Separation of Globin Chains by Rapid Cellulose Acetate Electrophoresis

Komarmy, Louis; Barnes, Marshall G.

doi:10.1093/ajcp/58.5.428

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1974

1979

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Cited by 6 publications

(2 citation statements)

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“…Electrophoresis of the globin chains was carried out as described by Komarmy and Barnes [17], the thermal stability kinetic pattern as described by Wajcman et at. [24], the isopropanol test as described by C arrel and Kay [6], the glycerol lysis time as described by G ottfried and Robertson [15].…”

Section: Methodsmentioning

confidence: 99%

Haemoglobin Lepore Boston in a Spanish Family

E¹,

Baiget²,

Darbre³

et al. 1976

Acta Haematol

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Section: Methodsmentioning

confidence: 99%

Haemoglobin Lepore Boston in a Spanish Family

E¹,

Baiget²,

Darbre³

et al. 1976

Acta Haematol

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“…The abnormal haemoglobin was characterized in 40 specimens (one specimen from each family) by separation of globin chains on cellulose acetate (Komarmy and Barnes, 1972) and by hybridization with canine haemoglobin (Huehns et al, 1962). Globin was prepared by the method of Rossi-Fanelli et al (1958), separation of the polypeptide chains and tryptic digestion was carried out as described by Clegg et al (1966), the fingerprints were obtained according to the method of Ingram (1958) as modified by Baglioni (1961).…”

mentioning

confidence: 99%

Haemoglobin Hasharon in a north Italian community.

Alberti¹,

Mariuzzi²,

Marinucci³

et al. 1975

Journal of Medical Genetics

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Interaction of ?-thalassemia genes with each other and with HbC in an American black family

1979

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The relative rates of in vitro synthesis of hemoglobin chains have been studied in an American black family in which the mother is doubly heterozygous for alpha-thalassemia and HbC and the father is heterozygous for alpha-thalassemia. The alpha/non-alpha synthetic ratio was equally unbalanced in both the bone marrow and the peripheral blood of the mother. Although HbC comprised 35% of her hemoglobin (compared to 42.2 +/- 2.2 in individuals with HbC trait and balanced globin synthesis), synthetic data showed that the newly synthesized beta C chain was 44% of the total newly synthesized beta chains. Isolated membranes contained more newly synthesized beta C than beta A chains. Three of the offspring were within the normal range, and the remaining three had alpha-thalassemia. There were two spontaneous abortions during the second trimester of pregnancy. Hydrops fetalis did not occur, and none of the children had HbH disease or HbC trait.

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Separation of Globin Chains by Rapid Cellulose Acetate Electrophoresis

Cited by 6 publications

References 0 publications

Haemoglobin Lepore Boston in a Spanish Family

Haemoglobin Lepore Boston in a Spanish Family

Haemoglobin Hasharon in a north Italian community.

Interaction of ?-thalassemia genes with each other and with HbC in an American black family

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