Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography

Vorbeck, Marie L.; Marinetti, G.V.

doi:10.1016/s0022-2275(20)39630-9

Cited by 236 publications

(23 citation statements)

References 16 publications

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“…The yield of lipid from all three strains was 4 to 5% dry cell weight. Each lipid extract was separated into neutral, glycolipid, and phospholipid fractions by silicic acid chromatography (21). The neutral fractions which were devoid of carbohydrate and phosphorus were not investigated further.…”

Section: Methodsmentioning

confidence: 99%

Lipid Composition of Some Species of Arthrobacter

Shaw

Stead

1971

J Bacteriol

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The lipids from Arthrobacter crystallopoietes, A. pascens , and A. globiformis were investigated. Each strain contained three glycolipids, a monogalactosyl diglyceride, a digalactosyl diglyceride, and a dimannosyl diglyceride, and traces of triand tetraglycosyl diglycerides. The phospholipids in all three strains consisted of bisphosphatidylglycerol, phosphatidylglycerol, and phosphatidyl myo inositol. No evidence could be obtained for the occurrence of mannophosphoinositides. Analysis of the fatty acids by gas-liquid chromatography showed that they are predominantly C 15:0 anteiso and C 17:0 anteiso compounds. No significant differences were observed in the composition of lipids extracted from homogeneous cell preparations of the rod and sphere forms of A. crystallopoietes .

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Section: Methodsmentioning

confidence: 99%

Lipid Composition of Some Species of Arthrobacter

Shaw

Stead

1971

J Bacteriol

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“…3A. Peak E was rechro)matographed on a Un7isil column (35,50). The loaded column was first eluted with chloroform, theiz acetonte, aiid finally methantolacetic acid (100:1, v/v).…”

Section: Lysis Of M Pneumoniae By Hypotonic Shockmentioning

confidence: 99%

“…Total lipid samples were fractionated by silicic acid column chromatography. To separate low polarity (neutral), intermediate polarity (glycolipid), and polar (phosphatide) lipids, a Unisil column technique (35,50), which was modified to incorporate 1% (v/v) acetic acid in the methanol solvent, was used. Alternatively, a modification of the column procedures of Hirsch and Ahrens (8) was used to resolve neutral lipids from each other and from a fraction containing both glycolipids and phosphatides.…”

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confidence: 99%

Isolation, Characterization, and Immunogenicity of Mycoplasma pneumoniae Membranes

1970

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Membrane and soluble fractions of Mycoplasma pneumoniae, M. pulmonis , and M. laidlawii B were prepared by hypotonic lysis of whole cells. The membranes of M. pneumoniae and M. laidlawii B contained, as percentage of dry weight: 34 to 37% protein, 59 to 61% lipid, 3 to 4% carbohydrate as hexose, and 0.2% ribonucleic acid as ribose. NADH 2 and NADPH 2 oxidase activities were localized in the soluble fractions of M. pneumoniae and in the membrane fraction of M. laidlawii B. NADH 2 oxidase activity was localized in the soluble fraction of M. pulmonis . The lipids of M. pneumoniae were labeled when the organism was grown in the presence of either radioactive palmitic acid, oleic acid, cholesterol, or glycerol. The lipids were not labeled when grown in the presence of radioactive acetate. Palmitic acid radio-activity was found in neutral lipid, glycolipid, and phosphatide fractions. Immunodiffusion analyses of whole cells and membrane fractions demonstrated three reactive antigens. Two immunodiffusion antigens were localized in the membrane fraction. One of these apparently contains lipid. A third antigen, also considered lipoidal, was found in whole cells. Membrane and soluble fractions of M. pneumoniae were immunogenic. The immunogens eliciting metabolic-inhibiting antibodies were localized in the membrane. The membrane preparation also induced the formation of antibodies which fixed complement with an antigen extracted with lipid solvent. The soluble fraction contained a distinct immunogen which induces antibodies reactive in complement fixation with an antigen prepared by phenol extraction.

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“…When the total lipid extract was applied on a silicic acid column, 25 % of the total amount of lipid, consisting of apolar lipids, was eluted with chloroform. A fraction containing 26% of the total lipids was obtained by eluting the column with increasing concentrations of acetone in chloroform (49). No phosphorus could be detected in this fraction which corresponds probably to glycolipid.…”

Section: Resultsmentioning

confidence: 99%

Phospholipids of Streptococcus faecalis

Mota¹,

Kamp²,

Verheij³

et al. 1970

J Bacteriol

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Autoradiograms of total lipid extracts from Streptococcus faecalis ATCC 9790, harvested in the stationary phase from a medium containing 32 P-orthophosphate, showed six major spots. The corresponding compounds were identified as diphosphatidylglycerol (possibly with a penta acyl structure); phosphatidylglycerol; a provisionally identified mixture of alanylphosphatidylglycerol and of the 2′-lysyl-derivative of phosphatidylglycerol; the 3′-lysyl-derivative of phosphatidylglycerol, probably together with some arginylphosphatidylglycerol; a diglucosyl derivative of phosphatidylglycerol; and a compound which was tentatively identified as the 2′,3′-dilysyl derivative of phosphatidylglycerol.

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Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography

Cited by 236 publications

References 16 publications

Lipid Composition of Some Species of Arthrobacter

Lipid Composition of Some Species of Arthrobacter

Isolation, Characterization, and Immunogenicity of Mycoplasma pneumoniae Membranes

Phospholipids of Streptococcus faecalis

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