Separation of extracts from biological tissues into polycyclic aromatic hydrocarbon, polychlorinated biphenyl and polychlorinated dibenzo-p-dioxin/polychlorinated dibenzofuran fractions prior to analysis

O’Keefe, Patrick; Miller, John P.; Smith, Robert M.; Connor, Steven; Clayton, William; Storm, Robin

doi:10.1016/s0021-9673(97)00177-5

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Similar retention mechanisms can be attributed to porous graphitic carbon (PGC) phases when used under normal-phase conditions. 38 Other types of phases are based on modified silica gel carrying ligands exposing π-electron acceptor moieties. πelectron rich molecules like PAHs can form donor−acceptor complexes with these ligands.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Universal Route to Polycyclic Aromatic Hydrocarbon Analysis in Foodstuff: Two-Dimensional Heart-Cut Liquid Chromatography–Gas Chromatography–Mass Spectrometry

et al. 2015

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Analysis of polycyclic aromatic hydrocarbons (PAHs) in complex foodstuff is associated with complicated and work-intensive sample preparation. Chromatographic interference has to be faced in many situations. The scope of the current work was the development of a highly efficient two-dimensional heart-cut LC-LC-GC-MS method. Detection was performed with a time-of-flight mass spectrometer (TOF-MS) to allow for a comprehensive evaluation of the obtained data in terms of cleanup efficiency. Additionally, routine detection was performed with single quadrupole MS. An easy and quick generic sample preparation protocol was realized as a first step. During method development, focus was given to optimizing HPLC cleanup for complex foodstuff. Silica-, polymeric-, and carbon-based HPLC phases were tested. Coupling of silica gel to π-electron acceptor modified silica gel showed the best cleanup properties. A four rotary valve configuration allowed the usage of a single binary HPLC pump. Screening of several fatty and nonfatty food matrices showed the absence of unwanted matrix compounds in the cleaned-up PAH fraction down to the low picogram range using TOF-MS. Limits of quantitation (LOQ) were below 0.1 μg/kg for all EU priority PAHs. Recovery rates ranged from 82 to 111%. Validation data fully complied with EU Regulation 836/2011. Sample preparation was possible in 20 min. Interlacing of HPLC and GC allowed an average method runtime of 40 min per sample.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…Afterward, PAHs are eluted with an appropriate solvent, e.g., toluene, tetrahydrofuran (THF), or dichloromethane. Similar retention mechanisms can be attributed to porous graphitic carbon (PGC) phases when used under normal-phase conditions …”

Section: Resultsmentioning

confidence: 99%

Universal Route to Polycyclic Aromatic Hydrocarbon Analysis in Foodstuff: Two-Dimensional Heart-Cut Liquid Chromatography–Gas Chromatography–Mass Spectrometry

et al. 2015

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“…Another potential sorbent is Pyrenylbased silica (PYE) [18]. But its loading capacity is approximately half of PGC (3 $ 5mg/g for planar non-ortho PCBs [19]), and can only be operated between 0 C and 25 C [20]. The main drawback hindering the wide use of PGC column in PCDD/Fs analysis is the production of broad peaks when backflushing is used to recover PCDD/Fs using solvents at room temperature, resulting in carry-over and cross-contamination problems [21].…”

Section: Introductionmentioning

confidence: 99%

Method development of combining pressurized liquid extraction and off-line HPLC fractionation with a porous graphite carbon column for the analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in environmental samples

Chen

et al. 2013

International Journal of Environmental Analytical Chemistry

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Organic pollutants in wild ducks from New York state: I. Interspecies differences in concentrations and congener profiles of PCBs and PCDDs/PCDFs

O’Keefe

Clayton

Connor

et al. 2006

Science of The Total Environment

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Separation of extracts from biological tissues into polycyclic aromatic hydrocarbon, polychlorinated biphenyl and polychlorinated dibenzo-p-dioxin/polychlorinated dibenzofuran fractions prior to analysis

Cited by 4 publications

References 9 publications

Universal Route to Polycyclic Aromatic Hydrocarbon Analysis in Foodstuff: Two-Dimensional Heart-Cut Liquid Chromatography–Gas Chromatography–Mass Spectrometry

Universal Route to Polycyclic Aromatic Hydrocarbon Analysis in Foodstuff: Two-Dimensional Heart-Cut Liquid Chromatography–Gas Chromatography–Mass Spectrometry

Method development of combining pressurized liquid extraction and off-line HPLC fractionation with a porous graphite carbon column for the analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in environmental samples

Organic pollutants in wild ducks from New York state: I. Interspecies differences in concentrations and congener profiles of PCBs and PCDDs/PCDFs

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