2008
DOI: 10.1007/s12010-008-8425-7
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Separation and Immobilization of Lipase from Penicillium simplicissimum by Selective Adsorption on Hydrophobic Supports

Abstract: Lipases are an enzyme class of a great importance as biocatalysts applied to organic chemistry. However, it is still necessary to search for new enzymes with special characteristics such as good stability towards high temperatures, organic solvents, and high stereoselectivity presence. The present work's aim was to immobilize the lipases pool produced by Penicillium simplissicimum, a filamentous fungi strain isolated from Brazilian babassu cake residue. P. simplissicimum lipases were separated into three diffe… Show more

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Cited by 26 publications
(17 citation statements)
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References 27 publications
(36 reference statements)
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“…Cunha et al [83] studied the purification/immobilization of a “pool” of lipases from P. simplicissimum produced by SSF using babassu cake as a culture medium. The process undertaken by means of sequential immobilization in hydrophobic supports (butyl, phenyl, and octyl agarose) resulted in three fractions with distinct thermal stability, specificity, and enantioselectivity properties.…”
Section: Enzyme Production and Characteristicsmentioning
confidence: 99%
“…Cunha et al [83] studied the purification/immobilization of a “pool” of lipases from P. simplicissimum produced by SSF using babassu cake as a culture medium. The process undertaken by means of sequential immobilization in hydrophobic supports (butyl, phenyl, and octyl agarose) resulted in three fractions with distinct thermal stability, specificity, and enantioselectivity properties.…”
Section: Enzyme Production and Characteristicsmentioning
confidence: 99%
“…Enzyme immobilization ensures recycling of the biocatalyst, allows easy product separation, and may improve performance of the enzyme [4]. The kinetic behavior of free enzymes is different from the one shown by immobilized forms due to conformational changes, steric hindrance, substrate and products partition, and microenvironmental and diffusion effects.…”
Section: Introductionmentioning
confidence: 99%
“…The immobilization was conducted for 25 h at 17 °C under mild stirring (36 rpm). During enzyme immobilization, aliquots of 150 µL were withdrawn (0, 0.5, 1, 3, 6, 25 h) for determination of the hydrolytic activity of the enzyme still present in the supernatant, using p ‐NPL as substrate . Finally, the biocatalysts (immobilized enzymes) obtained at the end of the immobilization process were washed with distilled water, filtrated, dried in desiccators, and stored in the refrigerator (17 °C).…”
Section: Methodsmentioning
confidence: 99%