Separation of antigen‐specific lymphocytes. A new general method of releasing cells bound to nylon mesh

Kiefer, H.

doi:10.1002/eji.1830050909

Cited by 25 publications

(2 citation statements)

References 23 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present paper we describe such a method. As shown by Rutishauser and Edelman [5] and by Kiefer [ 6 ] , antigen-binding T and B lymphocytes specifically adhere to antigen-coated nylon. In Kiefer's procedure [6, 71 specific adsorption occurs at low temperature.…”

Section: Introductionmentioning

confidence: 87%

Specific enrichment of antigen‐binding receptors from sensitized murine lymphocytes

Krawinkel

Rajewsky

1976

Eur J Immunol

View full text Add to dashboard Cite

Splenic T and B lymphocytes from sensitized mice were adsorbed to antigen-coated nylon discs and realesed from the discs by temperature shift as described by Kiefer, H., (Eur. J. Immunol. 1973. 3: 181 and 1975. 5:624). After cell release, lymphocyte-derived antigen-binding material could be recovered from the discs. A major fraction of the activity (70 -80%) binds to anti-immunoglobulin immunosorbents. A minor fraction does not detectably cross-react with gamma, mu, alpha and kappa immunoglobulin poly-peptide chains. When filtrated through Sephadex G-200, the bulk of activity of both fractions elutes in the region of 7 S serum antibody. However, as demonstrated for (4-hydroxy-3-nitro-phenyl)acetyl (NP)-binding material from C57BL/6 lymphocytes, the average affinity for antigen of the two fractions is drastically lower than that of humoral antibody, and the average affinity of the minor fraction is lower than that of the major fraction. The fraction of lymphocyte-derived antigen-binding material that does not adsorb to insolubilized anti-immunoglobulin serum was found to be proportional to the fraction of T lymphocytes in the input cell population and may therefore represent antigen-binding T lymphocyte surface receptors.

show abstract

Section: Introductionmentioning

confidence: 87%

Specific enrichment of antigen‐binding receptors from sensitized murine lymphocytes

Krawinkel

Rajewsky

1976

Eur J Immunol

View full text Add to dashboard Cite

show abstract

“…Thiol chemistry can thus be targeted to the C-terminus, which allowed us to reversibly immobilize LaP-1 to magnetic amine-coated Dynabeads using an N-Succinimidyl 3-(2-pyridyldithio)-propionate (SPDP) crosslinker with a 12 unit PEG spacer. This produces a disulfide-containing crosslink to the bead, which is easily cleavable in mild reducing conditions [23; 24]. Beads with LaP-1 immobilized in this manner were tested in isolations of RNA polymerase, Nup84, and Cdc33 as before.…”

mentioning

confidence: 99%

Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes

Fridy

Thompson

Ketaren

et al. 2015

Analytical Biochemistry

View full text Add to dashboard Cite

In addition to its high affinity for antibody Fc domains, staphylococcal Protein A has been shown to bind certain Fab domains. We investigated this in order to develop a small, recombinant Protein A-binding alternative to IgG from nanobodies, single-domain antibodies derived from a camelid variant IgG’s variable region. We engineered a nanobody with affinity solely for Protein A, as well as a dimerized version of higher affinity for typical multi-domain Protein A constructs. As this recombinant nanobody can be immobilized using a cleavable crosslinker, it has proven suitable for the isolation and mild elution of protein complexes in native conditions.

show abstract

Suppressor cells in rabbit peripheral blood

Luzzati¹,

Lafleur²

1976

Eur J Immunol

View full text Add to dashboard Cite

Rabbit peripheral blood leukocytes (PBL) added to cultures of autologous spleen cells, primed in vivo to sheep red cells, are able to suppress with high efficacy the secondary in vitro response of the spleen cells to that antigen. Removal of nylon wool adherent cells from PBL abolishes the suppressive effect. When the PBL are fractionated by velocity sedimentation the suppressor cells can be separated from the responding cells. The circulating lymphocytes, freed from the inhibiting effect, either by nylon wool absorption or by velocity sedimentation fractionation, are able to give a strong secondary in vitro anti-SRC response, in which the long latency period, usually observed when PBL are stimulated with antigen in culture, is abolished or at least reduced. The suppressor effect present in the PBL is not due to granulocytes, platelets or erythrocytes.

show abstract

Separation of antigen‐specific lymphocytes. A new general method of releasing cells bound to nylon mesh

Cited by 25 publications

References 23 publications

Specific enrichment of antigen‐binding receptors from sensitized murine lymphocytes

Specific enrichment of antigen‐binding receptors from sensitized murine lymphocytes

Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes

Suppressor cells in rabbit peripheral blood

Contact Info

Product

Resources

About