Separation of excitation and detection coils for in vivo detection of superparamagnetic iron oxide nanoparticles

Loosdrecht, Melissa Mathilde van de; Waanders, Sebastiaan; Krooshoop, Hendrikus J.G.; Haken, Bernard ten

doi:10.1016/j.jmmm.2018.12.012

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…Magnetic iron oxide nanoparticles (NPs), such as magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ), have been found and applied in a wide range of biomedical applications [1,2,3,4,5,6,7,8,9], including magnetic resonance imaging, magnetic hyperthermia, cancer therapy, and drug delivery; they also find applications in catalysis [10,11,12] and in magnetic separation [13,14]. Among other metal nanoparticles, iron oxide nanoparticles have been more extensively studied in clinical practice [15].…”

Section: Introductionmentioning

confidence: 99%

Fe3O4 Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy

et al. 2019

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Magnetic Fe3O4 nanoparticles (NPs) and their surface modification with therapeutic substances are of great interest, especially drug delivery for cancer therapy, including boron-neutron capture therapy (BNCT). In this paper, we present the results of boron-rich compound (carborane borate) attachment to previously aminated by (3-aminopropyl)-trimethoxysilane (APTMS) iron oxide NPs. Fourier transform infrared spectroscopy with Attenuated total reflectance accessory (ATR-FTIR) and energy-dispersive X-ray analysis confirmed the change of the element content of NPs after modification and formation of new bonds between Fe3O4 NPs and the attached molecules. Transmission (TEM) and scanning electron microscopy (SEM) showed Fe3O4 NPs’ average size of 18.9 nm. Phase parameters were studied by powder X-ray diffraction (XRD), and the magnetic behavior of Fe3O4 NPs was elucidated by Mössbauer spectroscopy. The colloidal and chemical stability of NPs was studied using simulated body fluid (phosphate buffer—PBS). Modified NPs have shown excellent stability in PBS (pH = 7.4), characterized by XRD, Mössbauer spectroscopy, and dynamic light scattering (DLS). Biocompatibility was evaluated in-vitro using cultured mouse embryonic fibroblasts (MEFs). The results show us an increasing of IC50 from 0.110 mg/mL for Fe3O4 NPs to 0.405 mg/mL for Fe3O4-Carborane NPs. The obtained data confirm the biocompatibility and stability of synthesized NPs and the potential to use them in BNCT.

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Section: Introductionmentioning

confidence: 99%

Fe3O4 Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy

et al. 2019

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“…If laparotomy is performed, such a handheld probe may be used intraoperatively along with fluorescence-imaging to help guide SLN localization. For laparoscopic or robotic-assisted procedures, ‘drop-in’ magnetometer probes capable of fitting through trocar ports are currently being developed [ 32 , 33 ] and would provide a less invasive means for intraoperative magnetometer detection. Such probes are not currently commercially available, and there exist several non-trivial technical hurdles that have to be overcome—for example, reducing the probe size to fit through a trocar port greatly reduces magnetometer sensitivity, and metal laparoscopic or robotic instruments can induce spurious magnetometer signals.…”

Section: Discussionmentioning

confidence: 99%

Preclinical evaluation of sentinel node localization in the stomach via mannose-labelled magnetic nanoparticles and indocyanine green

Cousins

Krishnan

et al. 2023

Surg Endosc

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Background Gastrectomy with extended (D2) lymphadenectomy is considered standard of care for gastric cancer to provide the best possible outcomes and pathologic staging. However, D2 gastrectomy is a technically demanding operation and reported to be associated with increased complications and mortality. Application of sentinel lymph node (SLN) concept in gastric cancer has the potential to reduce patient morbidity; however, SLN techniques are not established for gastrectomy, in part due to lack of practical tracers. An effective and convenient tracer with enhanced SLN accumulation is critically needed. Methods Mannose-labelled magnetic tracer ‘FerroTrace’ and fluorescent dye indocyanine green (ICG) were injected laparoscopically into the stomach submucosa of 8 healthy swine under general anaesthesia. Intraoperative fluorescence imaging was used to highlight draining lymphatic pathways containing ICG, while preoperative T2-weighted MRI and ex vivo magnetometer probe measurements were used to identify nodes containing FerroTrace. Lymphadenectomy was performed either robotically (n = 2) or via laparotomy (n = 6). Results Mixing ICG and FerroTrace ensured concurrence of fluorescent and magnetic signals in SLNs. An initial trial with robotic dissection removed all magnetic LNs (n = 4). In the subsequent laparotomy study that targeted all ICG-LNs based on intraoperative fluorescence imaging, dissection removed an average of 4.7 ± 1.2 fluorescent, and 2.0 ± 1.3 magnetic LNs per animal. Both MRI and magnetometer detected 100% of SLNs (n = 7). FerroTrace demonstrated high specificity to SLNs, which contained 76 ± 30% of total lymphotropic iron, and 88 ± 20 % of the overall magnetometer signal. Conclusions Through utilisation of this dual tracer approach, SLNs were identified via preoperative MRI, visualised intraoperatively with fluorescence imaging, and confirmed with a magnetometer. This combination pairs the sensitivity of ICG with SLN-specific FerroTrace and can be used for reliable SLN detection in gastric cancer, with potential applications in neoadjuvant therapy. Graphical abstract

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“…As such, in settings where pseudo‐dynamic MRI is not possible or where the first MRI scan shows multiple draining LNs, we propose that magnetometer signal stratification between node levels could be used to identify the SLN and potentially provide a signal threshold level—such as the “10%” criterion opted to help define magnetic SLNs in breast cancer 32 . In addition, future development of specialized “drop‐in” probes could aid intraoperative signal confirmation during robotic or laparoscopic SLNB, as has been demonstrated with gamma‐guidance in the abdomen and pelvis 33 and is currently being researched for magnetic‐guidance using novel magnetometer probe designs 34,35 …”

Section: Discussionmentioning

confidence: 99%

“… 32 In addition, future development of specialized “drop‐in” probes could aid intraoperative signal confirmation during robotic or laparoscopic SLNB, as has been demonstrated with gamma‐guidance in the abdomen and pelvis 33 and is currently being researched for magnetic‐guidance using novel magnetometer probe designs. 34 , 35 …”

Section: Discussionmentioning

confidence: 99%

Preclinical feasibility of robot‐assisted sentinel lymph node biopsy using multi‐modality magnetic and fluorescence guidance in the head and neck

et al. 2022

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Background: Sentinel lymph node biopsy (SLNB) is a staging procedure dependent on accurate mapping of draining lymphatics via tracers. Robotassisted SLNB enables access to multiple neck levels with a single incision and intraoperative fluorescence guidance to the SLN.Methods: Lymphatic mapping in swine was done using a magnetic tracer and fluorescent dye, injected into the tongue. MRI preoperatively mapped lymphatic spread of the magnetic tracer. Dissection was performed using a da Vinci Xi robot guided by fluorescence-imaging of the dye. Results: Robot-assisted SLNB was successfully performed in all animals (n = 5). A novel MRI protocol differentiated SLNs (n = 6) from lower echelon nodes (n = 11) based on flow progression. Fluorescence imaging provided valuable intraoperative guidance and correlated with magnetic-positive nodes.Conclusions: This study demonstrates preclinical feasibility of a robot-assisted approach to SLNB using magnetic and fluorescent tracers in the head and neck, enabling both preoperative mapping and intraoperative guidance.

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Separation of excitation and detection coils for in vivo detection of superparamagnetic iron oxide nanoparticles

Cited by 11 publications

References 23 publications

Fe3O4 Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy

Fe3O4 Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy

Preclinical evaluation of sentinel node localization in the stomach via mannose-labelled magnetic nanoparticles and indocyanine green

Preclinical feasibility of robot‐assisted sentinel lymph node biopsy using multi‐modality magnetic and fluorescence guidance in the head and neck

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