Separation of lac dye components by high-speed counter-current chromatography

Oka, Hisao; Ito, Yûko; Yamada, Sadaji; Kagami, Tadaaki; Hayakawa, Junko; Harada, Kenichi; Atsumi, Eiichiro; Suzuki, Masanao; Suzuki, Makoto; Odani, Hiroko; Akahori, Setsuko; Maeda, Kenji; Nakazawa, Hiroyuki; Ito, Yoichiro

doi:10.1016/s0021-9673(98)00311-2

Cited by 42 publications

(25 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The following LC conditions were used: column, XTerra MS C18 column 14 N-and 15 N-4-Aminolaccaic acid C (3 and 3 ) were synthesized by heating laccaic acid C (3) in 14 N-or 15 Nammonia solution, respectively, and then the samples were subjected to preparative LC/MS using the condi- …”

Section: Preparation Of Ammonia-treated Lac Dye For Lc/ms Analysismentioning

confidence: 99%

Preparation of a Ammonia-Treated Lac Dye and Structure Elucidation of Its Main Component

Nishizaki¹,

Ishizuki²,

Akiyama³

et al. 2016

Journal of the Food Hygienics Society of Japan

View full text Add to dashboard Cite

Lac dye and cochineal extract contain laccaic acids and carminic acid as the main pigments, respectively. Both laccaic acids and carminic acid are anthraquinone derivatives. 4-Aminocarminic acid (acid-stable carmine), an illegal colorant, has been detected in several processed foods. 4-Aminocarminic acid is obtained by heating cochineal extract (carminic acid) in ammonia solution. We attempted to prepare ammonia-treated lac dye and to identify the structures of the main pigment components. Ammonia-treated lac dye showed acid stability similar to that of 4-aminocarminic acid. The structures of the main pigments in ammonia-treated lac dye were analyzed using LC/MS. One of the main pigments was isolated and identified as 4-aminolaccaic acid C using various NMR techniques, including 2D-INADEQUATE. These results indicated that ammonia-treatment of lac dye results in the generation of 4-aminolaccaic acids.

show abstract

Section: Preparation Of Ammonia-treated Lac Dye For Lc/ms Analysismentioning

confidence: 99%

Preparation of a Ammonia-Treated Lac Dye and Structure Elucidation of Its Main Component

Nishizaki¹,

Ishizuki²,

Akiyama³

et al. 2016

Journal of the Food Hygienics Society of Japan

View full text Add to dashboard Cite

show abstract

“…Similar observations (registration of pseudomolecular ions and those attributed to fragments without H 2 O or CO 2 in the mass spectra of laccaic acids) have also been reported previously. 41 The identification of laccaic acids A and E allowed us to conclude that the main component of the examined lac-dye preparation was manufactured in India. The content of the laccaic acid A in natural dyestuff estimated by use of the MS method was 72 š 12% (w/w).…”

Section: Identification Of Components Of Natural Dyestuffs Of Animal mentioning

confidence: 99%

Identification of anthraquinone coloring matters in natural red dyes by electrospray mass spectrometry coupled to capillary electrophoresis

et al. 2003

View full text Add to dashboard Cite

Capillary electrophoresis with UV/visible diode-array detection (DAD) and electrospray mass spectrometric (ESI-MS) detection were used for the identification of anthraquinone color components of cochineal, lac-dye and madder, natural red dyestuffs often used by ancient painters. For the purpose of such analysis, ESI-MS was found to be a much more appropriate detection technique than DAD one owing to its higher sensitivity (detection limits in the range 0.1-0.5 micro g ml(-1)) and selectivity. The method developed made it possible to identify unequivocally carminic acid and laccaic acids A, B and E as coloring matters in the examined preparations of cochineal and lac-dye, respectively. In madder, European Rubia tinctorum, alizarin and purpurin were found. The method allows the rapid, direct and straightforward identification and quantification of components of natural products used in art and could be very helpful in restoration and conservation procedures.

show abstract

“…Lac dye is used extensively as a natural food additive [9,10], and in cosmetics [9], as well as a colourant for silk and cotton dyeings [11]. In the north and northeast of Thailand, lac dye is a natural red dyestuff extracted for cotton and silk dyeings [11].…”

Section: Introductionmentioning

confidence: 99%

An adsorption and thermodynamic study of lac dyeing on cotton pretreated with chitosan

Rattanaphani¹,

Chairat²,

Bremner³

et al. 2007

Dyes and Pigments

145

View full text Add to dashboard Cite

Separation of lac dye components by high-speed counter-current chromatography

Cited by 42 publications

References 15 publications

Preparation of a Ammonia-Treated Lac Dye and Structure Elucidation of Its Main Component

Preparation of a Ammonia-Treated Lac Dye and Structure Elucidation of Its Main Component

Identification of anthraquinone coloring matters in natural red dyes by electrospray mass spectrometry coupled to capillary electrophoresis

An adsorption and thermodynamic study of lac dyeing on cotton pretreated with chitosan

Contact Info

Product

Resources

About