Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine

OHTAKE, N; NAKAI, Y; YAMAMOTO, M; SAKAKIBARA, I; TAKEDA, S; AMAGAYA, S; ABURADA, M

doi:10.1016/s1570-0232(04)00547-1

Cited by 50 publications

(35 citation statements)

References 40 publications

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“…5 TJ-9. 17) In Glycyrrhizae Radix, they identified 13 low molecular mass constituents including liquiritin, liquiritin apioside, liquiritigenin, isoliquiritin, isoliquiritin apioside, isoliquiritigenin, glycyrrhizin, glycyrrhizic acid, glycycoumarin, genistein, licopyranocoumarin, licocoumarone and glycyrrhisoflavone. Among these constituents, we tested 7 available components including genistein, licopyranocoumarin, glycyrrhizic acid, liquiritin, liquiritin apioside, isoliquiritin and glycycoumarin at concentrations that do not cause cellular damage.…”

Section: Resultsmentioning

confidence: 99%

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Blockade of the Dioxin Pathway by Herbal Medicine Formula Bupleuri Minor: Identification of Active Entities for Suppression of AhR Activation

Kasai

Hiramatsu

Hayakawa

et al. 2008

Biological & Pharmaceutical Bulletin

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In the daily life, we are exposed to a wide range of environmental pollutants. In particular, dioxins and dioxin-like chemicals are contained in foods and polluted air, and these toxic substances are taken mainly through ingestion and respiration. Cigarette smoke also contains high levels of dioxinlike pollutants that trigger the similar signaling pathway to that of dioxins in vitro and in vivo. 1)Dioxins and dioxin-like substances cause a diverse range of species-and tissue-specific toxicities including carcinogenicity, immunotoxicity, endocrine dysregulation, and reproductive/developmental abnormalities.2,3) Previous reports showed that toxic effects of these pollutants are mediated by the aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor. 4) When cells are exposed to these toxic substances, the toxicants pass through the cell membrane and bind to the cytosolic AhR. Subsequently, the AhR-ligands translocate into the nucleus and heterodimerize with the AhR nuclear translocator. These complexes then bind to the xenobiotic responsive element [XRE, also called dioxin responsive element (DRE)], leading to transcriptional induction of dioxin-responsive genes including cytochrome P450 1A1 (CYP1A1). 5) Previous investigation using AhR-null mutant mice confirmed a critical role of AhR in the toxic effects of dioxins.6) Furthermore, constitutive activation of AhR via genetic manipulation caused malignant tumors and immune abnormality in mice even without exposure to xenobiotic ligands. 7,8) Savouret et al. also indicated that activation of AhR by xenobiotic ligands may be a fundamental trigger for cardiovascular diseases.9) Of note, cigarette smoke, a strong activator of AhR, 1) is one of the most famous risk factors for cancers and cardiovascular diseases. Based on this current concept and knowledge, development of AhR antagonists is important for prevention and treatment of AhR-associated diseases caused by dioxins and other xenobiotic ligands.As described, we recently reported that cigarette smoke contains high levels of agonists for AhR using a cell-based reporter assay. Using reporter transgenic mice, we also provided in vivo evidence that exposure of the mice to cigarette smoke causes significant, sustained activation of AhR. 1) Cigarette smoke is known to cause a variety of pathologies including cancers, obstructive pulmonary diseases, atherosclerosis, cardiovascular/cerebrovascular diseases and developmental/reproductive abnormalities. 10) Many of these pathologies are also caused by dioxins and dioxin-like chemicals. 3,9) The fact that cigarette smoke exhibited high levels of the dioxin-like potential for AhR activation suggested a possibility that the health problems known in smokers are, at least in part, due to the potential of cigarette smoke to activate the AhR-XRE/DRE pathway. Development of AhR antagonists may, therefore, also be useful for prevention of cigarette smoke-related disorders in active and passive smokers. Towards this goal, we aimed in the present study at seeking for ...

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Section: Resultsmentioning

confidence: 99%

“…17) Among these constituents, we tested 5 available components including baicalein, baicalin, wogonin, skullcapflavone II and daidzein at concentrations that do not cause cellular damage. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Blockade of the Dioxin Pathway by Herbal Medicine Formula Bupleuri Minor: Identification of Active Entities for Suppression of AhR Activation

Kasai

Hiramatsu

Hayakawa

et al. 2008

Biological & Pharmaceutical Bulletin

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“…The quality of this drug is controlled by measuring the contents by high-performance liquid chromatography (HPLC). 12,17 Patients Between 2001 and 2005, 47 patients who underwent surgery at Kitano Hospital were divided into two groups: the TJ-41-treated group and the control group. The TJ-41-treated group comprised 20 patients who were administered 7.5 g (2.5 g × 3) TJ-41 every day for 7 days before operation.…”

Section: Hochu-ekki-to (Tj-41)mentioning

confidence: 99%

Preventive effect of a traditional herbal medicine, Hochu-ekki-to, on immunosuppression induced by surgical stress

et al. 2008

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“…Baicalin (1) is an anti-HIV flavonoid obtained from Scutellaria baicalensis which is one of the seven medicinal plants constituting Sho-saiko-to, a traditional Chinese as well as a Japanese medicinal drug (Ohtake et al, 2004). Baicalin inhibits HIV-1 replication in PBMC in a dose dependent manner with IC 50 values of 0.2-0.5 µg/mL and a suitable safety profile; well tolerated up to 10 µg/mL (Kitamura et al, 1998;Li et al, 1993).…”

Section: Flavonoidsmentioning

confidence: 99%

Naturally derived anti‐HIV agents

Asres

Seyoum

Veeresham

et al. 2005

Phytotherapy Research

148

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The urgent need for new anti-HIV/AIDS drugs is a global concern. In addition to obvious economical and commercial hurdles, HIV/AIDS patients are faced with multifarious difficulties associated with the currently approved anti-HIV drugs. Adverse effects, the emergence of drug resistance and the narrow spectrum of activity have limited the therapeutic usefulness of the various reverse transcriptase and protease inhibitors that are currently available on the market. This has driven many scientists to look for new anti-retrovirals with better efficacy, safety and affordability. As has always been the case in the search for cures, natural sources offer great promise. Several natural products, mostly of plant origin have been shown to possess promising activities that could assist in the prevention and/or amelioration of the disease. Many of these anti-HIV agents have other medicinal values as well, which afford them further prospective as novel leads for the development of new drugs that can deal with both the virus and the various disorders that characterize HIV/AIDS. The aim of this review is to report new discoveries and updates pertaining to anti-HIV natural products. In the review anti-HIV agents have been classified according to their chemical classes rather than their target in the HIV replicative cycle, which is the most frequently encountered approach. Perusal of the literature revealed that most of these promising naturally derived anti-HIV compounds are flavonoids, coumarins, terpenoids, alkaloids, polyphenols, polysaccharides or proteins. It is our strong conviction that the results and experiences with many of the anti-HIV natural products will inspire and motivate even more researchers to look for new leads from plants and other natural sources.

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Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine

Cited by 50 publications

References 40 publications

Blockade of the Dioxin Pathway by Herbal Medicine Formula Bupleuri Minor: Identification of Active Entities for Suppression of AhR Activation

Blockade of the Dioxin Pathway by Herbal Medicine Formula Bupleuri Minor: Identification of Active Entities for Suppression of AhR Activation

Preventive effect of a traditional herbal medicine, Hochu-ekki-to, on immunosuppression induced by surgical stress

Naturally derived anti‐HIV agents

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