Separation and isolation of teratogenic piperidine enantiomers from Nicotiana and Lupinus species.

Abstract

The toxicity of anabasine and ammodendrine enantiomers was evaluated in a mouse bioassay. The LD50 of the L-anabasine fraction in mice was 16.2±1.0 mg/kg, n=26 and that of the D-anabasine fraction was 10.9±0.8 mg/kg, n=26. The LD50 of L-ammodendrine in mice was 115.0±7.0 mg/kg, n=20, and that of D-ammodendrine was 94.1±7.8 mg/kg, n=23. The similarities in toxicity between the enantiomeric pairs of anabasine and ammodendrine indicate that, in the absence of evidence to the contrary, both enantiomers should be taken into account in predicting the toxicity of plant samples to livestock.