Separation of Glucose-1-Phosphate and Glucose-6-Phosphate by Paper Chromatography

Harrap, F. E. G.

doi:10.1038/182876a0

Nature

1958

DOI: 10.1038/182876a0

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Separation of Glucose-1-Phosphate and Glucose-6-Phosphate by Paper Chromatography

F. E. G. Harrap¹

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1961

1980

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Cited by 12 publications

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Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography

Blake

Goldstein

1980

Analytical Biochemistry

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Bandeiraea simplicifolia I (BS I) isolectins, immobilized on Sepharose beads, specifically retarded low molecular weight ligands containing terminal cu-o-galactopyranosyl and 2acetamido-2-deoxy-aD -galactopyranosyl residues. A BS I lectin-Sepharose column has been used to perform very efficient separations of structurally homologous sugar nucleotides and oligosaccharides. For example, UDP-glucose and UDP-galactose (and the corresponding 2-acetamido-2-deoxy derivatives) were separated by as much as three column volumes by a BS I lectin-Sepharose column. This column has also been used to resolve the alditols of lactose (P-o-Galp-(1 4 4)-D-Gk) and melibiose (a-o-Galp-(1 + 6)-~431~). to analyze the radiochemical purity of UDP-galactose and UDP-N-acetyl-D-galactosamine preparations, and to analyze the relative proportions of UDP-N-acetyl-D-glucosamine and UDP-N-acetyl-o-galactosamine in the UDP-N-acetyl-o-hexosamine pool isolated from cultured cells.

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Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography

Blake

Goldstein

1980

Analytical Biochemistry

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Galactose-sensitive mutants of Salmonella I. Metabolism of galactose

Nikaido

1961

Biochimica et Biophysica Acta

137

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The separation and identification of oligosaccharides

Bailey

Pridham

1962

Chromatographic Reviews

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Separation of Glucose-1-Phosphate and Glucose-6-Phosphate by Paper Chromatography

Cited by 12 publications

References 4 publications

Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography

Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography

Galactose-sensitive mutants of Salmonella I. Metabolism of galactose

The separation and identification of oligosaccharides

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