Separation of centrifuged whole blood and pooled buffy coats using the new CompoMat G5: 3 years experience

Abstract

The same high-quality blood components can be prepared by using the CompoMat G5 as previously with other semi-automated devices. Improvement was realized by automation of the opening process by the use of collection systems with CF closures, which led to a decrease in discarded units and workload.

Detection of cocaine and its metabolites in whole blood and plasma following a single dose, controlled administration of intranasal cocaine

“…[1][2][3][4][5][6]11,16 Whole blood and plasma concentrations of COC, BZE, and EME When blood is centrifuged, it can be separated into two or three layers, with 55% consisting of plasma, 45% of erythrocytes, and 1% of leukocytes mixed with platelets (referred to as the buffy coat). 17 A drug that is equally distributed between plasma and erythrocytes will result in a blood to plasma drug concentration ratio of unity (1).…”

A new preparation method for red blood cells for intrauterine transfusion enabling reduction of donor exposure

“…Five‐hundred milliliter WB donations were collected and processed into LR RBCs as described earlier . LR RBCs for IUT were prepared from routine LR RBCs in SAGM 60 hours after collection to reflect current routine practice.…”

“…Five-hundred milliliter WB donations were collected and processed into LR RBCs as described earlier. 7 LR RBCs for IUT were prepared from routine LR RBCs in SAGM 60 hours after collection to reflect current routine practice. The LR RBC units were centrifuged for 30 minutes at 3835 3 g (maximum acceleration, slow stop 2, Sorvall RC12BP, Thermo Fisher Scientific, Asheville, NC) resulting in an accumulated centrifugal effect (ACE) of approximately 21 3 10 7 .…”

Processing and storage of blood components: strategies to improve patient safety

“…70 At present, with automated cannula breakers, "electronic eyes", clamps, presses, detectors, flow speed controllers, and sealing heads, the blood separation process is fully controlled. 71 It needs less human oversight and has led to more standardized and uniform composition of the blood components. A further benefit is that these devices allow full documentation of the processing by barcode scanning (staff ID, instrument ID, bag ID, program used, time, components obtained, OK/not OK) and integration into the main blood bank software.…”

“…Particularly, the composition of the buffy coat, as source for further platelet isolation, became highly standardized. 70,71 These automated separators still require a manual step where the centrifuged whole blood is transferred to the separator. Since the 1990s, machines that integrate centrifugation and component separation in one process have been developed.…”