Separation by Ethanol of Common and Somatic Antigens of Enterobacteriaceae

Suzuki, Takashi; Gorzynski, E. A.; Neter, Erwin

doi:10.1128/jb.88.5.1240-1243.1964

Cited by 73 publications

(48 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…coii antibody. A pooled O antigen was prepared from a boiled bacterial suspension [14] of six common enteric strains of E. coli (075:HNM, 0101:HNM, 0123:H6, 0141:H4, 018ac:H7, 0149:H10). IgA antibody against this antigen preparation was measured by means of an adaptation of an enzyme-linked immunosorbent assay (ELISA).…”

Section: Methodsmentioning

confidence: 99%

The Development of IgA‐Specific Antibodies to Escherichia coli O Antigen in Children

et al. 1987

View full text Add to dashboard Cite

One hundred and sixty-five infants were studied longitudinally from birth to 5 years of age. One hundred and twenty-three school-age children and 27 adults were examined cross-sectionally. Total salivary IgA levels and IgA antibodies against Escherichia coli O antigens were measured. Total IgA levels were low (less than 20 mg/l) from birth to 4 years of age. At 5 years of age there was a dramatic increase in the total IgA level (geometric mean = 100.7 mg/l), after which the levels fell to values similar to those observed in adults (adult geometric mean = 53.2 mg/l). Low levels of IgA-specific E. coli antibodies were observed for the first 4 years of life (less than 1.0 ELISA units). There was a gradual increase in specific antibodies between 5 and 9 years of age (geometric men at 9 years = 4.66 ELISA units) to levels similar to those observed in adults (adult geometric mean = 8.20 ELISA units). It is suggested that the patterns of development for these variables reflect a balance between antigenic exposure and immune control mechanisms.

show abstract

Section: Methodsmentioning

confidence: 99%

The Development of IgA‐Specific Antibodies to Escherichia coli O Antigen in Children

et al. 1987

View full text Add to dashboard Cite

show abstract

“…strains as before (14). The common enterobacterial antigen (CA) was prepared from S. typhimurium (14) using the technique described by Suruki et al (21). The heat extracts and the CA extract were dialysed and lyophilized.…”

Section: Preparation Of Bacterial Extractsmentioning

confidence: 99%

HUMAN SERUM ANTIBODIES AGAINST HEAT‐STABLE ANTIGENS FROM YERSINIA ENTEROCOLITICA

Mæland

Digranes

1975

Acta Pathologica Microbiologica Scandinavica Section B Microbio

View full text Add to dashboard Cite

Maeland, J. A. & Digranes, A. Human serum antibodies against heat-stable antigens from Yersinia enterocolitica. Acta path. microbiol. scand. Sect. B, 83: 451-456, 1975. Human sera, 200 from blood donors and five from patients with infection caused by Yersinia enterocolitica serotype 3 (Y.e. 3), were tested by indirect haemagglutination for antibodies against antigens from Y.e. serotypes 3 and 9. Erythrocytes were sensitized using extracts prepared by heating of the bacteria at 100" C for 60 min. All sera tested showed antibody activity against antigen from both Y.e. serotypes. I n blood donors, the titres ranged from 4 to 512, and in patients from 512 to 2048 against the Y . e . 3 extract, from 32 to 256 against the Y.e. 9 extract. The results of inhibition and absorption experiments showed that human antibodies against the common enterobacterial antigen (CA) agglutinated the sensitized erythrocytes and that anti-CA antibody was present in all sera tested, Some blood donor sera and all the patient sera also contained antibodies that could not be inhibited by CA when tested against antigen in the Y.e. 3 extract. Only these sera had the ability to agglutinate heat-treated Y.e. 3 bacteria. Presumably these antibodies were directed against the 0 antigen of the Y.e. 3 strain.

show abstract

“…CA was prepared from S. typhimurium as reported by Suruki et al (14). I n short, heat extract from the bacterium was mixed with absolute ethanol, 1:8.5 (v/v).…”

Section: Preparation Of Bacterial Extractsmentioning

confidence: 99%

“…coli 0 14 (7). Erythrocytes sensitized with CA from S. typhimurium are commonly employed for the demonstration of anti-CA antibodies (7, 14,16).…”

mentioning

confidence: 99%

Common Enterobacterial Antigen in Yersinia Enterocolitica

Ja¹,

Digranes²

1975

Acta Pathologica Microbiologica Scandinavica Section B Microbio

View full text Add to dashboard Cite

Production of the common enterobacterial antigen (CA) by strains of Yersinia enterocolitica (Y. e.) serotypes 3 and 9 (Winblad), by strains of 5 different Y. e. serogroups (Wauters) and various other bacteria was examined. Antibody against CA was raised by immunization of rabbits with E. coli O 14. Extract prepared from S. typhimurium was used for the sensitization of sheep erythrocytes with CA. Absorption and haemagglutination inhibition experiments showed that CA could be detected in heat extracts from all Y.e. strains examined, and in that from Yersinia pseudotuberculosis. CA was not detected in extracts from Pasteurella multocida, Francisella tularensis, Brucella abortus, Acinetobacter calcoaceticus or Pseudomonas aeruginosa. Anti‐CA antibodies could not be demonstrated in serum from rabbits immunized with Y.e. bacteria, but were demonstrated in serum from rabbits immunized with a CA‐containing fraction of the Y.e. extract. The possibility of participation of anti‐CA antibody in indirect haemagglutination tests for detection of antibody to Y.e. O antigens is emphasized.

show abstract

Separation by Ethanol of Common and Somatic Antigens of Enterobacteriaceae

Cited by 73 publications

References 2 publications

The Development of IgA‐Specific Antibodies to Escherichia coli O Antigen in Children

The Development of IgA‐Specific Antibodies to Escherichia coli O Antigen in Children

HUMAN SERUM ANTIBODIES AGAINST HEAT‐STABLE ANTIGENS FROM YERSINIA ENTEROCOLITICA

Common Enterobacterial Antigen in Yersinia Enterocolitica

Contact Info

Product

Resources

About