Separation of ethoxylated alcohol sulfates by capillary electrophoresis using indirect UV detection

Goebel, Lisa K.; McNair, Harold M.; Rasmussen, Henrik T.; McPherson, Bruce P.

doi:10.1002/mcs.1220050107

Cited by 12 publications

(1 citation statement)

References 8 publications

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“…A second approach is to add a co-ion as a buffer. This method has been used with addition of phosphate [45], carbonate [46], acetate [47], 2-(cyclohexylamino)ethanesulfonic acid [48], and borate [42,49,53] to BGEs for detection of anions. Whilst this may provide adequate buffering capacity, the introduction of a co-ion and the resultant competitive displacement by analyte ions can lead to a loss of detection sensitivity and the occurrence of system peaks which may interfere with detection of analytes.…”

Section: Buffering Of Electrolytes For Indirect Photometric Detectionmentioning

confidence: 99%

Enhancement of detection sensitivity for indirect photometric detection of anions and cations in capillary electrophoresis

2003

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This review focuses on the indirect photometric detection of anions and cations by capillary electrophoresis. Special emphasis has been placed on the sensitivity of the technique and approaches taken to enhance detection limits. Theoretical considerations and requirements have been discussed, including buffering, detection sensitivity, separation of cations, and detector linearity. A series of tables detailing highly absorbing probes and the conditions of their use for indirect photometric detection are included.

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Section: Buffering Of Electrolytes For Indirect Photometric Detectionmentioning

confidence: 99%

Enhancement of detection sensitivity for indirect photometric detection of anions and cations in capillary electrophoresis

2003

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Determination of surfactants by capillary electrophoresis

Heinig

Vogt

1999

Electrophoresis

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Indirect Detection Methods in Capillary Electrophoresis

Shamsi

2000

Encyclopedia of Analytical Chemistry

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An overview is given of existing indirect detection methods of inorganic and organic anions in capillary zone electrophoresis (CZE). Kohlrausch's regulating function (KRF), used by several researchers to predict the detection sensitivity of ultraviolet (UV)‐inactive analytes in CZE, is reviewed. Sensitive indirect photometric detection (IPD) of ionic compounds in CZE is largely governed by the value of the change in absorbance in a solute zone. This in turn depends on a transfer ratio (TR), ratio of the charge, and ratio of the electrophoretic mobility of the analyte and IPD reagent. The influence of other experimental parameters, such as pH, type and concentration of the absorbing electrolyte, detection wavelength, and absorption pathlength, on detection sensitivity are also critically discussed. An extensive part of this review deals with the separation of various classes ofionic analytes using capillary zone electrophoresis/indirect photometric detection (CZE/IPD). Different approaches for simultaneous separation and indirect detection of nonabsorbing anions and cations are evaluated. From this evaluation, some rules of thumb as to when these approaches will be most effective are drawn. In addition, indirect fluorescence detection (IFD) in CZE and micellar electrokinetic chromatography (MEKC) are reviewed and possible application areas are highlighted. Some recent developments in other indirect detection modes, such as indirect chemiluminescence detection (ICLD) and indirect conductivity detection (ICD), are outlined.

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Separation of ethoxylated alcohol sulfates by capillary electrophoresis using indirect UV detection

Cited by 12 publications

References 8 publications

Enhancement of detection sensitivity for indirect photometric detection of anions and cations in capillary electrophoresis

Enhancement of detection sensitivity for indirect photometric detection of anions and cations in capillary electrophoresis

Determination of surfactants by capillary electrophoresis

Indirect Detection Methods in Capillary Electrophoresis

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