Separation and study of the range of plasminogen isoforms in patients with prostate cancer

Abstract

Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher pI values compared to isoforms found in the control group.

Circulating proteins as predictive and prognostic biomarkers in breast cancer

“…These techniques have seen a rapid development and several types exist. Indeed, several studies have focused on protein profiling using the matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) MS to determine prostate [ 31 , 32 ], colorectal [ 33 , 34 ] or breast [ 35 , 36 ] cancer biomarkers. As an alternative, surface-enhanced laser desorption/ionisation time-of-flight (SELDI-TOF) MS can be performed.…”