Separation of fatty acids, phospholipids and chloroplast pigments on Sephadex LH-20

Downey, W. K.; Murphy, Richard F.; Keogh, Myra

doi:10.1016/s0021-9673(00)83975-8

Cited by 17 publications

(4 citation statements)

References 23 publications

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“…Assays carried out by column chromatography, both with Shepadex LH-20 as indicated by Downey et al (1970), Yoshiura (1979), andShimizu (1971) and with Lobar silica gel (Jong and Woodlief, 1978), did not give the expected results, since although the fatty matter is eliminated, the retention times are excessively long, causing pigment alteration.…”

Section: Methodsmentioning

confidence: 91%

Chlorophyll and carotenoid presence in olive fruit (Olea europaea)

Mínguez‐Mosquera¹,

Garrido‐Fernández²

1989

J. Agric. Food Chem.

110

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Section: Methodsmentioning

confidence: 91%

Chlorophyll and carotenoid presence in olive fruit (Olea europaea)

Mínguez‐Mosquera¹,

Garrido‐Fernández²

1989

J. Agric. Food Chem.

110

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“…Sephadex LH-20 has been used for exclusion A commonly used method to purify plant extracts is chromatography of glycerides, fatty acids and phospholiquid chromatography in preparative scale. Two col-lipids (Joustra 1966, Downey et at. 1970, for adsorpumn materials that have found wide application in tion chromatography of polycyclic aromatic hydrocarorganic-chemical analyses are insoluble poly-iV-vinyl-bons (Wilk ef a/.…”

Section: Introductionmentioning

confidence: 99%

Chromatography of acid phytohormones on columns of Sephadex LH‐20 and insoluble poly‐N‐vinylpyrrolidone, and application to the analysis of conifer extracts

Sandberg

Dunberg

Odén

1981

Physiologia Plantarum

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The chromatographic behaviour of abscisic acid (ABA), indole‐3‐acetic acid (IAA), phenylacetic acid (PAA), and gibberellins A1, A4, A8, A9, A13 and A20 on columns of Sephadex LH‐20 and insoluble poly‐N‐vinylpyrrolidone (PVP) eluted with buffers of different pH values is described. PVP shows considerable batch differences that must be carefully checked. Chromatography of acidic ethyl acetate‐soluble fractions of Scots pine (Pinus sylvestris L.) extracts at pH 4.5 resulted in great losses of phytohormones, due to poor solubility of the extracts. If the extracts were applied to the column dissolved in buffer of pH 7.5, subsequent elution at pH 4.5 was possible with only small losses. The performance of the chromatographic column was strongly affected by the application volume. A combined PVP/Sephadex LH‐20 column eluted at pH 4.5 allows remarkable purification of pine and spruce (Picea abies (L.) Karst.) extracts, collection of IAA in a fraction that can be directly analyzed by e.g. the indolo‐α‐pyrone method, and collection of another fraction containing ABA, PAA and probably most of the known C19‐gibberellins; whereas the C20‐gibberellin A13 is eluted later (with IAA).

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“…This procedure involves the separation of the methyl esters of the components of a heat abused oil on a column of a hydroxylpropylated dextran gel (Sephadex LH-20) with chloroform. This is a procedure developed by Downey, et al, (63) as utilized by Ferren and Seery (64). Some prefer to call this technique "gel permeation chro-matography" and admittedly have good reason on the basis of the chemical properties involved, but we have utilized the designation "gel filtration chromatography" used by the gel manufacturer and of investigators who have used Sephadex gels in the past (63,64).…”

Section: Introductionmentioning

confidence: 99%

“…This is a procedure developed by Downey, et al, (63) as utilized by Ferren and Seery (64). Some prefer to call this technique "gel permeation chro-matography" and admittedly have good reason on the basis of the chemical properties involved, but we have utilized the designation "gel filtration chromatography" used by the gel manufacturer and of investigators who have used Sephadex gels in the past (63,64). Bly (65) extensively discussed the pros and cons of the terminology and suggested "size exclusion chromatography" as better defining the mechanism which he and Determann (66) have explained as the passing of molecules through the gel bed in the order of their decreasing size.…”

Section: Introductionmentioning

confidence: 99%

Chemical analysis of polymerization products in abused fats and oils

Waltking¹,

Seery²,

Bleffert³

1975

J Americ Oil Chem Soc

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For more than a decade, numerous analytical methods have been proposed for the detection and measurement of polymers in vegetable fats and oils. Many of the methods have been little more than laboratory curiosities, either because they were concemed with only very specific compounds or were too cumbersome and time consuming to become very popular. More recently, a number of methods in common use for analysis of fats and oils has been shown to be useful for indirectly measuring polymeric materials in heat abused oils. The present report shows, by the use of gel filtration chromatography, the validity of two of the indirect methods of estimating polymeric products of abused fats and oils. These methods are: the estimation of polymers through changes in the iodine value and the measurement of retention materials on a gas liquid chromatographic column. A new simplified internal standard gas liquid chromatographic procedure utilizing triglyceride standards also is presented. This latter method permits estimating the degree of degradation of vegetable fats and oils by any laboratory capable of determining the fatty acid composition of a sample by gas liquid chromatography.

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Separation of fatty acids, phospholipids and chloroplast pigments on Sephadex LH-20

Cited by 17 publications

References 23 publications

Chlorophyll and carotenoid presence in olive fruit (Olea europaea)

Chlorophyll and carotenoid presence in olive fruit (Olea europaea)

Chromatography of acid phytohormones on columns of Sephadex LH‐20 and insoluble poly‐N‐vinylpyrrolidone, and application to the analysis of conifer extracts

Chemical analysis of polymerization products in abused fats and oils

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