Separation of Flavonoids from the Leaves of Oroxylum indicum by HSCCC

Yuan, Yuan; Hou, Wen-Li; Tang, Mingli; Luo, Houding; Chen, Lijuan; Guan, Yue; Sutherland, Ian

doi:10.1365/s10337-008-0859-0

Cited by 51 publications

(37 citation statements)

References 17 publications

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“…Their structures were confirmed after comparing their spectral data with that found in the literature (Facundo & Morais, 2003;Park et al, 2007;Yuan et al, 2008). To the best of our knowledge, the effect of antimicrobial compounds from the hybrid poplar 'Neva' on plant pathogens have not yet been reported.…”

Section: Introductionmentioning

confidence: 58%

Antimicrobial flavonoids from the twigs ofPopulus nigra × Populus deltoides

Zhong

Zhou

et al. 2012

Natural Product Research

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A bioassay-guided fractionation of the ethyl acetate extract from the twigs of the hybrid poplar 'Neva', Populus nigra L. × Populus deltoides Marsh, led to the isolation of three flavonoids, which were identified by means of spectrometric and physicochemical analysis as 5-hydroxy-7-methoxy-flavone (1), 5,7-dihydoxy-flavone (2) and 5,7-dihydroxy-flavonol (3). These compounds were further screened for their antimicrobial activity against plant pathogens, including three bacteria (Pseudomonas lachrymans, Ralstonia solanacearum and Xanthomonas vesicatoria) and one fungus (Magnaporthe oryzae). Compounds 2 and 3 showed significant antibacterial activity, with minimum inhibitory concentrations (MICs) ranging from 15 to 25 µg mL(-1), and median inhibitory concentrations (IC(50) values) from 4 to 18 µg mL(-1). The results obtained provide promising baseline information for the potential use of the extract and flavonoids from this plant as antimicrobial agents to help control plant diseases.

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Section: Introductionmentioning

confidence: 58%

Antimicrobial flavonoids from the twigs ofPopulus nigra × Populus deltoides

Zhong

Zhou

et al. 2012

Natural Product Research

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“…Furthermore, it is suitable to industrial production. For example, an HSCCC system has been employed to separate seven flavonoids from a methanolic extract of the leaves of Oroxylum indicum by a one-step isocratic elution using a chloroform-methanol-water (9.5:10.5) two-phase system [25].…”

Section: High-speed Counter Current Chromatography (Hsccc)mentioning

confidence: 99%

Isolation and Structure Identification of Flavonoids

Feng¹,

Hao²,

Li³

2017

Flavonoids - From Biosynthesis to Human Health

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Flavonoids, which possess a basic C15 phenyl-benzopyrone skeleton, refer to a series of compounds in which two benzene rings (ring A and B) are connected to each other through three carbon atoms. Based on their core structure, flavonoids can be grouped into different flavonoid classes, such as flavonols, flavones, flavanones, flavanonols, anthocyanidins, isoflavones and chalcones. Flavonoids are often hydroxylated in positions 3, 5, 7, 3 0 ,4 0 and/or 5 0 . Frequently, one or more of these hydroxyl groups are methylated, acetylated, prenylated or sulfated. In plants, flavonoids are often present as O-or C-glycosides. The O-glycosides have sugar substituents bound to a hydroxyl group of the aglycone, usually located at position 3 or 7, whereas the C-glycosides have sugar groups bound to a carbon of the aglycone, usually 6-C or 8-C. The most common carbohydrates are rhamnose, glucose, galactose and arabinose. This chapter mainly introduces the methods of isolation and structure identification of flavonoids.

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“…2). The butanol fraction (2.9 g), which increased Ngn2 promoter activity by 1.5-fold, was subjected to ODS column chromatography followed by HPLC and yielded baicalin (6) [19] and isoverbascoside (7) [20]. The identities of these compounds were determined by comparing their spectral values with values from the literature.…”

Section: Resultsmentioning

confidence: 99%

Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter

et al. 2015

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A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells.

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Separation of Flavonoids from the Leaves of Oroxylum indicum by HSCCC

Cited by 51 publications

References 17 publications

Antimicrobial flavonoids from the twigs ofPopulus nigra × Populus deltoides

Antimicrobial flavonoids from the twigs ofPopulus nigra × Populus deltoides

Isolation and Structure Identification of Flavonoids

Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter

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