dual-mode elution with a pair of two-phase solvent systems.…”

“…Bioactivity-guided fractionation of the methanol extract of the leaves of Santolina insularis led to the isolation of hispidulin and other xanthone 39 . Seven flavonoidal compounds including hispidulin were isolated from the flowers of C. rivulare (Jacq.)…”

Separation of six compounds from pigeon pea leaves by elution–extrusion counter‐current chromatography

“…Hence the EECCC mode was adopted for efficient separation. For EECCC mode, the suitable K value range could be elongated to 0.4-16 [25]. The α values in n-hexane/methanol/formic acid solution system with solvent volume ratio of 10:4:6 (pH = 3), 10:6:4 (pH = 3), 10:7:3 (pH = 2) and 10:5:5 (pH = 1) were all larger than 1.5 as shown in Table 1.…”

New Eudesmane Sesquiterpenoids from Salvia plebeia R. Br.

“…Phytochemical studies of S . plebeia revealed the presence of terpenoids, flavonoids and lignans in the plant. Here we describe the isolation and structure elucidation of three new endesmane sesquiterpenoids, named salplebeones A – C ( 1 – 3 ).…”

“…[3 -6] Salvia plebeia R. BR has traditionally been used as folk medicine for its hemogenetic, hemostatic, antioncotic, and anti-inflammatory effects. [7] Phytochemical studies of S. plebeia revealed the presence of terpenoids, [2][8] [9] flavonoids [10] and lignans [11] in the plant. Here we describe the isolation and structure elucidation of three new endesmane sesquiterpenoids, named salplebeones A -C (1 -3).…”