Separation of Carboxylate Ions on the Paper Chromatogram

Brown, F.; Hall, L.P.

doi:10.1038/166066b0

Cited by 83 publications

(12 citation statements)

References 5 publications

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“…No movement of starting spots was obtained upon attempts to determine the C2-C 0 fatty acid content by the method of Brown and Hall (1950) which indicates the absence of short chain fatty acids in these samples.…”

Section: Resultsmentioning

confidence: 94%

“…Reference standards of highly purified fatty acids were run with all samples. Fractions were chromatographed for C2-C10 fatty acids by the method of Brown and Hall (1950), and for C12-C18 acids by the reversed phase method of Mangold et al (1955). The procedures of Partridge (1948) and Davies (1956) were employed for detection of sugars in phospholipid fractions.…”

Section: Experimental Methodsmentioning

confidence: 99%

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LIPIDS OF BACILLUS STEAROTHERMOPHILUS ,

Long

Williams

1960

J Bacteriol

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The relationship of cellular lipids to thermophily and the heat resistance of bacterial spores has been studied but with occasional contradictory results. Explanation for differences may lie in attempts to compare the lipids of the same or different species of microorganism grown under widely varying cultural conditions. Evidence that cultural conditions alone may influence lipid content has been offered by Larson and Larson (1922), Heide (1939), and Starkey (1946). Leathes and Raper (1925) were among the first to attempt correlation of growth temperature with degree of unsaturation of cellular lipids. Using lipids of plants and animals, they found that highly unsaturated lipids were produced at low environmental temperatures, whereas the most saturated lipids were obtained at high temperatures. The lipids extracted from species of Aspergillus and Rhizopus by Terroine et al. (1927) conformed to this principle but Prill et al. (1935) with Aspergillus Jischeri, and Singh and Walker (1956) using A. nidulans, found an inverse correlation. Gaughran (1947) observed that the lipids of Bacillus subtilis decreased in quantity and unsaturation as the incubation temperature was raised above the optimum whereas under similar conditions the lipids of an unidentified thermophile were constant. Early studies on the effect of lipids on heat resistance of bacterial spores have been reviewed by Gaughran (1947). Further evidence that lipids may be important in heat resistance was provided by Sugiyama (1951), who was able to increase the heat resistance of spores of Clostridium botulinum by adding long chain fatty I Supported in part by a research grant from Swift and Company, Chicago, Illinois. 2 This work was taken from a dissertation submitted in partial fulfillment of the requirements for the Ph.D. degree at the

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Section: Resultsmentioning

confidence: 94%

Section: Experimental Methodsmentioning

confidence: 99%

LIPIDS OF BACILLUS STEAROTHERMOPHILUS ,

Long

Williams

1960

J Bacteriol

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“…All of these isolates were allocated to the genus Propionibacterium because each formed acetic and propionic acids as well as carbon dioxide in the lactate medium. The organic acids were determined by paper chromatography (Brown and Hall, 1950;Lugg and Overell, 1948). Carbon dioxide was measured manometrically.…”

Section: Experimental Methodsmentioning

confidence: 99%

Species of Propionibacterium Associated with Zapatera Spoilage of Olives

Plastourgos¹,

Vaughn²

1957

Applied Microbiology

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“…The lower fatty acids were separated according to the method of Brown & Hall (1950) in a mixture of n-butanol + 1-5 N-NH,OH (1 + 1 by vol.) on Whatman no.…”

Section: Manometric Methodsmentioning

confidence: 99%

The Physiology of Riboflavin Production by Eremothecium ashbyi

Капралек

1962

Journal of General Microbiology

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SUMMARYDuring the submerged batch cultivation of a riboflavin-producing strain of Eremothecium ashbyi three phases were observed. The first phase was characterized by rapid growth of mycelium, rapid utilization and oxidation of glucose and a decrease of pH value caused by accumulation of pyruvic acid. Subsequently acetoin accumulated in the medium. Glucose was oxidized incompletely since only 1 -8 pmole oxygen were consumed/pmole glucose utilized. The end of this phase was marked by exhaustion of glucose and cessation of growth. The second phase began with sporulation and was characterized by rapid synthesis of cell-bound riboflavin. Simultaneously a rapid increase in catalase activity and decreases of pyruvate and acetoin were observed. This was accompanied by a marked decrease in Qo, on glucose while the Qo, on pyruvate, threonine or acetaldehyde increased to a maximum. Ammonia accumulated in the medium and alkaline pH values were reached. The third phase was characterized by autolysis of mycelium which led to the release of riboflavin and to a decrease of enzymic activities. A comparison of all important physiological parameters was made with two strains of E. ashbyi of different riboflavin productivity. On the basis of correlation between riboflavin formation, catalase activity and respiration on acetaldehyde, an hypothesis is proposed to explain overproduction of riboflavin by a shift from the initial cytochrome type of terminal respiration to the flavoprotein type which is, however, accompanied by over-production of the flavin prosthetic group.

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Separation of Carboxylate Ions on the Paper Chromatogram

Cited by 83 publications

References 5 publications

LIPIDS OF BACILLUS STEAROTHERMOPHILUS ,

LIPIDS OF BACILLUS STEAROTHERMOPHILUS ,

Species of Propionibacterium Associated with Zapatera Spoilage of Olives

The Physiology of Riboflavin Production by Eremothecium ashbyi

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