Separation of amino acids by high performance liquid chromatography

Bayer, Ernst; Grom, Edgar; Kalteneger, B; Uhmann, Rainer

doi:10.1021/ac50002a010

Cited by 196 publications

(41 citation statements)

References 19 publications

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“…Several pre-column derivatization methods affording sub-nanomole sensitivity are available [10][11][12][13][14][15][16][17]. Each of them, however, has some intrinsic defect.…”

Section: Comparison Of the Dabs-c1 Methods With Other Pre-cohlmn Derivmentioning

confidence: 99%

High‐sensitivity amino acid analysis: measurement of amino acid neurotransmitter in mouse brain

Chang¹,

Martin²,

Bernasconi³

et al. 1981

FEBS Letters

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“…Several pre-column derivatization methods affording sub-nanomole sensitivity are available [10][11][12][13][14][15][16][17]. Each of them, however, has some intrinsic defect.…”

Section: Comparison Of the Dabs-c1 Methods With Other Pre-cohlmn Derivmentioning

confidence: 99%

High‐sensitivity amino acid analysis: measurement of amino acid neurotransmitter in mouse brain

Chang¹,

Martin²,

Bernasconi³

et al. 1981

FEBS Letters

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“…Sample processing, two-dimensional gel electrophoresis, gel processing, and fluorography were carried out as described (32 (33), and the resulting fractions were analyzed by two-dimensional TLC on cellulose plates (34) and by dansylation followed by HPLC (35).…”

mentioning

confidence: 99%

Methylation of elongation factor 1 alpha from the fungus Mucor.

Hiatt

Garcia

Merrick

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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A basic protein from the dimorphic fungus Mucor racemosus, found to be highly methylated, is shown to be protein synthesis elongation factor la. This protein is the most abundant protein in hyphal cells but is less abundant in yeast cells. It is posttranslationally methylated with the formation of mono-, di-, and trimethyllysine at as many as 16 sites. Nearly 20% of the 44 lysine residues of elongation factor la from mycelia are modified while those from sporangiospores are virtually unmethylated.Mucor racemosus is a dimorphic fungus that exists in either yeast or hyphal forms. These changes in morphology are accompanied by changes in the translational system, evidenced by an acceleration in the rate of peptide bond formation (1, 2) and by a change in the level of phosphorylation of a ribosomal protein (3). In the course of studying posttranslational modifications in the fungus, we resolved several methylated peptides by using two-dimensional polyacrylamide gel electrophoresis. We have now identified one of these peptides as the a subunit of protein synthesis elongation factor 1 (EF-la), the functional equivalent of prokaryotic elongation factor Tu (EF-Tu) (4). EFTu from Salmonella typhimurium and Escherichia coli is methylated (5, 6), and that in E. coli contains a single methyllysine at position 56.In certain proteins, acidic amino acids are methylated by a reversible carboxymethylation reaction, examples of which have been shown to play roles in bacterial chemotaxis (7-9), leukocyte chemotaxis (10-12), and hormone secretion (13). The methylation of basic amino acids occurs at the E-amino group of lysine, the guanidinium group of arginine, or the imidazole group of histidine (14). Although these reactions show a high degree of specificity, there has been no clear functional role assigned to the presence ofmethylated basic amino acids. Methylated basic amino acids occur in histones (15,16,17)

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“…-64- et sle, 1962;Dejong et a1., 1982 ;Tapuh1 et a1., 1981;Parris and Gallel1i, 1984 ;Mac Nicol , 1978;Bayer et al, 1976 Recovery of free amino acids in the aqueous alcohol fraction was determined by two different techniques. In the first procedure, aliquots of an isotopically labeled mixture of amino f:lcids were added to a combusted Whatman GF/F glas8 fiber filter (Cllt to a diameter of 13 mm and -73-o combuated overnight at 500 C).…”

Section: Coapariaon Of Subcellular Fractionation Proce~mentioning

confidence: 99%

Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter

Lohrenz¹

1985

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Separation of amino acids by high performance liquid chromatography

Cited by 196 publications

References 19 publications

High‐sensitivity amino acid analysis: measurement of amino acid neurotransmitter in mouse brain

High‐sensitivity amino acid analysis: measurement of amino acid neurotransmitter in mouse brain

Methylation of elongation factor 1 alpha from the fungus Mucor.

Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter

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