Separation-Free Spectrophotometric Platforms for Rapid Assessment of Combined Antiplatelet Therapy in Complex Matrices

Abstract

Aim: To develop simple and rapid UV-spectrophotometric platforms for the simultaneous quantification of a binary mixture containing clopidogrel bisulphate (CPS) and aspirin (ASP) in complex matrices without prior separation. Experimental: Five mathematical models namely ratio-difference method, mean centering of the ratio spectra, dual wavelength, induced dual wavelength and H-Point Standard Addition method, were utilized for resolving spectral overlap by mathematical processing of ratio and zero-order absorption spectra. Analytes were extracted from tested matrices (whole blood, pharmaceutical formulations and dissolution media buffer) and quantified using the proposed methods. The methods were validated according to ICH guidelines. Results: The developed methods demonstrated limits of detection ranging from 0.67 to 1.09 μg/ml-1 for CPS and 0.49 to 0.71 μg.ml-1 for ASP. All proposed methods allowed for reliable determination of CPS and ASP in complex matrices within reported reference ranges, indicating their potential application for therapeutic drug monitoring and quality control testing.

Greenness‐by‐design approach for developing novel UV spectrophotometric methodologies resolving a quaternary overlapping mixture

“…[7][8][9][10][11] Analytical method development is a multistep process involving several trial and error cycles bearing within a huge environmental burden due to the variety of the utilized solvents and reagents. [12,13] Early efforts to turn the method development phase greener were by excluding the use of solvents and reagents of the highest ecological impact. [14] These efforts lead to limitations in the exploratory space available for the researchers to develop highly sensitive and selective analytical methodologies, especially those requiring preliminary sample preparations.…”

Ecological spectroscopic methodologies for quantifying co-administered drugs in human plasma by photochemical quantum mechanical simulation

“…The development of future fixed dose pharmaceutical combinations requires powerful and simple analytical methods that are cost effective [ [13] , [14] , [15] , [16] ]. In a previous study, we demonstrated the effectiveness of the greenness by design approach (GbD) in the swift development of eco-friendly analytical methodologies for resolving and determining complex pharmaceutical mixtures.…”

Charge transfer complex-based spectrophotometric analysis of famotidine in pure and pharmaceutical dosage forms

“…Additionally, the flow injection analysis method using spectrophotometry has been utilised 9 , 10 . Electrochemical methods such as the voltammetric method 11 – 13 and the polarography method have been explored, along with the use of ion-selective electrodes 14 , 15 .…”