Separation and identification of peptide mixtures in a synthesis crude of carbetocin by liquid chromatography/electrospray ionization mass spectrometry

Sanz‐Nebot, Victoria; Toro, I.; Garces, Alejandra; Barbosa, Johildo Salomão Figueirêdo

doi:10.1002/(sici)1097-0231(19991215)13:23<2341::aid-rcm795>3.0.co;2-9

Cited by 13 publications

(4 citation statements)

References 28 publications

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“…2, indicating that only two experimental measurements per compound are enough to predict accurately the chromatographic behaviour and to optimize the mobile phase composition, in accordance with the results obtained in previous work 11–15,. 34–37…”

Section: Resultssupporting

confidence: 88%

Investigation of synthetic peptide hormones by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry: analysis of a synthesis crude of peptide triptorelin

Sanz‐Nebot

Toro

Castillo

et al. 2001

Rapid Comm Mass Spectrometry

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Triptorelin, a synthetic peptide hormone used in the treatment of prostate cancer by means of reduction in the action of male hormone testosterone, is studied here. The synthetic procedure commonly results in unwanted side products that require extensive purification and characterization of the synthesis mixture. The chromatographic separation of triptorelin from the crude mixture was developed by applying the linear solvation energy relationship (LSER) methodology previously developed, to optimize the composition of the mobile phase in order to avoid lengthy empirical optimization procedures. Electrospray ionization mass spectrometry coupled to liquid chromatography (LC/ES-MS) was used to obtain reliable information on the inevitable side products. The knowledge of the identity of these impurities allows fast optimization of the synthetic procedure and also the therapeutic use of triptorelin peptide hormone.

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Section: Resultssupporting

confidence: 88%

Investigation of synthetic peptide hormones by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry: analysis of a synthesis crude of peptide triptorelin

Sanz‐Nebot

Toro

Castillo

et al. 2001

Rapid Comm Mass Spectrometry

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“…Carbetocin (1‐butanoic acid‐2‐( O ‐methyl‐L‐tyrosine)‐1‐carbaoxytocin) is a synthetic analog of oxytocin used to prevent and treat postpartum hemorrhage (Atke & Vilhardt, ; Hunter, Schulz, & Wassenaar, ). It is a cost‐effective alternative for treating weak uterine contraction because it is long‐lasting and requires a low dose (Caceda, Ramos, & Saborido, ; Hunter et al, ; Sanz‐Nebot, ). It has been used in human, pigs, horses, sheep, goats, and cattle due to having a higher stability than oxytocin (Boonraungroda et al, ; Cort, Einarsson, Schams, & Vilhardt, ; Gimpl, Postina, Fahrenholz, & Reinheimer, ; Henríquez‐Trujillo, Lucio‐Romero, & Bermúdez‐Gallegos, ; Voon, Shafie, Bujang, & Suharjono, ).…”

Section: Pharmacokinetic Parameters (Mean ± Sd) For Carbetocin In Cowmentioning

confidence: 99%

Pharmacokinetics and bioavailability of carbetocin after intravenous and intramuscular administration in cows and gilts

Sun

Xiao

Wang

et al. 2019

Vet Pharm & Therapeutics

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The pharmacokinetics of carbetocin, which is used to control postpartum hemorrhage after giving birth, was studied in cows and gilts after a single intravenous (IV) or intramuscular (IM) injection. Blood samples from animals were assessed by oxytocin radioimmunoassay, and then the pharmacokinetic parameters were calculated using a noncompartmental model. For gilts, there was no significant difference between half‐life (T1/2λZ), mean residue time (MRT), and maximum concentration (Cmax) between IM and IV administration. Conversely, the time to reach the Cmax (Tmax) and MRT were higher following administration of 350 μg/animal in cows via the IM administration compared with IV. The longest T1/2λZ was 0.85 hr, indicating carbetocin was absorbed and eliminated rapidly in both animal species after administration. The Tmax was similar between cows and gilts following IM administration. Moreover, the Cmax after IM injection was about half that of IV administration in both animals. The bioavailability was more than 80% in cows, suggesting administration via the IM route is efficient. This is in agreement with the longer T1/2λZ in cows after IM administration. However, the IV route is recommended for gilts due to a lower bioavailability (35%) and shorter T1/2λZ after IM administration compared with IV.

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“…Moreover, collision‐induced dissociation (CID) fragmentation increases structural information. Several reports have been published on the LC/ESMS characterization of different biomolecules,17–20 but there are limited reports on the successful on‐line LC/MS analysis of complex diuretic mixtures 21, 22. In this work, we attempted to establish a versatile method for the characterization and determination of diuretics in human urine samples by LC coupled to pneumatically assisted ESMS.…”

Section: Introductionmentioning

confidence: 99%

Determination and characterization of diuretics in human urine by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry

et al. 2001

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The aim of this work was to develop a method for the characterization and determination of diuretics in human urine samples by liquid chromatography (LC) coupled to pneumatically assisted electrospray ionization (ES) mass spectrometry (MS). The diuretics studied were substances forbidden by the IOC such as trichlormethiazide, furosemide, canrenoic acid, benzthiazide, bendroflumethiazide, bumetanide, etacrynic acid and spironolactone. For this purpose, the operational parameters of electrospray, such as counter electrode voltage, capillary voltage, sample cone voltage and source temperature, were optimized in order to obtain the best signal stability and the highest sensitivity for the greatest number of diuretic agents. The optimized separation method was successfully coupled with the MS system to analyze the above-mentioned diuretics extracted from spiked urine samples by a liquid extraction and clean-up procedure at basic pH, using ethyl acetate as solvent and the salting-out effect (NaCl). The mass spectra obtained provide adequate information for identification purposes. Positive urine samples obtained from athletes were also analyzed. The presence of these substances in human urine was confirmed by this method, making LC/ES-MS an analytical tool to be considered in the area of antidoping control.

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Separation and identification of peptide mixtures in a synthesis crude of carbetocin by liquid chromatography/electrospray ionization mass spectrometry

Cited by 13 publications

References 28 publications

Investigation of synthetic peptide hormones by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry: analysis of a synthesis crude of peptide triptorelin

Investigation of synthetic peptide hormones by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry: analysis of a synthesis crude of peptide triptorelin

Pharmacokinetics and bioavailability of carbetocin after intravenous and intramuscular administration in cows and gilts

Determination and characterization of diuretics in human urine by liquid chromatography coupled to pneumatically assisted electrospray ionization mass spectrometry

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