Separation and investigation of structure–mobility relationship of gonadotropin-releasing hormones by capillary zone electrophoresis in conventional and isoelectric acidic background electrolytes

Šolínová, Veronika; Kašička, Václav; Sázelová, Petra; Barth, Tomislav; Mikšı́k, Ivan

doi:10.1016/j.chroma.2007.01.019

Cited by 37 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present, relevant conclusions on the conformation, size, electrical state, and hydration of polypeptides have been achieved through physicochemical models of their electrophoretic mobility μ p as described, for instance, in and many citations therein. These models have been benefited by modern CZE experimental techniques, which are well discussed in and citations therein.…”

Section: Introductionmentioning

confidence: 99%

Determination of electrokinetic and hydrodynamic parameters of proteins by modeling their electrophoretic mobilities through the electrically charged spherical soft particle

Deiber

Piaggio²,

Peirotti

2013

Electrophoresis

View full text Add to dashboard Cite

This work explores the possibility of using the electrically charged "spherical soft particle" (SSP) to model the electrophoretic mobility of proteins in the low charge regime. The general framework concerning the electrophoretic mobility of the SSP already presented in the literature is analyzed and discussed here in particular for polyampholyte-polypeptide chains. In this regard, this theory is applied to BSA for different protocol pH values. The physicochemical conditions required to model proteins as SSP from their experimentally determined electrophoretic mobilities are established. In particular, the protein charge regulation phenomenon and the SSP particle core are included to study BSA having isoelectric point pI ≈ 5.71, within a wide range of bulk pH values. The results of this case study are compared with previous ones concerning the spherical porous particle and the spherical hard particle with occluded water. A discussion of chain conformations in the SSP polyampholyte layer is presented through estimations of the packing and friction fractal dimensions.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of electrokinetic and hydrodynamic parameters of proteins by modeling their electrophoretic mobilities through the electrically charged spherical soft particle

Deiber

Piaggio²,

Peirotti

2013

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…From the CZE measurements of the dependence of effective mobilities of peptides on the charge/size ratio the probable secondary structure of similar peptides was determined, e.g., the random coil structure was predicted for peptide hormones [246], opioid peptides [25], and GnRHs [30]. The electrophoretic mobilities of a series of dipeptides determined by CZE allowed estimation of their transdermal iontophoretic mobility and to evaluate their suitability for iontophoretic delivery [247].…”

Section: Physicochemical Characterizationmentioning

confidence: 99%

“…They found them promising BGEs for the CZE of proteins and peptides with respect to their low conductivity and good buffering capacity, permitting application of high intensity of electric field and thus achieving short separation times. These properties are provided also by single component isoelectric buffers, such as 100-200 mM iminodiacetic acid, which was used for CZE separation of GnRHs and their analogs and fragments [30].…”

Section: Zementioning

confidence: 99%

“…Linear and nonlinear quantitative structure-property relationship models for prediction of peptide mobilities in CZE were suggested using the linear heuristic method and a nonlinear radial basis function indicate dependencies measured in five acidic BGEs, pH 2.18-2.50. Adapted with permission from [30].…”

Section: Introductionmentioning

confidence: 98%

“…(1) introduced by Cross et al [28,29] log m ep q ¼ Àk log M r (2) have been used to investigate the structure-mobility relationship of gonadotropin-releasing hormones (GnRHs) and their analogs and fragments [30]. None of the models was found to be quite definitively applicable for the whole set of ten GnRHs differing in size (tetrapeptide to decapeptide) and charge (10.9 to 13.0 elementary charges) which were analyzed in five conventional and isoelectric acidic BGEs, pH 2.18-2.50.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

Self Cite

118

View full text Add to dashboard Cite

The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

show abstract