Separation of DNA restriction fragments by capillary electrophoresis using coated fused silica capillaries

Strege, Mark A.; Lagu, Avinash L.

doi:10.1021/ac00013a010

Cited by 160 publications

(55 citation statements)

References 24 publications

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“…Primers were designed in exonic regions flanking likely intron sites (supplemental Table 1 at http:/ /www.genetics.org/supplemental/) according to Palumbi and Baker (1994). To increase our ability to detect small length differences, we used capillary electrophoresis, which can discriminate PCR products differing by as little as a single base pair (Swerdlow and Gesteland 1990;Strege and Lagu 1991).…”

Section: Methodsmentioning

confidence: 99%

Evolutionary Strata on the X Chromosomes of the Dioecious Plant Silene latifolia: Evidence From New Sex-Linked Genes

et al. 2007

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Despite its recent evolutionary origin, the sex chromosome system of the plant Silene latifolia shows signs of progressive suppression of recombination having created evolutionary strata of different X-Y divergence on sex chromosomes. However, even after 8 years of effort, this result is based on analyses of five sex-linked gene sequences, and the maximum divergence (and thus the age of this plant's sex chromosome system) has remained uncertain. More genes are therefore needed. Here, by segregation analysis of intron size variants (ISVS) and single nucleotide polymorphisms (SNPs), we identify three new Y-linked genes, one being duplicated on the Y chromosome, and test for evolutionary strata. All the new genes have homologs on the X and Y chromosomes. Synonymous divergence estimated between the X and Y homolog pairs is within the range of those already reported. Genetic mapping of the new X-linked loci shows that the map is the same in all three families that have been studied so far and that X-Y divergence increases with genetic distance from the pseudoautosomal region. We can now conclude that the divergence value is saturated, confirming the cessation of X-Y recombination in the evolution of the sex chromosomes at 10-20 MYA.

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Section: Methodsmentioning

confidence: 99%

Evolutionary Strata on the X Chromosomes of the Dioecious Plant Silene latifolia: Evidence From New Sex-Linked Genes

et al. 2007

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“…For example, Strege and Lagu [5] describe the separation in 18 min of a 1 kbp DNA ladder (a size standard useful for the restriction mapping of plasmids) in a 0.5% methyl cellulose solution, while Chiari et ul. [37] chose a concentration of 4.5% linear PAA to provide the same separation, with similar results but a much longer analysis time (120 min).…”

Section: The Importance Of Polymer Type: Comparison Of Dna Separationmentioning

confidence: 99%

The effects of polymer properties on DNA separations by capillary electrophoresis in uncross‐linked polymer solutions

1996

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Low-viscosity, aqueous solutions of hydrophilic linear polymers have been shown to be useful for the separation of DNA restriction fragments by capillary electrophoresis (CE). However, the choice of polymer type, size, and concentration remains largely empirical, because the mechanism of high-field electrophoretic DNA separations in polymer solutions is not well understood. To assist in elucidating the mechanism of DNA separation, we experimentally investigated the effects of polymer properties such as stiffness (persistence length), average molecular mass, polydispersity, and hydrophilicity on the separation of DNA ranging from 72 bp to 23 kbp. This was accomplished by comparing the results of DNA separations obtained by counter-migration CE in dilute and semidilute solutions of linear polyacrylamide (PAA), hydroxyethyl-cellulose (HEC), and hydroxypropylcellulose (HPC) polymers of several different average molecular masses.

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“…Micro-tensile testers are designed for specific kind of materials. To date, despite the importance of silica glass in various technological fields such as optics, electronics and chemistry [23][24][25][26], such a tool is missing for investigating the micromechanical behavior of these materials.…”

Section: Introductionmentioning

confidence: 99%

A Monolithic Micro-Tensile Tester for Investigating Silicon Dioxide Polymorph Micromechanics, Fabricated and Operated Using a Femtosecond Laser

Athanasiou

Bellouard

2015

Micromachines

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Mechanical testing of materials at the microscales is challenging. It requires delicate procedures not only for producing and handling the specimen to be tested, but also for applying an accurate and controlled force. This endeavor is even more challenging when it comes to investigating the behavior of brittle materials such as glass. Here, we present a microtensile tester for investigating silica glass polymorphs. The instrument is entirely made of silica and for which the same femtosecond laser is not only used for fabricating the device, but also for operating it (loading the specimen) as well as for performing in situ measurements. As a proof-of-concept, we present a stress-strain curve of fused silica for unprecedented high tensile stress of 2.4 GPa, as well as preliminary results of the elastic modulus of femtosecond laser-affected zones of fused silica, providing new insights on their microstructures and mechanical behavior.

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Separation of DNA restriction fragments by capillary electrophoresis using coated fused silica capillaries

Cited by 160 publications

References 24 publications

Evolutionary Strata on the X Chromosomes of the Dioecious Plant Silene latifolia: Evidence From New Sex-Linked Genes

Evolutionary Strata on the X Chromosomes of the Dioecious Plant Silene latifolia: Evidence From New Sex-Linked Genes

The effects of polymer properties on DNA separations by capillary electrophoresis in uncross‐linked polymer solutions

A Monolithic Micro-Tensile Tester for Investigating Silicon Dioxide Polymorph Micromechanics, Fabricated and Operated Using a Femtosecond Laser

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