Separation of Bone Marrow Cells by Sedimentation at Unit Gravity

Peterson, Elbert A.; Evans, Warren H.

doi:10.1038/214824a0

Cited by 127 publications

(25 citation statements)

References 4 publications

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“…The first method, i.e., velocity sedimentation at unit gravity, gives separations which are probably based primarily on cell size (8,9), while those with the second method, Le., density gradient centrifugation, are based on density differences between the different cell types (5).…”

mentioning

confidence: 99%

Separation of Somatotrophs from the Rat Adenohypophysis by Velocity and Density Gradient Centrifugation

Hymer¹,

Kraicer²,

Bencosme³

et al. 1972

Experimental Biology and Medicine

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mentioning

confidence: 99%

Separation of Somatotrophs from the Rat Adenohypophysis by Velocity and Density Gradient Centrifugation

Hymer¹,

Kraicer²,

Bencosme³

et al. 1972

Experimental Biology and Medicine

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“…[2]. [4] and [6] and particle radius from culated for each of the erythrocyte species assuming do • 2X (12). The results, obtained in the absence of DMSO, are listed in Table 2, and are seen to vary between 4.4 x 10-15 and 6.6 x 10-15 ergs.…”

Section: Interaction Energiesmentioning

confidence: 99%

Enhanced Erythrocyte Suspension Layer Stability Achieved by Surface Tension Lowering Additives

Omenyi

Snyder

Absolom

et al. 1982

Journal of Dispersion Science and Technology

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An electrostatic repulsion -van der Waals attraction mechanism was used to explain the stability of erythrocyte suspensions layered on a 020 cushion. By lowering the surface tension of D20 to values around 65 ergs/cm 2 by admixture of varying concentrations of dimethyl sulfoxide (DMSO), the van der Waals attraction between cells was reduced to zero. The zeta potentials, as measured by microelectrophoresis, were reduced also by DMSO but at a slower rate than for the surface tensions 307 308 OMENYI ET AL. as shown by total interaction energy calculations. In the presence of low van der Waals attraction and at large negative surface potentials, high cell concentrations were supported on a°2 0 cushion without the suspension layer becoming unstable. Reduction in the electrostatic repulsion while maintaining low van der Waals attraction significantly reduced the suspension layer stability. Clearly defined secondary minima varying in depths from 3.9kT to 1.BkT at separation distances varying from ssK to 6sK were obtained from the energy curves for the cells in the absence of OMSO. Addition of OMSO of varying concentrations decreased the secondary minima to very low values or zero at DMSO concentrations between 11% and 14% (v/v). It was concluded that maximum stability of erythrocyte suspension layers could be attained in the absence of cross-linking ions, at high zeta potentials and low van der Waals attraction, and that the technique of the determination of the maximum stability with respect to droplet sedimentation could serve as another alternative method for the study of surface tensions of biological particles. The surface tension values obtained via the droplet sedimentation technique were confirmed independently by means of freezing front exclusion. The values obtained by the two methods agree well confirming the feasibility of using the droplet sedimentation method.

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“…The above bone marrow cell suspension was overlayed on the BSA solution and centrifuged for 15 min at 2,700 X g. Fractionated cells from centrifuges were stained with Giemsa stain and peroxidase stain to determine their morphologic characteristics (Peterson and Evans 1967).…”

Section: Methodsmentioning

confidence: 99%

The in vitro development of immunoglobulin producing cells from the human bone marrow null lymphocyte.

Kuroyanagi

Kura²

1981

Tohoku J. Exp. Med.

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The distribution of human bone marrow lymphocyte population was studied on twenty normal adults. The distribution of bone marrow lymphocytes were as follows : null lymphocytes 79.4%, E-RFC 8.9%, Thy+ lymphocytes 0.4%, Ty 0.9%, Tµ 1.4%, SmIg+ cells 7.8%, EAC-RFC 11.7%, EA-RFC 6.2%, and K cells 2.7%. Bone marrow null lymphocytes were intermediate in their size between small lymphocytes and lymphoblasts. The presence of a lot of short microvilli on null lymphocytes was demonstrated by scanning electron microscopic examinations. The bone marrow null lymphocytes had no cytoplasmic Ig. When the bone marrow null lymphocytes were mixedlymphocyte-cultured with allogeneic T lymphocytes and then stimulated with PWM, there developed Ig producing cells. Differences between pre-B cells and bone marrow null lymphocytes were discussed.human bone marrow lymphocyte population; bone marrow null lymphocyte; development of Ig producing cells; pre-B cellThe nature of the human bone marrow lymphocyte population remains still to be clarified. In mice the distribution of lymphocytes bearing mouse specific B lymphocyte antigen (MBLA), surface membrane Immunoglobulin (SmIg), complement receptor and Fc receptor is 25 N 45%, 5 N 15%, 5 N 10% and 10 N 15%, respectively (Greaves et al. 1973). A small portion of mice bone marrow lymphocytes is known to be T lymphocytes. According to Douglas (1976), more than 75% of human bone marrow lymphocytes are B lymphocytes and less than 25% are T lymphocytes.However, little is known about the exact distribution of the human bone marrow lymphocytes population. The population of normal human bone marrow lymphocytes was determined and the morphology of bone marrow null lymphocytes was studied by transmission and scanning electron microscopy.The in vitro development of Ig producing cells from the human bone marrow lymphocytes under the regulation of T lymphocytes by the PWM stimulation was also examined.

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Separation of Bone Marrow Cells by Sedimentation at Unit Gravity

Cited by 127 publications

References 4 publications

Separation of Somatotrophs from the Rat Adenohypophysis by Velocity and Density Gradient Centrifugation

Separation of Somatotrophs from the Rat Adenohypophysis by Velocity and Density Gradient Centrifugation

Enhanced Erythrocyte Suspension Layer Stability Achieved by Surface Tension Lowering Additives

The in vitro development of immunoglobulin producing cells from the human bone marrow null lymphocyte.

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