Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

Chen, Zuliang; Warren, Charles R.; Adams, Mark A.

doi:10.1007/bf02490562

Cited by 18 publications

(12 citation statements)

References 28 publications

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“…In a typical Arabidopsis phloem sample the major amino acids were Glu, Asp, and Gln, which together accounted for 65.47% of the total amino acids, and the essential amino acids (Arg, His, Val, Ile, Leu, Lys, Met, Phe, Thr) which accounted for 16.27% of the total amino acids. These findings are comparable to those reported in the literature for phloem sap obtained from other plants [18,19,39,40].…”

Section: Application To the Phloem Sap And Honeydew Analysissupporting

confidence: 94%

Amino acid analysis by micellar electrokinetic chromatography with laser‐induced fluorescence detection: Application to nanolitre‐volume biological samples from Arabidopsis thaliana and Myzus persicae

et al. 2005

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Amino acids were derivatised with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazol (NBD-F), separated by micellar electrokinetic chromatography (MEKC), and detected by argon-ion (488 nm) laser-induced fluorescence. The optimised MEKC background electrolyte conditions were: 40 mM sodium cholate, 5 mM beta-cyclodextrin in 20 mM aqueous borate buffer, pH 9.1, with 7% v/v acetonitrile. Using these conditions, 19 amino acids were separated within 17 min. The limits of detection were in the range of 7.6-42.2 pmol/mL and limits of quantitation from 0.05-0.14 nmol/mL. The method was systematically validated for injection volume error, migration time variation, calibration linearity, accuracy, precision, and recovery. Nanolitre volume samples of phloem sap of individual sieve element cells from the plant Arabidopsis thaliana and honeydew from the aphid Myzus persicae were directly analysed with this method. Quantitative amino acid concentrations in these two biological matrices were profiled for the first time. This method is particularly important because it allows the complete profile of the amino acids obtained from individual phloem elements, allowing cell to cell and plant to plant variation to be quantified, which to date has not been possible with Arabidopsis thaliana.

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Section: Application To the Phloem Sap And Honeydew Analysissupporting

confidence: 94%

Amino acid analysis by micellar electrokinetic chromatography with laser‐induced fluorescence detection: Application to nanolitre‐volume biological samples from Arabidopsis thaliana and Myzus persicae

et al. 2005

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“…pK a values of underivatized amino acids, deduced from the pH values, as well as the corresponding electrophoretic mobilities were measured using a specially developed program [10]. Indirect UV detection was also used for the analysis of phospho amino acids [11] and amino acids in plants [12] (Fig. 1).…”

Section: Uv Detectionmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electrophoresis

et al. 2001

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“…As most of the native amino acids absorb only slightly or not at all ultraviolet light (UV), different detection approaches were used in combination with the CZE and MEKC separation techniques. UV and fluorescence or laser-induced fluorescence (LIF) detection after a suitable precolumn, postcolumn and on-column derivatization with a UV chromophore [8][9][10][11][12][13][14] and fluorophore [7,[15][16][17][18], respectively, direct UV detection at a very low wavelength [19,20], indirect UV detection [21], electrochemical detection [22,23], mass spectrometry [24,25], and some other detection techniques [26,27] were applied to the analyses of amino acids.…”

Section: Introductionmentioning

confidence: 99%

Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection

Coufal¹,

et al. 2003

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Twenty underivatized essential amino acids were separated using capillary zone electrophoresis and consequently detected with contactless conductivity detection (CCD). A simple acidic background electrolyte (BGE) containing 2.3 M acetic acid and 0.1% w/w hydroxyethylcellulose (HEC) allowed the electrophoretic separation and sensitive detection of all 20 essential amino acids in their underivatized cationic form. The addition of HEC to the BGE suppressed both, electroosmotic flow and analyte adsorption on the capillary surface resulting in an excellent migration time reproducibility and a very good analyte peak symmetry. Additionally, the HEC addition significantly reduced the noise and long-term fluctuations of the CCD baseline. The optimized electrophoretic separation method together with the CCD was proved to be a powerful technique for determination of amino acid profiles in various natural samples, like beer, yeast, urine, saliva, and herb extracts.

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Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

Cited by 18 publications

References 28 publications

Amino acid analysis by micellar electrokinetic chromatography with laser‐induced fluorescence detection: Application to nanolitre‐volume biological samples from Arabidopsis thaliana and Myzus persicae

Amino acid analysis by micellar electrokinetic chromatography with laser‐induced fluorescence detection: Application to nanolitre‐volume biological samples from Arabidopsis thaliana and Myzus persicae

Recent advances in amino acid analysis by capillary electrophoresis

Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection

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