Separation of Biological Entities from Human Blood by Using Magnetic Nanocomposites Obtained from Zeolite Precursors

Abstract

In this work, three novel magnetic metal–ceramic nanocomposites were obtained by thermally treating Fe-exchanged zeolites (either A or X) under reducing atmosphere at relatively mild temperatures (750–800 °C). The so-obtained materials were thoroughly characterized from the point of view of their physico-chemical properties and, then, used as magnetic adsorbents in the separation of the target gene factors V and RNASE and of the Staphylococcus aureus bacteria DNA from human blood. Such results were compared with those obtained by using a top ranking commercial separation system (namely, SiMAG-N-DNA by Chemicell). The results obtained by using the novel magnetic adsorbents were similar to (or even better than) those obtained by using the commercial system, both during manual and automated separations, provided that a proper protocol was adopted. Particularly, the novel magnetic adsorbents showed high sensitivity during tests performed with small volumes of blood. Finally, the feasible production of such magnetic adsorbents by an industrial process was envisaged as well.

Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p

“… 16 Pathogen culture is often used to diagnose or evaluate the prognosis of sepsis, but its false positive is high and the diagnostic efficiency is undesirable. 17 It has been found that many kinds of lncRNAs are involved in the regulation of inflammation and the immune process, which can be used in the discrimination of sepsis. 18 …”

“…16 Pathogen culture is often used to diagnose or evaluate the prognosis of sepsis, but its false positive is high and the diagnostic efficiency is undesirable. 17 It has been found that many kinds of lncRNAs are involved in the regulation of inflammation and the immune process, which can be used in the discrimination of sepsis. 18 With the development of gene research technology, it has been found that lncRNAs have various functions and play crucial roles in many physiological processes and diseases.…”

Fabrication, Microstructure and Colloidal Stability of Humic Acids Loaded Fe3O4/APTES Nanosorbents for Environmental Applications

“…Adsorption of polyanionic HA on iron oxide nanoparticles and amino-silica-coated induces a zeta potential reversal in the acidic region; the negative charges on nanoparticles become gradually dominant. The reach of a negative surface over the entire pH (3)(4)(5)(6)(7)(8)(9)(10) interval indicates a complete coverage of the surface with humic acids. The maximum value of the negative zeta potential that can be achieved with an increase in the concentration of HA is 40 mV.…”

“…The magnetite nanoparticles (MNPs) have a wide range of applications, from the magnetic separation of technical media [1][2][3] to the preparation of polymer suspensions for biomedicine [4][5][6][7][8] and the life sciences [9]. The modification of MNPs with different species of stabilizing agents leads to producing monodispersed nanopowders and to supplying specific interactions with the molecules due to appropriate functional groups needed in various applications.…”

Fabrication of chitosan-coated mixed spinel ferrite integrated with graphene oxide (GO) for magnetic extraction of viral RNA for potential detection of SARS-CoV-2

“…Magnetic separation (MS) is an increasingly significant technique for molecular biology. The MS technique is productive for purification at the commercial level [ 6 , 7 ]. Recently, target-specific magnetic nanocomposites (MNCs) have been developed which in conjugation with an adequate buffer system could make speedy and efficient purification.…”