Separation of apoptotic cells using a microfluidic device

Son, Oh Taek; Kim, Kunhong; Kim, Sang Hyeob; Maeng, Sunglyul; Jung, Hyo Il

doi:10.1007/s10529-007-9451-1

Cited by 17 publications

(5 citation statements)

References 13 publications

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“… 15 , 16 Meanwhile, the magnetophoresis of live cells has found widespread application, ranging from basic quantification of nanomaterials 17 to the detection and isolation of bacteria in water. 18 , 19 The manipulation of whole eukaryotic cells using magnetic nanostructures is especially powerful in microfluidic structures, where local magnetic forces can be applied efficiently, 20 , 21 and different cell types can be sorted, 22 , 23 such as apoptotic cells 24 or specific cancer cells. 25 , 26 In order to assess the uptake mechanisms used by the cells, 17 , 27 and to find out about physiological consequences 28 and potential cytotoxicity 29 during the modification of eukaryotic cells with magnetic nanomaterials, it is necessary to understand the interaction of magnetic nanostructures with the cellular biomolecules and their behavior in the cell.…”

Section: Introductionmentioning

confidence: 99%

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Büchner

Drescher

Merk

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Büchner

Drescher

Merk

et al. 2016

Analyst

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“…Models of microfluidic magneto-affinity separation are limited in the current literature. Past theoretical models may be divided into two types: capture of magnetic particles only (Chronis et al, 2001;Fukui et al, 2004;Hoffmann and Franzreb, 2004;Warnke, 2003), and capture of cells Frazier, 2005, 2006;Kim et al, 2007;Nan Xia et al, 2006). All previous theoretical studies incorporate basic magnetic and hydrodynamic forces.…”

Section: Introductionmentioning

confidence: 99%

Wall effects in continuous microfluidic magneto‐affinity cell separation

Zhang

Palaniapan

et al. 2010

Biotech & Bioengineering

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Continuous microfluidic magneto-affinity cell separator combines unique microscale flow phenomenon with advantageous nanobead properties, to isolate cells with high specificity. Owing to the comparable size of the cell-bead complexes and the microchannels, the walls of the microchannel exert a strong influence on the separation of cells by this method. We present a theoretical and experimental study that provides a quantitative description of hydrodynamic wall interactions and wall rolling velocity of cells. A transient convection model describes the transport of cells in two-phase microfluidic flow under the influence of an external magnetic field. Transport of cells along the microchannel walls is also considered via an additional equation. Results show the variation of cell flux in the fluid phases and the wall as a function of a dimensionless parameter arising in the equations. Our results suggest that conditions may be optimized to maximize cell separation while minimizing contact with the wall surfaces. Experimentally measured cell rolling velocities on the wall indicate the presence of other near-wall forces in addition to fluid shear forces. Separation of a human colon carcinoma cell line from a mixture of red blood cells, with folic acid conjugated 1 microm and 200 nm beads, is reported.

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“…Cell separation technology using microfluidic devices has emerged as an efficient technology that allows the user to purify target cells from a variety of environmental and biological samples, which can then be isolated and collected for downstream testing. Numerous techniques have been developed to achieve this purpose, including magnetophoretic , , hydrodynamic , , and acoustic methods. Dielectrophoresis (DEP), the translational motion of charge neutral matter caused by polarization effects in nonuniform electric fields , is a recently emerging technique that can separate cells in microfluidic devices rapidly because low voltages produce significant and contactless forces on cells without any modification or labeling .…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Separation of Airborne Microbes and Dust Particles Using a Microfluidic Channel for Real-Time Bioaerosol Monitoring

Moon

Nam

Park

et al. 2009

Environ. Sci. Technol.

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Airborne microbes such as fungi, bacteria, and viruses are a threat to public health. Robust and real-time detection systems are necessary to prevent and control such dangerous biological particles in public places and dwellings. For direct and real-time detection of airborne microbes, samples must be collected and typically resuspended in liquid prior to detection; however, environmental particles such as dust are also trapped in such samples. Therefore, the isolation of target bacteria or a selective collection of microbes from unwanted nonbiological particles prior to detection is of great importance. Dielectrophoresis (DEP), the translational motion of charge neutral matter in nonuniform electric fields, is an emerging technique that can rapidly separate biological particles in microfluidics because low voltages produce significant and contactless forces on particles without any modification or labeling. In this paper, we propose a new method for the separation of airborne microbes using DEP with a simple and novel curved electrode design for separating bacteria in a solution containing beads or dust that are taken from an airborne environmental sample. Using this method, we successfully isolated 90% of the airborne bacterium Micrococcus luteus from a mixture of bacteria and dust using a microfluidic device, consisting of novel curved electrodes that attract bacteria and repel or leave dust particles. As there has been little research on analyzing environmental samples using microfluidics and DEP, this work describes a novel strategy for a rapid and direct bioaerosol monitoring system.

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Separation of apoptotic cells using a microfluidic device

Cited by 17 publications

References 13 publications

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Wall effects in continuous microfluidic magneto‐affinity cell separation

Dielectrophoretic Separation of Airborne Microbes and Dust Particles Using a Microfluidic Channel for Real-Time Bioaerosol Monitoring

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