Separation and Purification of both CK-I and CK-II Casein Kinases in Developing Maize Endosperm — Phosphorylation of Native HMG Proteins

Babatsikos, Christos; Yupsanis, Traianos

doi:10.1016/s0176-1617(00)80164-9

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…In plants, six cDNA clones encoding CKI isoforms have been isolated from Arabidopsis thaliana (Klimczak et al ., 1995 and references therein), and at least 21 putative isoform sequences exist in the A. thaliana genome. Additional CKI genes have been isolated from a variety of plant species, including Glycine max (Murray et al ., 1978a), Brassica cauliflora (Murray et al ., 1978b), wheat germ (Rychlik and Zagorski, 1980), Nicotiana tabacum (Erdmann et al ., 1982), maize seedlings (Dobrowolska et al ., 1987), Brassica oleracea (Klimczak and Cashmore, 1993), Psophocarpus tetragonolobus (Mukhopadhyay, 1997), and developing maize endosperm (Babatsikos and Yupsanis, 2000). Again, the nature of the physiological substrates and the physiological functions remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Roles of OsCKI1, a rice casein kinase I, in root development and plant hormone sensitivity

et al. 2003

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SummaryCasein kinases are critical in cell division and differentiation across species. A rice cDNA fragment encoding a putative casein kinase I (CKI) was identi®ed via cDNA macroarray under brassinosteroid (BR) treatment, and a 1939-bp full-length cDNA, OsCKI1, was isolated and found to encode a putative 463-aa protein. RT-PCR and Northern blot analysis indicated that OsCKI1 was constitutively expressed in various rice tissues and upregulated by treatments with BR and abscisic acid (ABA). Enzymatic assay of recombinant OsCKI1 proteins expressed in Escherichia coli showed that the protein was capable of phosphorylating casein. The physiological roles of OsCKI1 were studied through antisense transgenic approaches, and homozygous transgenic plants showed abnormal root development, including fewer lateral and adventitious roots, and shortened primary roots as a result of reduced cell elongation. Treatment of wild-type plants with CKI-7, a speci®c inhibitor of CKI, also con®rmed these functions of OsCKI1. Interestingly, in transgenic and CKI-7-treated plants, exogenously supplied IAA could restore normal root development, and measurement of free IAA content in CKI-de®cient primary and adventitious roots revealed altered auxin content, indicating that OsCKI1 is involved in auxin metabolism or that it may affect auxin levels. Transgenic plants were less sensitive than control plants to ABA or BR treatment during germination, suggesting that OsCKI1 may be involved in various hormone-signaling pathways. OsCKI1-GFP fusion studies revealed the localization of OsCKI1 to the nucleus, suggesting a possible involvement in regulation of gene expression. In OsCKI1-de®cient plants, differential gene expression was investigated using cDNA chip technology, and results indicated that genes related to signal transduction and hormone metabolism were indeed with altered expression.

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Section: Introductionmentioning

confidence: 99%

Roles of OsCKI1, a rice casein kinase I, in root development and plant hormone sensitivity

et al. 2003

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“…Procedures of in situ phosphorylation were performed as described by Babatsikos and Yupsanis (2000).…”

Section: In Situ Phosphorylation After Sds/pagementioning

confidence: 99%

Similarities and differences in the properties of multiple NDP-kinase isoforms of Alyssum murale, Ni2+-accumulator species

Siskos

Yupsani

Symeonidis

et al. 2010

Journal of Plant Physiology

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“…Το ίδιο έχει αναφερθεί και για τις καταλυτικές υπομονάδες (16.5 kDa) της ΝDΡ κινάσης από σπέρματα κριθής (Georgatsos and Fisentzidis 1996). (Nomura et al, 1991;Ohtsuki et al, 1986;Zhang et al, 1995; (Babatsikos and Yupsanis, 2000) ή και παρεμποδιστών στο αρχικό εκχύλισμα. Οι NDPK-A και NDPK-B εμφανίζουν παρόμοια συμπεριφορά μετά από θέρμανση για 10 min σε διάφορες θερμοκρασίες και είναι θερμοάντοχα ένζυμα.…”

Section: ανταπόκριση των Ndp κινασών στο αιθυλένιοunclassified

Καθαρισμός Ιδιότητες Και Εξειδίκευση Ισομορφών Κινασών Των Διφωσφονουκλεοζιτών Από Ενδοσπέρμιο Καλαμποκιού - Μοριακές Προσεγγίσεις

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Separation and Purification of both CK-I and CK-II Casein Kinases in Developing Maize Endosperm — Phosphorylation of Native HMG Proteins

Cited by 5 publications

References 44 publications

Roles of OsCKI1, a rice casein kinase I, in root development and plant hormone sensitivity

Roles of OsCKI1, a rice casein kinase I, in root development and plant hormone sensitivity

Similarities and differences in the properties of multiple NDP-kinase isoforms of Alyssum murale, Ni2+-accumulator species

Καθαρισμός Ιδιότητες Και Εξειδίκευση Ισομορφών Κινασών Των Διφωσφονουκλεοζιτών Από Ενδοσπέρμιο Καλαμποκιού - Μοριακές Προσεγγίσεις

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