Separation of Chromophoric Substance from Amur Cork Tree Using GC-MS

Abstract

Amur cork tree was extracted in methanol with the purpose of investigating the most effective extraction procedure for detecting the chromophore using the GC-MS analysis. Different procedures of waterbath and hotplate extractions were carried out and five different GC-MS instrument parameters including the operating temperatures in the GC capillary column and the MSD scan range were tested for their efficiencies. Berberine was determined by the detection of dihydroberberine at 15.0 min r.t. Hotplate was a better device for extracting amur cork tree than waterbath shaker either with or without presoaking in the room temperature. Water was not an adequate extraction medium for the berberine detection. The most effective GC-MS parameter was Method 4; the initial temperature at 50 o C followed by the temperature increase of 23 o C/min until 210 o C, then increase of 30 o C/min until the final temperature reach at 305 o C, then hold for 14 minutes to maintain the total run time 24.12 minutes. The MSD scan range for Method 4 was 35~400m/z.

Examination of Berberine Dye using GC-MS after Selective Degradation Treatments

“…Instead, the control sample of berberine dye showed a prominent peak at 15.03 min, followed by 16,35 min and 13.45 min in the repeated analyses, and the products were successfully assigned as dihydroberberine, 2-pteridinamine, 6,7-dimethyl-N-[(trimethylsilyl)oxy]-, and 8-methoxy-11-[3-methylbutyl]-11H-indolo[3,2-c]-quinoline, 5-oxide respectively by the analysis of NIST library database. This phenomenon coincided with the result of the previous investigation on the GC-MS analysis of dye extracted from the amur cork tree (Ahn, 2009). And as explained in Ahn (2009) with the theoretical base on Turner et al (2008aTurner et al ( , 2008b, Song et al (2002), and Choi (2005), it can be said that dihydroberberine is a fingerprint product for identifying the presence of berberine dye.…”

“…This phenomenon coincided with the result of the previous investigation on the GC-MS analysis of dye extracted from the amur cork tree (Ahn, 2009). And as explained in Ahn (2009) with the theoretical base on Turner et al (2008aTurner et al ( , 2008b, Song et al (2002), and Choi (2005), it can be said that dihydroberberine is a fingerprint product for identifying the presence of berberine dye. And to a lesser degree, 2-pteridinamine, 6,7-dimethyl-N-[(trimethylsilyl)oxy]-and 8-methoxy-11-[3-methylbutyl]-11H-indolo[3,2-c]-quinoline, 5-oxide may also act as a supplementary fingerprint for the trace of berberine dye.…”

“…Products were separated on a Hewlett Packard 19091s-433 capillary column (HP-5MS, 30cm×250i.d., 0.25µm nominal film thickness). The instrument parameter for running the GC-MS was selected based upon the experimental result obtained from the previous study on the separation of berberine dye from the amur cork tree (Ahn, 2009 C and the mass spectra were recorded at scan range of 75~400m/z. The assignment of possible degradation products was based on the match with standard mass spectrum available in the GC-MS library database.…”