Separation of lymphocytes using acoustic microfluidics

Kotz, Kenneth T.; Dubay, Ryan; Berlin, Dean Larios; Fiering, Jason

doi:10.1016/j.jcyt.2017.02.035

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, advancements have been made in separating RBCs and WBCs using acoustofluidics. In 2017, Kotz et al 104 reported the enrichment of lymphocytes with 55% recovery rate and 90% RBC depletion ratio by using BAW technique and tuning the diluent. Urbansky et al 105 reported that by changing the buffer conditions with different percentage of Stock Isotonic Percoll solution, the separation of WBCs and RBCs was successfully achieved in a two-stage acoustofluidic separation device, as shown in Fig.…”

Section: Separation Of Blood Componentsmentioning

confidence: 99%

Acoustofluidic separation of cells and particles

et al. 2019

View full text Add to dashboard Cite

Acoustofluidics, the integration of acoustics and microfluidics, is a rapidly growing research field that is addressing challenges in biology, medicine, chemistry, engineering, and physics. In particular, acoustofluidic separation of biological targets from complex fluids has proven to be a powerful tool due to the label-free, biocompatible, and contact-free nature of the technology. By carefully designing and tuning the applied acoustic field, cells and other bioparticles can be isolated with high yield, purity, and biocompatibility. Recent advances in acoustofluidics, such as the development of automated, point-of-care devices for isolating sub-micron bioparticles, address many of the limitations of conventional separation tools. More importantly, advances in the research lab are quickly being adopted to solve clinical problems. In this review article, we discuss working principles of acoustofluidic separation, compare different approaches of acoustofluidic separation, and provide a synopsis of how it is being applied in both traditional applications, such as blood component separation, cell washing, and fluorescence activated cell sorting, as well as emerging applications, including circulating tumor cell and exosome isolation.

show abstract

Section: Separation Of Blood Componentsmentioning

confidence: 99%

Acoustofluidic separation of cells and particles

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Microfluidic cell separation by acoustic methods has been described in the research literature for more than a decade, 11 but it has only recently reached the degree of maturity that it could be envisioned for clinical bioprocessing. 12 As we show here, acoustic separation has advantages because (1) it depletes monocytes and neutrophils more effectively than centrifugation; (2) the microchannel format results in very low internal volume, such that sample loss is negligible; and (3) it is a continuous-flow method, which can be adjusted to sample volume, and in the future could be coupled directly to other flow-through modules in an end-to-end processing system. If these advantages can be translated into a manufacturable and reliable instrument, acoustic separation could replace current methods to purify blood products and offer a high degree of automation, scalability, and output purity, which could improve process control and efficiency in cell therapy.…”

Section: Introductionmentioning

confidence: 94%

Purification of Lymphocytes by Acoustic Separation in Plastic Microchannels

et al. 2018

View full text Add to dashboard Cite

Emerging cell therapies have created new demands for instruments that will increase processing efficiency. Purification of lymphocytes prior to downstream steps of gene transfer currently relies on centrifugal separation, which has drawbacks in output sample purity and process automation. Here, we present an alternative approach to blood cell purification using acoustic forces in plastic microchannels. We provide details regarding the system's ability to purify lymphocytes relative to other blood cell types while maintaining a high overall recovery, testing performance starting from leukapheresis product, buffy coat, and whole blood. Depending on settings, the device achieves for lymphocytes up to 97% purity and up to 68% recovery, and depletes 98% of monocytes while also reducing red cells and platelets. We expect that future scale-up of our system for increased throughput will enable its incorporation in the cell therapy workflow, and that it could ultimately reduce costs and expand access for patients.

show abstract

“…To apply acoustic waves into the microfluidic devices, piezoelectric transducers that can generate mechanical deformation using electrical polarization are prepared near the microfluidic separation channels. Many types of wave generators exist depending on the characteristics of piezoelectric materials, electrode design, and application pattern of electric fields [ 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 ]. Basically, an alternating current (AC) signal is applied to the planar piezoelectric transducer that vibrates at the frequency of the AC signal, creating an acoustic wave.…”

Section: Active Separation Group 1: Non-contacting Mechanical Forcesmentioning

confidence: 99%

Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

Choe

Kim

2021

Biosensors

View full text Add to dashboard Cite

Separation of micro- and nano-sized biological particles, such as cells, proteins, and nucleotides, is at the heart of most biochemical sensing/analysis, including in vitro biosensing, diagnostics, drug development, proteomics, and genomics. However, most of the conventional particle separation techniques are based on membrane filtration techniques, whose efficiency is limited by membrane characteristics, such as pore size, porosity, surface charge density, or biocompatibility, which results in a reduction in the separation efficiency of bioparticles of various sizes and types. In addition, since other conventional separation methods, such as centrifugation, chromatography, and precipitation, are difficult to perform in a continuous manner, requiring multiple preparation steps with a relatively large minimum sample volume is necessary for stable bioprocessing. Recently, microfluidic engineering enables more efficient separation in a continuous flow with rapid processing of small volumes of rare biological samples, such as DNA, proteins, viruses, exosomes, and even cells. In this paper, we present a comprehensive review of the recent advances in microfluidic separation of micro-/nano-sized bioparticles by summarizing the physical principles behind the separation system and practical examples of biomedical applications.

show abstract

Separation of lymphocytes using acoustic microfluidics

Cited by 4 publications

References 0 publications

Acoustofluidic separation of cells and particles

Acoustofluidic separation of cells and particles

Purification of Lymphocytes by Acoustic Separation in Plastic Microchannels

Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

Contact Info

Product

Resources

About