Separation and mass spectrometry identification of carotenoid complex from radioresistant bacteria Deinococcus radiodurans

Лысенко, В. С.; Chistyakov, V. A.; Zimakov, D. V.; Soier, V. G.; Сазыкина, М. А.; Сазыкин, И. С.; Krasnov, Vladimir

doi:10.1134/s1061934811130144

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…6 and S2). Furthermore, these findings are consistent with a recent report that questioned the original findings of Lemme et al, [27] and support of our finding that carotenoids other than deinoxanthin can significantly predominate in wild type Drad [45]. …”

Section: Discussionsupporting

confidence: 94%

Untargeted metabolite profiling reveals that nitric oxide bioynthesis is an endogenous modulator of carotenoid biosynthesis in Deinococcus radiodurans and is required for extreme ionizing radiation resistance

Hansler

Chen

et al. 2016

Archives of Biochemistry and Biophysics

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Deinococcus radiodurans (Drad) is the most radioresistant organism known. Although mechanisms that underlie the extreme radioresistance of Drad are incompletely defined, resistance to UV irradiation-induced killing was found to be greatly attenuated in an NO synthase (NOS) knockout strain of Drad (Δnos). We now show that endogenous NO production is also critical for protection of Drad against γ-irradiation (3000 Gy), a result of accelerated growth recovery, not protection against killing. NO-donor treatment rescued radiosensitization in Δnos Drad but did not influence radiosensitivity in wild type Drad. To discover molecular mechanisms by which endogenous NO confers radioresistance, metabolite profiling studies were performed. Untargeted LC-MS-based metabolite profiling in Drad quantified relative abundances of 1,425 molecules and levels of 294 of these were altered by >5-fold (p< 0.01). Unexpectedly, these studies identified a dramatic perturbation in carotenoid biosynthetic intermediates in Δnos Drad, including a reciprocal switch in the pathway end-products from deoxydeinoxanthin to deinoxanthin. NO supplementation rescued these nos deletion-associated changes in carotenoid biosynthesis, and fully-restored radioresistance to wildtype levels. Because carotenoids were shown to be important contributors to radioprotection in Drad, our findings suggest that endogenously-produced NO serves to maintain a spectrum of carotenoids critical for Drad’s ability to withstand radiation insult.

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Section: Discussionsupporting

confidence: 94%

Untargeted metabolite profiling reveals that nitric oxide bioynthesis is an endogenous modulator of carotenoid biosynthesis in Deinococcus radiodurans and is required for extreme ionizing radiation resistance

Hansler

Chen

et al. 2016

Archives of Biochemistry and Biophysics

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“…This situation in A. brasilense is in contrast to D. radiodurans , where bacteriophytochrome is known to control carotenoid synthesis; it accumulated higher amounts of carotenoids under white/red light, whereas the synthesis of carotenoids was severely inhibited in the BphP1 mutant. The bacteriophytochrome found in D. radiodurans thus helps in protecting the bacterium from the adverse effects of intense visible light by controlling the synthesis of the deinoxanthin carotenoid 22 31 . Although AtBphP1 and AtBphP2 of A. tumefaciens are one of the most thoroughly investigated bacteriophytochromes 21 23 24 , their biological role is not known yet.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophytochrome controls carotenoid-independent response to photodynamic stress in a non-photosynthetic rhizobacterium, Azospirillum brasilense Sp7

Kumar

Kateriya

Singh

et al. 2012

Sci Rep

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Ever since the discovery of the role of bacteriophytochrome (BphP) in inducing carotenoid synthesis in Deinococcus radiodurans in response to light the role of BphPs in other non-photosynthetic bacteria is not clear yet. Azospirillum brasilense, a non-photosynthetic rhizobacterium, harbours a pair of BphPs out of which AbBphP1 is a homolog of AtBphP1 of Agrobacterium tumefaciens. By overexpression, purification, biochemical and spectral characterization we have shown that AbBphP1 is a photochromic bacteriophytochrome. Phenotypic study of the ΔAbBphP1 mutant showed that it is required for the survival of A. brasilense on minimal medium under red light. The mutant also showed reduced chemotaxis towards dicarboxylates and increased sensitivity to the photooxidative stress. Unlike D. radiodurans, AbBphP1 was not involved in controlling carotenoid synthesis. Proteome analysis of the ΔAbBphP1 indicated that AbBphP1 is involved in inducing a cellular response that enables A. brasilense in regenerating proteins that might be damaged due to photodynamic stress.

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“…3), a unique ketocarotenoid (Lemee et al 1997;Lysenko et al 2011). There is evidence that the intracellular level of protein oxidation following treatment with H 2 O 2 in a carotenogenesis-deficient mutant was approximately 25 % higher than that in the wild type ).…”

Section: Structures and Roles Of Carotenoids In Some Species Of Deinomentioning

confidence: 99%

Recent progress in understanding the molecular mechanisms of radioresistance in Deinococcus bacteria

2015

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The deleterious effects of ionizing radiation are a major concern of the modern world. In the last decades, outstanding interest has been given to developing new therapeutic tools designed for protection against the toxic effects of ionizing radiation. Deinococcus spp. are among the most radioresistant organisms on Earth, being able to survive extreme doses of radiation, 1000-fold higher than most vertebrates. The molecular mechanisms underlying DNA repair and biomolecular protection, which are responsible for the remarkable radioresistance of Deinococcus bacteria, have been a debatable subject for the last 60 years. This paper is focused on the most recent findings regarding the molecular background of radioresistance and on Deinococcus bacteria response to oxidative stress. Novel proteins and genes involved in the highly regulated DNA repair processes, and enzymatic and non- enzymatic antioxidant systems are presented. In addition, a recently proposed mechanism that may contribute to oxidative damage protection in Deinococcus bacteria is discussed. A better understanding of these molecular mechanisms may draw future perspectives for counteracting radiation-related toxicity.

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Separation and mass spectrometry identification of carotenoid complex from radioresistant bacteria Deinococcus radiodurans

Cited by 8 publications

References 9 publications

Untargeted metabolite profiling reveals that nitric oxide bioynthesis is an endogenous modulator of carotenoid biosynthesis in Deinococcus radiodurans and is required for extreme ionizing radiation resistance

Untargeted metabolite profiling reveals that nitric oxide bioynthesis is an endogenous modulator of carotenoid biosynthesis in Deinococcus radiodurans and is required for extreme ionizing radiation resistance

Bacteriophytochrome controls carotenoid-independent response to photodynamic stress in a non-photosynthetic rhizobacterium, Azospirillum brasilense Sp7

Recent progress in understanding the molecular mechanisms of radioresistance in Deinococcus bacteria

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