Separation and purification of deoxynivalenol (DON) mycotoxin from wheat culture using a simple two-step silica gel column chromatography

Zhao, Xiumei; Li, Rong-jia; Zhou, Chuanpeng; Zhang, Jie; He, Chenghua; Zheng, Yating; Wu, Weijie; Zhang, Haibin

doi:10.1016/s2095-3119(15)61098-x

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…The obtained ESI-MS, 1 H NMR, and 13 C NMR spectra for the target compound in Fr11 were compared with published literature to clarify the structural characteristics of this compound (Figure 3C). The structural identification of Fr11 based on these data is shown in Supplementary Figures S1-S3, with all of these data (summarized below) supporting the identity of this target compound as 11 The obtained ESI-MS, 1 H NMR, and 13 C NMR spectra for the target compound in Fr11 were compared with published literature to clarify the structural characteristics of this compound (Figure 3C). The structural identification of Fr11 based on these data is shown in Supplementary Figures S1-S3, with all of these data (summarized below) supporting the identity of this target compound as 11…”

Section: Analyses Of the Purity And Structural Characterization Of Po...mentioning

confidence: 76%

“…The complex makeup of S. atrata, as well as its limited solubility, have impeded the isolation of adequately pure compounds from this species to date. Silica-gel column chromatography [11] and recrystallization [12] are traditional approaches to preparing, separating, and purifying NPs. However, they are all limited by low efficiency and low recovery rates [13].…”

Section: Introductionmentioning

confidence: 99%

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Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Fang

et al. 2022

Nutrients

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In this study, a 100 g sample of Saxifraga atrata was processed to separate 1.3 g of 11-O-(4′-O-methylgalloyl)-bergenin (Fr1) after 1 cycle of MCI GEL® CHP20P medium pressure liquid chromatography using methanol/water. Subsequently, COX-2 affinity ultrafiltration coupled with reversed-phase liquid chromatography was successfully used to screen for potential COX-2 ligands in this target fraction (Fr1). After 20 reversed-phase liquid chromatography runs, 74.1 mg of >99% pure 11-O-(4′-O-methylgalloyl)-bergenin (Fr11) was obtained. In addition, the anti-inflammatory activity of 11-O-(4′-O-methylgalloyl)-bergenin was further validated through molecular docking analyses which suggested it was capable of binding strongly to ALOX15, iNOS, ERBB2, SELE, and NF-κB. As such, the AA metabolism, MAPK, and NF-κB signaling pathways were hypothesized to be the main pathways through which 11-O-(4′-O-methylgalloyl)-bergenin regulates inflammatory responses, potentially functioning by reducing pro-inflammatory cytokine production, blocking pro-inflammatory factor binding to cognate receptors and inhibiting the expression of key proteins. In summary, affinity ultrafiltration-HPLC coupling technology can rapidly screen for multi-target bioactive components and when combined with molecular docking analyses, this approach can further elucidate the pharmacological mechanisms of action for these compounds, providing valuable information to guide the further development of new multi-target drugs derived from natural products.

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Section: Analyses Of the Purity And Structural Characterization Of Po...mentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Fang

et al. 2022

Nutrients

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“…It can also cause dysfunctions of the immune, neuroendocrine, and cardiovascular systems [ 26 ]. DON is a polar molecule that can resist at high temperatures, and it is soluble in polar organic solvents [ 27 , 28 ]. It is classified as non-macrocyclic trichothecenes [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

QuEChERS LC–MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices

Pantano

Scala

Olibrio

et al. 2021

IJERPH

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We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB1, AFB2, AFG1, AFG2), deoxynivalenol (DON), fumonisin (FUMO; FB1, FB2, FB3), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method’s performance was assessed by two different proficiencies and tested with 100 real samples.

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“…The extract with the optimal algal inhibition activity was isolated and purified by column chromatography [32]. A column was wet-packed with 100-300 mesh silica for column chromatography and nitrogen gas was used to aid elution.…”

Section: Column Chromatography For Separation and Purificationmentioning

confidence: 99%

Allelochemicals Extracted from Eleocharis dulcis and their Inhibitory Effects on Microcystis aeruginosa

Wen¹,

Shu²,

Chen³

et al. 2017

J Chem Eng Process Technol

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Allelochemicals are considered to be effective substances for controlling the outbreak of harmful cyanobacteria. In this study, Eleocharis dulcis peel fractions with an inhibitory effect on Microcystis aeruginosa were isolated by extraction and purification. The extracted organic compounds were identified by gas chromatography-mass spectrometry (GC-MS), and the allelopathic effect and mechanism underlying the inhibition of M. aeruginosa were explored. The results demonstrated that the exogenous compound, eriodictyol, corresponding to one of the detected flavonoids (C H 12 O 6) could inhibit the growth of M. aeruginosa, indicating that the flavonoid C 15 H 12 O 6 is one of the allelochemicals contained in E. dulcis. Levels of chlorophyll a (Chl-a), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and reduced glutathione (GSH) of cell health in M. aeruginosa were analysed. Our results show that the damage to the antioxidant enzymes of algal cells caused by eriodictyol could be a main contributing factor to the decrease in the number of algal cells and even cell death.

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Separation and purification of deoxynivalenol (DON) mycotoxin from wheat culture using a simple two-step silica gel column chromatography

Cited by 12 publications

References 28 publications

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

QuEChERS LC–MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices

Allelochemicals Extracted from Eleocharis dulcis and their Inhibitory Effects on Microcystis aeruginosa

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