Separation of 3‐Biphenylcarboxylic Acid and 4‐Biphenylcarboxylic Acid Isomers with Chromatographic Column of Solid β‐Cyclodextrin

Abstract

The solid β-cyclodextrin (β-CD) was selected as the separating medium of chromatographic column to separate the isomers, 3-biphenylcarboxylic (3-BPCA) and 4-biphenylcarboxylic (4-BPCA). Separation capability for different solvents (ethanol, ethyl acetate, chloroform, toluene, and hexane) was investigated, and was assessed based on the separation factor (S). The results showed that hexane was considered as the best solvent due to the high separation factor of 1.525 and a sharper eluent peak compared to the other solvents. Ethanol could dissociate 3-BPCA and 4-BPCA from β-CD completely due to its strong polarity, so ethanol was selected as the reverse solvent. This study provided satisfactory results on the purity and yield for the separation of 3-BPCA and 4-BPCA.

Measurement and Correlation of the Solubility of 4-Biphenylcarboxylic Acid in Nine Pure and Binary Mixed Organic Solvents from T = 293.15 to 328.15 K

“…It can also be used as an intermediate in pharmaceutical synthesis, such as the preparation of biphenyldiazole derivatives. The results of in vitro testing of antibacterial activity show that the compound has strong antifungal and antibacterial activity. − …”

“…The crystals are taken out and reacted with potassium permanganate below 90 °C, filtered, and neutralized with hydrochloric acid to a pH of 2, to obtain a crude product. The product is recrystallized with ethanol and refined by acid–base precipitation to obtain 4-biphenylcarboxylic acid. − …”