Separation of gamma-aminobutyric acid from fermented broth

Li, Haixing; Qiu, Ting; Chen, Yan

doi:10.1007/s10295-011-0984-x

Cited by 16 publications

(10 citation statements)

References 30 publications

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“…In addition, the harshness of the environment was aggravated by the additional Na 2 SO 4 , which was harmful to the strain and inhibited GABA synthesis. Moreover, the produced by-product Na 2 SO 4 increased the difficulty of purifying the end product [ 26 , 32 ]. Compared to MSG, l -glutamic acid has much lower solubility, and the substitution of H + for a Na 2+ on the α-carboxyl group confers l -glutamic acid the properties of sustained release and pH buffering.…”

Section: Resultsmentioning

confidence: 99%

Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912

Wang

Liu

et al. 2018

Microb Cell Fact

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BackgroundGamma-aminobutyric acid (GABA) plays a significant role in the food and drug industries. Our previous study established an efficient fed-batch fermentation process for Lactobacillus brevis NCL912 production of GABA from monosodium l-glutamate; however, monosodium l-glutamate may not be an ideal substrate, as it can result in the rapid increase of pH due to decarboxylation. Thus, in this study, l-glutamic acid was proposed as a substrate. To evaluate its potential, key components of the fermentation medium affecting GABA synthesis were re-screened and re-optimized to enhance GABA production from L. brevis NCL912.ResultsThe initial fermentation medium (pH 3.3) used for optimization was: 50 g/L glucose, 25 g/L yeast extract, 10 mg/L manganese sulfate (MnSO4·H2O), 2 g/L Tween-80, and 220 g/L l-glutamic acid. Glucose, a nitrogen source, magnesium, and Tween-80 had notable effects on GABA production from the l-glutamic acid-based process; other factors showed no or marginal effects. The optimized levels of the four key components in the fermentation medium were 25 g/L glucose, 25 g/L yeast extract FM408, 25 mg/L MnSO4·H2O, and 2 g/L Tween-80. A simple and efficient fermentation process for the bioconversion of GABA by L. brevis NCL912 was subsequently developed in a 10 L fermenter as follows: fermentation medium, 5 L; glutamic acid, 295 g/L; inoculum, 10% (v/v); incubation temperature, 32 °C; and agitation, 100 rpm. After 48 h of fermentation, the final GABA concentration increased up to 205.8 ± 8.0 g/L.Conclusionsl-Glutamic acid was superior to monosodium l-glutamate as a substrate in the bioproduction of GABA. Thus, a high efficacy bioprocess with 205 g/L GABA for L. brevis NCL912 was established. This strategy may provide an alternative for increasing the bioconversion of GABA.

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Section: Resultsmentioning

confidence: 99%

Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912

Wang

Liu

et al. 2018

Microb Cell Fact

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“…The GABA fermentation medium was composed of (g L −1 ): glucose, 35; soya peptone, 25; l ‐glutamate, 400 mM; MnSO 4 ·4H 2 O, 0.01; and Tween 80, 2 mL L −1 ; pH 5.0. Seed culture preparation and fed‐batch fermentation of L. brevis NCL912 were carried out as previously described (Li et al ., , ) and samples were withdrawn every 12 h for up to 48 h after beginning the fermentation experiment. Escherichia coli DH5α was routinely grown at 37 °C in Luria–Bertani (LB) medium; E. coli DH5α cells containing recombinant DNA plasmids were grown in LB medium supplemented with ampicillin (100 μg mL −1 ) at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

gadAgene locus inLactobacillus brevisNCL912 and its expression during fed-batch fermentation

Liu

2013

FEMS Microbiol Lett

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Normally, Lactobacillus brevis has two glutamate decarboxylase (GAD) genes; gadA and gadB. Using PCR, we cloned the gadA gene from L. brevis strain NCL912, a high yield strain for the production of gamma-aminobutyric acid (GABA). However, despite using 61 different primer pairs, including degenerate primers from conserved regions, we were unable to use PCR to clone gadB from the NCL912 strain. Furthermore, we could not clone it by genomic walking over 3000 bp downstream of the aldo-keto reductase gene, a single-copy gene that is located 1003 bp upstream of gadB in L. brevis ATCC367. Altogether, the data suggest that L. brevis NCL912 does not contain a gadB gene. By genomic walking, we cloned regions upstream and downstream of the gadA gene to obtain a 4615 bp DNA fragment that included the complete gadA locus. The locus contained the GAD gene (gadA) and the glutamate:GABA antiporter gene (gadC), which appear to be transcribed in an operon (gadCA), and a transcriptional regulator (gadR) of gadCA. During whole fed-batch fermentation, the expression of gadR, gadC and gadA was synchronized and correlated well with GABA production. The gadA locus we cloned from NCL912 has reduced homology compared with gadA loci of other L. brevis strains, and these differences might explain the ability of NCL912 to produce higher levels of GABA in culture.

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“…Only Park et al . mentioned its separation through an ion exchange column, similar to their previous reports for separation of γ‐aminobutyrate (GABA), yet their process was conducted by successive centrifugation, filtration, decoloration, desalination and ion‐exchange chromatography . Among them, desalination must be carried out before ion exchange due to a large amount of buffered salts present in the bioconversion liquid, resulting in the separation of GABA from its fermentation broth becoming a time‐consuming and costly process.…”

Section: Introductionmentioning

confidence: 92%

Separation of 5‐aminovalerate from its bioconversion liquid by macroporous adsorption resin: mechanism and dynamic separation

Lü

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: 5-aminovalerate (5-AVA) is the precursor of valerolactam, a potential building block for producing nylon 5, and also regarded as a potential C5 chemical platform. Although 5-AVA bioproduction has been developed, few studies have emphasized the separation of 5-AVA from the bioconversion liquid.RESULTS: An effective and benign method for separation of 5-AVA from its bioconversion liquid was proposed based on chromatography technology using macroporous adsorption resin AK-1. This work focuses on explosion of the adsorption mechanism and optimization of the operating conditions. Freundlich model and thermodynamics results showed that the adsorption affinity of 5-AVA to the AK-1 resin was weaker than that of L-lysine. Combination of the characterization results of AK-1 resin with the physicochemical properties of 5-AVA and L-lysine, the adsorption mechanism was proposed as meso-micropore diffusion, and hydrogen bonding and hydrophobic effects. In dynamic studies, a 5-AVA purity of 99.3% and yield of 92% was obtained at an operating temperature of 353.15 K on a 40:1 chromatographic column eluted by deionized water. The residual 5-AVA and L-lysine were recovered by 1 mol L -1 HCl, which can be used as a feed material in the 5-AVA conversion process repeatedly.CONCLUSION: An innovative benign efficient environmentally process for recovery of 5-AVA from the bioconversion liquid was developed based on a macroporous resin AK-1. Compared with ion exchange resins, macroporous resins can efficiently adsorb 5-AVA, whereas salt ions cannot be retained. This clean and efficient method provides a promising strategy for separating similar biochemical products.

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Separation of gamma-aminobutyric acid from fermented broth

Cited by 16 publications

References 30 publications

Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912

Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912

gadAgene locus inLactobacillus brevisNCL912 and its expression during fed-batch fermentation

Separation of 5‐aminovalerate from its bioconversion liquid by macroporous adsorption resin: mechanism and dynamic separation

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