Separation of cephalosporins on thin silica gel layers impregnated with transition metal ions and by reversed‐phase TLC

Abstract

Cephalosporin antibiotics were separated on thin layer plates impregnated with transition metal ions, Mn(2+), Fe(2+), Co(2+), Ni(2+) and Cu(2+), using different concentrations. Various solvent systems were developed for the study and were used for separation of these analytes. Impregnation was observed to have an effect on hR(F) values, removed tailing of analytes and improved the resolution. The results have been discussed for each metal ion and compared, and the best conditions of separation have been identified. Activation time of thin layer plates impregnated with 0.1% FeSO(4) was found to affect both hR(F) values and resolution of cephalosporins. New solvent systems are reported for both normal phase and reversed-phase TLC.

Basic TLC Techniques, Materials, and Apparatus

“…Chiral selectors have been impregnated into silica gel layers for NP-TLC enantiomer separations, e.g., the macrocyclic antibiotic vancomycin for DL-amino acids (199) and L-lysine and L-arginine for /3-adrenergic blocking agents (120). Unmodified cellulose has been used for separation of enantiomeric amino acids and peptides and other compounds (e.g., 121).…”

Chromatographic Separation Studies of Penicillins, Cephalosporins and Carbapenems on Transition-Metal Silicate Modified Silica Layers

“…2 The chromatographic behavior of penicillins has been studied on thin layers of titanic silicate 3 and stannic arsenate-cellulose. 4 Cephalosporins have been separated on 4% methanolic CTAB impregnated silica layers, 5 double hydroxide layers of Al(III) and Mg(II), 6 an EDTA impregnated silica gel G plate, 7 a stannic oxide layer, 8 silica gel G impregnated with zinc ferrocyanide, 9 silica-gel G layers impregnated with transition-metal ions 10 and a densitometric thin-layer chromatography (TLC) method. 11 Various cephalosporins have been identified by color reactions with fluorescamine, 12 dragendroff's reagent.…”

Utilização e recuperação de sílica gel impregnada com nitrato de prata

“…1 Entretanto, o emprego de outros sais inorgânicos é conhecido e entre estes se destacam os sais de cobre, tálio e níquel. [2][3][4] O nitrato de prata associado à sílica-gel tem uma longa história de uso como suporte cromatográfico. Elberz, 5 em 1937, e Lucas e Winstein, 6 em 1938, foram os primeiros a reportar que íons prata poderiam se complexar com alquenos.…”