Separation of amino acids and antibiotics by narrow-bore and normal-bore high-performance liquid chromatography with pre-column derivatization

Fiedler, H.-P.; Plaga, Armin

doi:10.1016/s0021-9673(01)94600-x

Cited by 16 publications

(5 citation statements)

References 8 publications

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“…Free and total amino acids were determined via high‐performance liquid chromatography by an automated pre‐column derivatisation with o‐phthalaldehyde‐2‐mercaptoethanol reagent and UV detection at 338 nm as described by (Fiedler and Plaga, 1987). The concentration of the bound amino acids was calculated by subtraction of the concentration of the free amino acids from the concentration of the total amino acids.…”

Section: Methodsmentioning

confidence: 99%

Tuber‐specific cphA expression to enhance cyanophycin production in potatoes

Hühns

Neumann

Hausmann

et al. 2009

Plant Biotechnology Journal

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SummaryThe production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to increase polymer production, we restricted cyanophycin accumulation to the potato tubers by using the cyanophycin synthetase gene (cphA Te ) from Thermosynechococcus elongatus BP-1, which is under the control of the tuber-specific class 1 promoter (B33).Tuber-specific cytosolic (pB33-cphA Te ) as well as tuber-specific plastidic (pB33-PsbYcphA Te ) expression resulted in significant polymer accumulation solely in the tubers.In plants transformed with pB33-cphA Te , both cyanophycin synthetase and cyanophycin were detected in the cytoplasm leading to an increase up to 2.3% cyanophycin of dry weight and resulting in small and deformed tubers. In B33-PsbY-cphA Te tubers, cyanophycin synthetase and cyanophycin were exclusively found in amyloplasts leading to a cyanophycin accumulation up to 7.5% of dry weight. These tubers were normal in size, some clones showed reduced tuber yield and sometimes exhibited brown sunken staining starting at tubers navel. During a storage period over of 32 weeks of one selected clone, the cyanophycin content was stable in B33-PsbYcphA Te tubers but the stress symptoms increased. However, all tubers were able to germinate. Nitrogen fertilization in the greenhouse led not to an increased cyanophycin yield, slightly reduced protein content, decreased starch content, and changes in the amounts of bound and free arginine and aspartate, as compared with control tubers were observed.

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Section: Methodsmentioning

confidence: 99%

Tuber‐specific cphA expression to enhance cyanophycin production in potatoes

Hühns

Neumann

Hausmann

et al. 2009

Plant Biotechnology Journal

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“…The procedure and equipment was identical with that previously described [16]. The absolute configuration of the amino acids was determined by gas chromatography of the N-trifluoroacetylated, n-propyl ester derivatives, on a Duran glass capillary column coated with Chirasil Val [17] (Siemens Sichromat 1, Siemens Autosampler 200, C. A.T. Autoderivat 100, Spectra Physics SP 4270 Data System).…”

Section: Amino Acid Analysis and Determination Of Enantiomenmentioning

confidence: 99%

Staphyloferrin A: a structurally new siderophore from staphylococci

Konetschny-Rapp

Jung

Meiwes

et al. 1990

European Journal of Biochemistry

139

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Two ferric ion-binding compounds, designated staphyloferrin A and B, were detected in the culture filtrates of staphylococci grown under iron-deficient conditions. Staphyloferrin A was isolated from cultures of Staphylococcus hyicus DSM 20459. The structural elucidation of this highly hydrophilic, acid-labile compound revealed a novel siderophore, N 2 , N 5 -di-(l-oxo-3-hydroxy-3,4-dicarboxybutyl)-~-ornithine, which consists of one ornithine and two citric acid residues linked by two amide bonds. The two citric acid components of staphyloferrin A provide two tridentate pendant ligands, comprising of a /I-hydroxy, P-carboxy-substituted carboxylic acid derivative, for octahedral metal chelation. The CD spectrum of the staphyloferrin A ferric complex indicates a predominant A configuration about the ferric ion center. The uptake of ferric staphyloferrin A by S. hyicus obeys MichaelisMenten kinetics ( K , = 0.246 pM; v,,, = 82 pmol . mg-. min-I), indicating active transport of this siderophore.The staphyloferrin A transport system is different from that of the ferrioxamines as shown by an antagonism test. Production of staphyloferrin A is strongly iron-dependent and is stimulated by supplementation of the medium with either D-or L-ornithine. ~~-[S-'~C]ornithine was incorporated into staphyloferrin A, demonstrating that ornithine is an intermediate in staphyloferrin A biosynthesis.Iron, the fourth most abundant element of the earths crust, is required by all aerobic microorganisms and plays an important role in membrane-bound electron-transport chains as well as in cytoplasmic redox enzymes. In an aerobic environment iron is present in the Fe(II1) oxidized form, which polymerizes into insoluble ferric oxyhydroxides under physiological conditions [l, 21. In mammals, iron is bound to proteins such as lactoferrin and transferrin, and is therefore inaccessible, by direct means, to microorganisms [3]. Thus, microorganisms have developed different strategies to provide themselves with iron. The most common and well-investigated strategy is the high-affinity Fe( 111) uptake via siderophores. During iron deficiency, microorganisms excrete low-molecular-mass iron(II1)-chelating compounds (siderophores) in their iron-free form. After ferric ion chelation, the complexes are actively taken up by specific transport systems [4]. Several hundred siderophores from prokaryotes and eukaryotes are now known, which can structurally be assigned to the major classes of hydroxamate-and catecholate-type siderophores.

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“…As most amino acids lack UV absorbance in 220-254 nm range, general method employs derivatization agent to transform the analytes into derivatives that can be separated and determinated more easily (Knapp 1979;Fan et al 1998;Kőrös et al 2008). It was well accepted that typical amino acid derivatization reagents include o-phthalaldehyde (Zacharis et al 2006;Pereira et al 2008), phenyl isothiocyanate (Fiedler and Plaga 1987;Fernández-Fígares et al 2004), 9-fluorenylmethyloxycarbonyl chloride (Einarsson et al 1983;López-Cervantes et al 2006;Lozanov et al 2007), dansyl chloride (Naval et al 2006), 6-aminoquinolyl-N-hydroxysuccinimdyl carbaminate (Bosch et al 2006;Li et al 2007), and 2,4-dinitrofluorobenzene (Gioia et al 2007). Specific advantages and limitations in their applications have also been reported (Gatti et al 2001;Todoroki et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

Hou

Cao

et al. 2011

Food Anal. Methods

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A novel method was studied for determination of 20 free amino acids in asparagus tin by high-performance liquid chromatography with pre-column derivatization. Derivatization of the samples was performed with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF), and the SPE cartridge was used for purification and enrichment of the analytes. The derivatization conditions and the influence of elution composition on the separation were investigated. The reaction of amino acids with CNBF was completed in pH 9.0 borate buffer. The separation of amino acids was achieved at room temperature within 60 min by gradient elution mode with triethylamine in mobile phase, and the flow rate is 0.32 mL min −1 constantly. The method correlation coefficient was from 0.9975 to 1.0000 in concentrations ranging from 20 to 2000 μmol L −1 , except asparagine (from 100 to 10000 μmol L −1 ). The detection limits of amino acids were from 1.2 to 6.0 μmol L −1 , with a signal-to-noise ratio of three times. The calculated recoveries of the proposed method were from 81.4% to 109.4%, and relative standard deviations were 0.48-3.94% in application to the quantitative determination of free amino acids in asparagus tin samples. The present method is reliable and sensitive that allows fast analysis of free amino acids in asparagus tin, which makes it suitable for further study of free amino acids in other asparagus foods.

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Separation of amino acids and antibiotics by narrow-bore and normal-bore high-performance liquid chromatography with pre-column derivatization

Cited by 16 publications

References 8 publications

Tuber‐specific cphA expression to enhance cyanophycin production in potatoes

Tuber‐specific cphA expression to enhance cyanophycin production in potatoes

Staphyloferrin A: a structurally new siderophore from staphylococci

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

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