Separation of Di-dansyl amino acids by MEKC with a co-surfactant

Skočir, E.; Prošek, Mirko; Oskomič, M.

doi:10.1007/bf02466393

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…The employment of a cosurfactant not only affects the polarity of the mobile phase, but also changes the EOF and the partition coefficient of the solutes in the micelles. This effect was previously studied on four didansyl derivatives by Skocir et al (1997a). Our investigation was focused on the influence of isobutanol on the behavior of nine essential amino acids.…”

Section: Resultsmentioning

confidence: 98%

“…Furthermore, organic modifiers can be used in MECC to improve the separation, extending the migration-time window, improving the resolution, and modifying the migration order of analytes due to their effect on electrokinetic migrations and the capacity factor of homologous compounds (Liu et al, 1998). Some applications in food chemistry have involved the use of MECC for the quantitative analysis of dansyl derivatives of amino acids in samples from different sources, including corn seed flour (Skocir and Prosek, 1995), fish (Skocir et al, 1997a), maize seed and seaweed (Skocir et al, 1997b), and honey (Corradini et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Rapid Analysis of Essential and Branched-Chain Amino Acids in Nutraceutical Products by Micellar Electrokinetic Capillary Chromatography

Cavazza¹,

Corradini²,

Lauria³

et al. 2000

J. Agric. Food Chem.

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A rapid method for the analysis of dansylated essential and branched-chain amino acids (BCAAs) by micellar electrokinetic capillary chromatography (MECC) is reported. Optimization of analytical conditions has been carried out, evaluating the influence on the performance of several parameters such as sodium dodecyl sulfate (SDS) concentration in the running electrolyte, temperature, and voltage. The effect of the addition of small amounts of isobutanol to the electrolyte has also been investigated. The best separation in the shortest time with a 37 cm capillary was obtained employing a 20 mM Borax buffer (pH 9.1) + 70 mM SDS at 25 degrees C and 20 kV. Under these conditions a mixture of nine essential amino acids was analyzed in 7 min, while separation of BCAAs occurred in less than 4 min. Using a shorter capillary (20 cm to the detector), the BCAA separation was performed in only 2.5 min. The method was applied to the quantitative analysis of amino acids in three commercial nutraceutical preparations. Assessment of analytical performance in terms of precision, linearity, and limit of detection has also been reported.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Rapid Analysis of Essential and Branched-Chain Amino Acids in Nutraceutical Products by Micellar Electrokinetic Capillary Chromatography

Cavazza¹,

Corradini²,

Lauria³

et al. 2000

J. Agric. Food Chem.

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“…Of the numerous possibilities, separations of dinitrophenyl (DNP)-, dansyl-, and fluorescein isothiocyanate (FITC)-derivatives have been widely explored ( [18][19][20], for review see [15]). However, detection by UV absorbance in channels manufactured from other materials than fused silica can be difficult even if the derivatized amino acids bear a distinct chromophore.…”

Section: Introductionmentioning

confidence: 99%

Plastic substrates based separation channels in electromigration techniques

Charvátová

Deyl

Klevar

et al. 2004

Journal of Chromatography B

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Quantitative determination of some amino acids in maize seed and seaweed by micellar electrokinetic chromatography

et al. 1997

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This article presents the utilization of micellar electrokinetic chromatography for the quantitative determination of some amino acids as dansyl and didansyl derivatives of maize seed and spirulina‐type seaweed. During the work, the repeatability of hydrolysis, derivatization, and analysis were evaluated. Some selected free amino acids were derivatized at different concentrations and calibration curves were prepared. The absolute content of individual amino acids was determined in a few types of maize seed flour and in one type of seaweed. To cover the entire electropherogram, amino acids from the beginning, middle, and end of the electropherogram were selected. A newly developed method was used for the determination and quantitation of the di‐dansyl derivative of lysine. © 1997 John Wiley & Sons, Inc. J Micro Sep 9: 451–457, 1997

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Separation of Di-dansyl amino acids by MEKC with a co-surfactant

Cited by 8 publications

References 23 publications

Rapid Analysis of Essential and Branched-Chain Amino Acids in Nutraceutical Products by Micellar Electrokinetic Capillary Chromatography

Rapid Analysis of Essential and Branched-Chain Amino Acids in Nutraceutical Products by Micellar Electrokinetic Capillary Chromatography

Plastic substrates based separation channels in electromigration techniques

Quantitative determination of some amino acids in maize seed and seaweed by micellar electrokinetic chromatography

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